(U)HPLC Method Development and Validation Kits
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Efficient HPLC method development and validation require reliable tools to ensure reproducible results. To support this process, YMC now offers attractively priced (U)HPLC Method Development and Validation Kits.
Validation kit*
3 columns from 3 different batches (to allow assessment of reproducibility across a wide range of gel batches)
Part number
Just expand the part number for the desired column by adding V2. E.g. Method validation kit for YMC-Triart C18, 1.9 µm, 50 x 2.1 mm (TA12SP9-05Q1PT) from 3 different lots: TA12SP9-05Q1PTV2
Pricing
10% less than 3 single columns.
*available for selected phases only (YMC-Triart, BioPro, CHIRAL ART and selected others)
(U)HPLC Method Development Kits
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In order to offer a convenient solution for method developers, YMC is now offering different price attractive Method Development Kits for both achiral and chiral separations.
Available dimensions pre-composed kits
Particle size: 1.9 or 3 µm
Length: 50 or 150 mm (for CHIRAL ART)
ID: 2.0/2.1 or 4.6 mm (for CHIRAL ART)
Pricing
The kits are available at a special price compared to the list price for 3 single columns.
Options
- Choose from a selection of 3 column chemistries
- Get YMC-Triart, YMC-Pack Pro or CHIRAL ART columns
- Have a choice of different column dimensions
- Pre-composed or individual kits
- Benefit from very attractive pricing
Technical Library
| Title | Document Type | Language | Download |
 Analytical Stationary Phases for Achiral and Chiral SFC/LC from YMC |
Overview | EN | |
| Analytical Stationary Phases for BioLC from YMC |
Overview | EN | |
| Analytical Stationary Phases for RP HPLC and UHPLC from YMC |
Overview | EN | |
 Allowable Adjustments to HPLC Methods in the European Pharmacopoeia (Ph.Eur.) |
Technical Note | EN | |
| Erlaubte Änderungen in HPLC Methoden aus der Europäischen Pharmakopöe (Ph.Eur.) 11. Edition |
Technical Note | DE |
Ordering Information
Please login to see the prices.
- 2-3 days
- 1-2 weeks
- 3-4 weeks
| Product | Description | Price | Stock |
| ASHSRSS03-0502WT | YMC-Pack Pro C18, C18 RS, Hydrosphere C18, Method Development Kit (2.0 mm ID), S-3 µm, 50 x 2.0 mm | - 2451 CHF 1999 € | |
| KSAKSBKSCS03-0546WT | CHIRAL ART Amylose-SA, Cellulose-SB, Cellulose-SC, Method Development Kit (4.6 mm ID), S-3 µm, 50 x 4.6 mm | - 4119 CHF 3360 € | |
| KSAKSBKSCS03-1546WT | CHIRAL ART Amylose-SA, Cellulose-SB, Cellulose-SC, Method Development Kit (4.6 mm ID), S-3 µm, 150 x 4.6 mm | - 5966 CHF 4866 € | |
| TATARTPHS03-05Q1PTH | YMC-Triart C18, C18 ExRS, Phenyl, HPLC Method Development Kit (2.1 mm ID), S-3 µm, 50 x 2.1 mm | - 2161 CHF 1763 € | |
| TATARTPHSP9-05Q1PT | YMC-Triart C18, C18 ExRS, Phenyl, UHPLC Method Development Kit (2.1 mm ID), S-1.9 µm, 50 x 2.1 mm | - 3194 CHF 2605 € | |
| TATOTPHS03-05Q1PTH | YMC-Triart C18, C8, Phenyl, HPLC Method Development Kit (2.1 mm ID), 12 nm, S-3 µm, 50 x 2.1 mm | - 2161 CHF 1763 € | |
| TATOTPHSP9-05Q1PT | YMC-Triart C18, C8, Phenyl, UHPLC Method Development Kit (2.1 mm ID), 12 nm, S-1.9 µm, 50 x 2.1 mm | - 3194 CHF 2605 € | |
| TATPFTDHS03-05Q1PTH | YMC-Triart C18, PFP, Diol-HILIC, HPLC Method Development Kit (2.1 mm ID), 12 nm, S-3 µm, 50 x 2.1 mm | - 2161 CHF 1763 € | |
| TATPFTDHSP9-05Q1PT | YMC-Triart C18, PFP, Diol-HILIC, UHPLC Method Development Kit (2.1 mm ID), 12 nm, S-1.9 µm, 50 x 2.1 mm | - 3194 CHF 2605 € |
HPLC Method Development and Validation – Best Practices
Reliable and reproducible HPLC method development and validation are essential for generating robust analytical data – especially in pharmaceutical, biotechnological and chemical applications. Whether for routine quality control or method transfer, a validated HPLC method ensures consistent performance across instruments, analysts, and production batches.
What defines a good HPLC method?
HPLC method development involves selecting appropriate stationary phases, optimizing mobile phase composition, flow rate, temperature, and detection settings to achieve ideal separation, peak shape, and run time. Robustness testing under slightly varying conditions is also a key part of this phase. Once a method has been optimized, HPLC validation confirms that it performs reliably under defined conditions. Typically aligned with ICH Q2(R1) guidelines, validation includes parameters such as accuracy, precision, linearity, limit of detection (LOD), limit of quantification (LOQ), specificity, and robustness.
Why batch-to-batch reproducibility matters
Even small variations in the silica base or bonding chemistry between column lots can affect retention times or resolution. Therefore, assessing batch-to-batch reproducibility is critical for regulated environments. YMC’s Validation Kits address this requirement by including three columns from three different production lots – helping ensure long-term consistency and method reliability.
FAQ – HPLC Method Development and Validation
What is the difference between HPLC method development and validation?
Method development focuses on optimizing separation conditions. Validation confirms the method performs reliably and reproducibly.
Why are multiple column batches used during HPLC validation?
To assess robustness and confirm that method performance is consistent across different stationary phase batches.
Are YMC kits suitable for validated HPLC methods in regulated settings?
Yes. They are designed to support reproducibility and batch-to-batch consistency in line with regulatory expectations.
Can I customize the kits for specific method development needs?
Yes. YMC offers both predefined and configurable kits for achiral and chiral separations.
Looking for additional support?
In addition to our ready-to-use (U)HPLC Method Development and Validation Kits, YMC also offers expert services for method development, phase screening, and method optimization.