HT Glass Columns

    YMC HT Glass Columns are specially designed to accommodate the stringent requirements of high-temperature liquid chromatography applications. Capable of performing efficiently at temperatures up to 70 °C, these columns are perfectly suited for a broad spectrum of purification tasks, particularly in biochemical liquid chromatography (BioLC), where high temperatures are pivotal. The glass tempering jacket integrated into each column ensures precise temperature management, leading to consistent and dependable chromatographic outcomes.
    Engineered for adaptability, HT Glass Columns are compatible with all standard separation modes including normal phase, reversed phase, and ion-exchange chromatography, and can be used with a wide array of resins. This makes them an essential tool for scientists and technicians conducting high-temperature separations. Featuring straightforward operation and robust construction for enduring use at elevated temperatures, the HT series stands as a premier solution for sophisticated chromatographic analyses.

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    Features

    Qualitative High Temperature Line

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    High Temperature Stability

    YMC HT is designed for high temperature applications.

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    For All Type of Resins

    YMC HT is dedicated for all common separation modes in BioLC that require elevated temperatures.

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    Optimal Flow Distribution

    The frit technology used with YMC HT allows an optimal flow distribution.

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    Glass Tempering Jacket

    The tempering jacket of YMC HT is made of glass.

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    Benefits in Detail

    Specifications

    HT Glass Column Portfolio

    Inner Diameters [mm]
    10, 15, 25, 50
    Maximum Bed Lengths [mm]
    120, 200, 300, 450, 750, 1000
    Pressure Range [bar]
    30–10 (depending on inner diameter)
    Temperature Range [°C]
    4–70
    Full Specifications

     

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    Your benefits

    High Temperature Stability

    YMC HT is designed for high temperature applications. The column is specified up to 70 °C which makes it the first choice for purifications under elevated temperatures. All components are extremely robust and stable for long-term usage with high temperatures.

    For All Type of Resins

    YMC HT is dedicated for all common separation modes in BioLC that require elevated temperatures - especially for the purification of oligonucleotides using AEX, RP or HIC. YMC HT is compatible with all these types of separations and the resins used for these processes.

    Optimal Flow Distribution

    The frit technology used with YMC HT allows an optimal flow distribution. This leads to great packing results and chromatographic performance. The frits can easily be replaced for a long-term usage of YMC HT.

    Glass Tempering Jacket

    The tempering jacket of YMC HT is made of glass. This enables reliable tempering of the column during the process. Additionally, full organic solvent compatibility provides an efficient cleaning of the tempering jacket.

    Specifications of YMC HT Glass Columns

    10 mm ID
      Column dimensions
    Maximum bed length
    [mm]
    		Pressure limit
    [bar]
    		Bed length range
    [mm]
    		Volume range
    [mL]
    120
    30
    		15 - 1201.5 - 9
    2003095 - 2009 - 15
    30030195 - 30017 - 23
    45030345 - 45030 - 35
    75030645 - 75055 - 58
    100030905 - 100077 - 78
    15 mm ID
      Column dimensions
    Maximum bed length
    [mm]
    		Pressure limit
    [bar]
    		Bed length range
    [mm]
    		Volume range
    [mL]
    1202510 - 1202 - 19
    2002590 - 20018 - 32
    30025190 - 30037 - 49
    45025335 - 45065 - 73
    75025635 - 750121 - 122
    100025885 - 1000163 - 170
    25 mm ID
      Column dimensions
    Maximum bed length
    [mm]
    		Pressure limit
    [bar]
    		Bed length range
    [mm]
    		Volume range
    [mL]
    1201510 - 1206 - 60
    2001590 - 20055 - 100
    30015195 - 300113 - 156
    45015345 - 450200 - 230
    75015645 - 750375 - 390
    100015895 - 1000515 - 520
    50 mm ID
      Column dimensions
    Maximum bed length
    [mm]
    		Pressure limit
    [bar]
    		Bed length range
    [mm]
    		Volume range
    [mL]
    1201020 - 12045 - 235
    20010100 - 200225 - 390
    30010205 - 300453 - 593
    45010355 - 450785 - 885
    75010655 - 7501450-1480
    100010905 - 10001970-2000

    Characteristics

    Parameter Standard columns
    Temperature range 4 - 70°C
    Wetted parts PEEK pistons, glass frits, FFKM O-ring, borosilicate glass 3.3
    Connections The columns are supplied with different adapters to allow direct connection to any LC-system.
    Accessories supplied for columns with
    ID 10-15 mm
    		 1 x frit removal tool
    2 x plugs, PTFE (1/4"-28G)
    1 x 1 m FEP tubing (0.8 x 1.6 mm)
    4 x 1/4"-28G nut and ferrule for 1/16" tubing (collapsible)
    2 x M6 nut and ferrule for 1/16" tubing
    2 x 10-32 nut and ferrule for 1/16" tubing
    2 x anti-kink tube protector
    Accessories supplied for columns with
    ID 25-50 mm
    		 1 x frit removal tool
    2 x plugs, PTFE (1/4"-28G)
    1 x 1 m FEP tubing (1.6 or 2.4 x 3.2 mm)
    4 x 1/4"-28G nut and ferrule for 1/8" tubing (collapsible) 
    2 x M6 nut and ferrule for 1/8" tubing

    Accessories for YMC HT columns

    Packing Adapters

    Packing adapters are used if the packing method requires additional column volume during the packing process. More volume than given by the column alone may be required when the column is packed with the slurry method. A suspension of material and packing solvent in a concentration of approximately 30 - 50% is used, depending on the packing material. The additional packing solvent above the finished column bed will be removed later but during the packing process the packing adapter gives the additional volume which is required for this method.

    The ECO and YMC HT series packing adapters consist of a column coupler and an empty glass body. The packing adapter is installed as follows:

    • Remove the upper end piece from the column body.
    • Put the counter screw of the coupling unit on the column body. Take care that just the blue column coupler snap ring is included.
    • Install the end piece on top of the glass body of the packing adapter.

    Video on the Assembly of packing adapters

    Watch Video

    Column Stands

    For optimum positioning next to your system, YMC offers column stands for YMC HT glass columns. Due to the reliable and handy design, setting up the column was never faster and easier. No tools are required for secured fixation!

     

    Your advantages are:

    • Stands for 25 and 50 mm ID
    • High stability: Screwed in feet for secure grip
    • Perfect alignment: The feet are equipped with leveling feet

    Handling Loop

    This tool helps you to open and to close YMC glass columns. The Handling Loop is universally applicable to all available inner diameters.

    • Simplified opening of glass columns
    • Universally applicable
    • Easy grip especially with larger inner diameters

    Adapter for 10-32 fitting

    YMC have specifically designed an adapter to allow easy connection of YMC glass columns to ÄKTA systems. This adapter will allow direct connection of the 10-32 fittings of the ÄKTA systems to the inlets and outlets of YMC glass columns.

    This adapter is compatible to all existing YMC glass columns with internal diameters between 10 to 50 mm and can be ordered as a pack of 2 pieces.

    Title Document Type Language Download
    BrochureYMC Glass Columns For Laboratory Scale Applications Brochure EN
    Download
    BrochureYMC HT - High Temperature Glass Columns Brochure EN
    Download
    Expert TipPacken einer 10 mm ID-Säule mit BioPro IEX SmartSep S30 Expert Tip DE
    Download
    Expert TipPacking a 10 mm ID column with BioPro IEX SmartSep S30 Expert Tip EN
    Download
    FlyerECO-Säulenstandfüße für eine optimale Säulenpositionierung Flyer DE
    Download
    FlyerGlass Column Packing Service by YMC Flyer EN
    Download
    FlyerGlassäulen-Packservice YMC Flyer DE
    Download
    FlyerOptimum Column Positioning with ECO Column Stands! Flyer EN
    Download
    ManualBedienungsanleitung ECO Glassäulen Manual DE
    Download
    ManualBedienungsanleitung Handling-Loop für YMC-Glassäulen Manual DE
    Download
    ManualBedienungsanleitung YMC HT Glassäulen Manual DE
    Download
    ManualGlass Columns’ Logbook – Cleaning Manual EN
    Download
    ManualGlass Columns’ Logbook – Maintenance Manual EN
    Download
    ManualGlass Columns’ Logbook – Operation Manual EN
    Download
    ManualInstructions for Frit Replacement 70 & 80 mm ID ECO columns Manual EN
    Download
    ManualKurzanleitung YMC Glassäulen für den Labormaßstab - ECO Manual DE
    Download
    ManualKurzanleitung YMC Glassäulen für den Labormaßstab - YMC HT Manual DE
    Download
    ManualLogbuch Glassäulen – Betrieb Manual DE
    Download
    ManualLogbuch Glassäulen – Reinigung Manual DE
    Download
    ManualLogbuch Glassäulen – Wartung Manual DE
    Download
    ManualManual ECO Glass Columns Manual EN
    Download
    ManualManual Handling-Loop for YMC Glass Columns Manual EN
    Download
    ManualManual YMC HT Glass Columns Manual EN
    Download
    ManualQuick Guide YMC Glass Columns for Laboratory Scale - ECO Manual EN
    Download
    ManualQuick Guide YMC Glass Columns for Laboratory Scale - YMC HT Manual EN
    Download
    ManualSchritt-für-Schritt-Anleitung Packen von Laborglassäulen Manual DE
    Download
    ManualStep-by-Step Guide to Packing Lab Scale Glass Columns Manual EN
    Download
    OverviewECO Glass Columns | Overview Overview EN
    Download
    OverviewECO Glassäulen | Übersicht Overview DE
    Download
    OverviewYMC Glass Columns for Lab and Pilot Scale Overview EN
    Download
    PosterAccessories for YMC Glass Columns Poster EN
    Download
    PosterEinfaches Glassäulen-Packen mit Softgelen Poster DE
    Download
    Product InformationEasy Connection of YMC Glass Columns to Äkta systems Product Information EN
    Download
    Product InformationEmpty glass columns for temperature sensitive purifications Product Information EN
    Download
    Product InformationGlassäulen für temperatursensitive Applikationen Product Information DE
    Download
    Product InformationHandling-Loop for YMC Glass Columns Product Information EN
    Download
    Product InformationHandling-Loop für YMC-Glassäulen Product Information DE
    Download
    Product InformationPackadapter – Praktische Helfer beim Packen von Glassäulen Product Information DE
    Download
    Product InformationPacking adapter – Handy accessory for packing glass columns Product Information EN
    Download
    Product InformationPerfekte Flussverteilung mit YMC Glassäulen Product Information EN
    Download
    Product InformationPerfekte Flussverteilung mit YMC Glassäulen Product Information DE
    Download
    QuestionnaireQuestionnaire for YMC Glass Columns (Lab and Pilot Scale) Questionnaire EN
    Download
    WhitepaperCleaning and Cleaning Validation of Glass Columns Laboratory and Pilot Scale Whitepaper EN
    Download

    Request Now

    Contact us via phone or use the below questionnaire to get the right column for your projects.

    Anna Rousek

    Product Manager Glass Columns

     

    ph. +49 2064 427-272

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    • YMC Europe GmbH
    • Phone +49 2064 427-0
    • Schöttmannshof 19
    • Fax +49 2064 427-222
    • D-46539 Dinslaken
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