Available phases are:
BioPro HIC columns are characterised by:
- High resolutions
- Short run times
- High throughput
- Excellent lot-to-lot reproducibility
- Long term stability
- No carryover effects
Applications
| Application | Column | Compound name | |
| Antibody drugs by HIC using Isopropanol | BioPro HIC BF | Adalimumab (Humira®) Brentuximab Pembrolizumab (Keytruda®) Reslizumab (Cinqaero®) | |
| Antibody drugs by HIC using ammonium sulphate | BioPro HIC BF | Bevacizumab (Avastin®) Rituximab (MabThera®) NIST mAb Trastuzumab (Herceptin®) Adalimumab (Humira®) | |
| Antibody drugs by HIC using sodium chloride | BioPro HIC BF | Bevacizumab (Avastin®) Rituximab (MabThera®) NIST mAb Trastuzumab (Herceptin®) Adalimumab (Humira®) | |
| Drug-to-Antibody Ratio (DAR) analysis of ADC | BioPro HIC BF | Cysteine-conjugated ADC mimic | |
| High throughput DAR determination of brentuximab vedotin (Adcetris®) by reducing the analysis time | BioPro HIC HT | Brentuximab vedotin (Adcetris®) | |
| Online native HIC-MS analysis of a monoclonal antibody mixture | BioPro HIC BF | 7 different Monoclonal Antibodies | |
| Online native HIC-MS analysis of a monoclonal antibody molecular variants | BioPro HIC BF | 2 different Monoclonal Antibodies | |
| Online native HIC-MS analysis of cys-linked ADCs | BioPro HIC BF | Cysteine conjugated ADC mimic deglycosylated | |
| Protein Analysis by HIC | BioPro HIC BF | Myoglobin Ribonuclease A Lysozyme α-Chymotrypsinogen A |
Specifications
|
BioPro HIC HT
|
BioPro HIC BF
|
|
| Base Material | hydrophilic polymer (polymethacrylate) | |
| Particle Size [µm] | 2.3 | 4 |
| Pore Size | non-porous | |
| pH Range | 2.0 - 12.0 | |
| Pressure Limit | 40 MPa / 5,800 psi | 20 MPa / 2,900 psi |
| Temperature Range | 10 - 60°C | |
Phase Details
BioPro HIC HT
| Base Material | hydrophilic polymer (polymethacrylate) |
| Particle Size | 2.3 µm |
| Pore Size | non-porous |
| Modification | butyl |
| pH Range | 2.0 - 12.0 |
| Pressure Limit | 40 MPa / 5,800 psi |
| Temperature Range | 10 - 60°C |
BioPro HIC BF
| Base Material | hydrophilic polymer (polymethacrylate) |
| Particle Size | 4 µm |
| Pore Size | non-porous |
| Modification | butyl |
| pH Range | 2.0 - 12.0 |
| Pressure Limit | 20 MPa / 2,900 psi |
| Temperature Range | 10 - 60°C |
Technical Library
| Title | Document Type | Language | Download |
 Analysis of oxidised monoclonal antibodies using BioPro HIC BF |
Application Note | EN | |
| HIC Analysis of Different Monoclonal Antibodies using Isopropanol as Modifier |
Application Note | EN | |
| HPLC Analysis of Drug-to-Antibody Ratio with BioPro HIC BF by YMC |
Application Note | EN | |
| High throughput DAR determination of brentuximab vedotin using YMC's BioPro HIC HT |
Application Note | EN | |
| Online native HIC-MS analysis of MAbs and their molecular variants using BioPro HIC BF |
Application Note | EN | |
| Online native HIC-MS analysis of cys-linked ADCs using BioPro HIC BF |
Application Note | EN | |
| Separation of plasmid isoforms using BioPro HIC BF |
Application Note | EN | |
| The right HIC column for each separation- BioPro HIC HT and BioPro HIC BF |
Application Note | EN | |
| YMC Application Collection - Antibodies & ADCs |
Application Note | EN | |
 BioPro HIC HT and BioPro HIC BF Care and Use |
Care and Use Instructions | EN | |
 YMC Biochromatography Columns |
Catalogue | EN | |
| YMC Biochromatography Columns (HQ) |
Catalogue | EN | |
| YMC Biochromatography Columns - HIC Columns Chapter |
Catalogue | EN | |
 Efficient HIC plasmid isoform analysis |
Expert Tip | EN | |
 Analytical Stationary Phases for BioLC from YMC |
Overview | EN | |
| YMC Alternatives to Existing BioLC Columns |
Overview | EN | |
 The Principles of BioLC & YMC BioLC Phase Overview |
Poster | EN | |
 About the resolving power of non-porous HIC columns for intact protein analysis |
Technical Note | EN | |
| Characterisation of mAbs and related products via HIC-MS |
Technical Note | EN | |
| Charakterisierung von monoklonalen Antikörpern und verwandten Produkten via HIC-MS |
Technical Note | DE | |
| Strategies to Improve Your HIC Analysis |
Technical Note | EN | |
| Welcher LC-Modus ist besser für verschiedene ADC-Typen geeignet? RP oder HIC? |
Technical Note | DE | |
| Which LC-mode is more suitable for different ADC-types? RP or HIC? |
Technical Note | EN | |
 Grundlagen der Chromatographie für die Analyse und Aufreinigung von Biologika |
Whitepaper | DE |
Ordering Information
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- 2-3 days
- 1-2 weeks
- 3-4 weeks
| Product | Description | Price | Stock |
| BHB00S04-1010WT | BioPro HIC BF, non-porous, semi-preparative HPLC column (10 mm ID), S-4 µm, 100 x 10 mm | - 14831 CHF 12097 € | |
| BHB00S04-1046WT | BioPro HIC BF, non-porous, HPLC column (4.6 mm ID), S-4 µm, 100 x 4.6 mm | - 2137 CHF 1743 € | |
| BHH00SQ3-1046PTH | BioPro HIC HT, non-porous, HPLC column (4.6 mm ID), S-2.3 µm, 100 x 4.6 mm | - 2367 CHF 1931 € | |
| BHH00SQ3-H346PTH | BioPro HIC HT, non-porous, HPLC column (4.6 mm ID), S-2.3 µm, 33 x 4.6 mm | - 1828 CHF 1491 € | |
| XRPRCP02 | Precolumn filter 2 µm for 1/16" OD + holder, PEEK (350 bar) | - 353 CHF 288 € | |
| XRPRCP25 | Replacement precolumn filter 2 µm for 1/16" OD, PEEK (350 bar) | - 257 CHF 210 € | |
| XRPRCS03 | Precolumn filter 0.5 µm for 1/16" OD + holder, stainless steel (600 bar) | - 353 CHF 288 € | |
| XRPRCS35 | Replacement precolumn filter 0.5 µm for 1/16" OD, stainless steel (600 bar) | - 257 CHF 210 € |
