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Troubleshooting Support for HPLC

Having issues with your HPLC system? Our troubleshooting guide offers solutions for common problems, ensuring optimal performance in your analytical processes.

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How Do Bioinert HPLC & UHPLC Columns Work?

Bioinert HPLC and UHPLC columns are designed to prevent interactions between metal-sensitive compounds and the column hardware, ensuring highly accurate and reproducible results. Standard stainless steel columns can lead to adsorption effects, causing peak broadening, signal loss, or reduced recovery—especially for biomolecules like (oligo)nucleotides, phosphopeptides, proteins, and phospholipids.

YMC offers two bioinert column technologies to address these challenges:

  • YMC Accura Columns: Featuring a specialized bioinert surface coating, these columns prevent metal interactions and offer high sensitivity without requiring preconditioning.

  • PEEK-lined Stainless Steel Columns: These fully bioinert columns use a PEEK-lined interior and PEEK frits, providing a metal-free flow path while maintaining high-pressure stability.

 

Why Use Bioinert HPLC & UHPLC Columns?

Bioinert columns are essential for applications requiring ultra-high purity analysis and reliable peak shapes, particularly in LC/MS workflows. They prevent carry-over effects, ensure maximum sample recovery, and allow for highly sensitive biomolecule separations. By eliminating metal-induced interactions, YMC bioinert HPLC columns enhance analytical precision for pharmaceutical, biotechnology, and life science applications.

 

For expert advice on selecting the right bioinert HPLC & UHPLC columns, YMC Europe is here to help. Contact us for tailored solutions and ensure optimal performance in your bioanalytical applications.

Contact us for consultation
  • YMC Europe GmbH
  • Phone +49 2064 427-0
  • Schöttmannshof 19
  • Fax +49 2064 427-222
  • D-46539 Dinslaken