For High Resolution Applications

BioPro IEX: Ion Exchange Columns

Ion exchange chromatography (IEX) is widely used for analysis and purification of biomolecules. BioPro ion exchange columns are specifically designed for separation of proteins, peptides and nucleic acids.
BioPro IEX columns are based on porous and non-porous hydrophilic polymer beads with low nonspecific adsorption.
They also show higher binding capacity and higher recovery of biomolecules compared to conventional ion exchange columns. The completely spherical, monodisperse beads, together with optimal packing technology, provide high theoretical plate numbers and symmetrical peak shapes!

Available phases are:

BioPro Ion Exchange Columns are characterised by:

Non-porous or porous
particles
High loadability and
recovery
Very high resolution
High dynamic binding capacity
Long term stability
Excellent lot-to-lot
reproducibility

Applications

Application	Column	Compound name
DNA fragments	BioPro IEX QF	DNA fragments	View Download
Lambda DNA Hind Ⅲ/EcoRⅠ restriction fragments	BioPro IEX QF	Lambda DNA Hind III/EcoR I digest (130-23, 130 bp)	View Download
Monoclonal antibody (MAb)	BioPro IEX SF	Humanized monoclonal IgG1	View Download
Peptide mapping	BioPro IEX QA	Peptide mapping	View Download
Plasmid pBR322 and pBR322 Hae III restriction fragments	BioPro IEX QF	Plasmid pBR322 Hae III digest (8-587 bp) Plasmid pBR322 (4361 bp)	View Download
Proteins in human serum	BioPro IEX QA	IgG Transferrin Albumin	View Download
Proteins	BioPro IEX QA	Ovalbumin Trypsin inhibitor (soybean)	View Download
Synthetic oligonucleotides (Single-strand DNA)	BioPro IEX QF	5’-TCATCACACTGAATACCAAT-3’ (20 mer) 5’-GTCATCACACTGAATACCAAT-3’ (21 mer)	View Download
Synthetic oligonucleotides (Single-strand RNA)	BioPro IEX QF	5’-U(M)C(M)A(M)U(M)C(M)A(M)C(M)A(M)C(M) U(M)G(M)A(M)A(M)U(M)A(M)C(M)C(M)A(M)A(M)U(M)-3’ (20 mer) 5’-G(M)U(M)C(M)A(M)U(M)C(M)A(M)C(M)A(M)C(M)U(M) G(M)A(M)A(M)U(M)A(M)C(M)C(M)A(M)A(M)U(M)-3’ (21 mer)	View Download

Specifications

	BioPro IEX QF YMC Accura BioPro IEX QF	BioPro IEX SF YMC Accura BioPro IEX SF	BioPro IEX QA	BioPro IEX SP
Base Material	hydrophilic polymer (polymethacrylate)
Particle Size [µm]	3, 5 µm		5 µm
Pore Size [nm]	non-porous		100
pH range	2.0 - 12.0
Column hardware	PEEK bioinert coated stainless steel (YMC Accura)		PEEK
Temperature range	4 - 60°C (PEEK) 4 - 80°C (YMC Accura)		4 - 60°C

Phase Details

BioPro IEX QF/SF / YMC Accura BioPro IEX QF/SF

(formerly)	BioPro IEX QF YMC Accura BioPro IEX QF	BioPro IEX SF YMC Accura BioPro IEX SF
Matrix	Hydrophilic polymer (polymethacrylate)
Particle Size	3, 5 µm
Pore Size	Non-porous
Charged group	-CH₂N⁺(CH₃)₃ (strong anion exchanger)	-(CH₂)₃SO₃^- (strong cation exchanger)
Counter ion	Cl^-	Na⁺
Ion-exchange capacity	0.075-0.110 meq/ml-resin	0.230-0.290 meq/ml-resin
Dynamic binding capacity	12-20 mg BSA/ml-resin	10-18 mg human IgG/ml-resin
pH range	2.0 - 12.0
Column hardware	PEEK bioinert coated stainless steel (YMC Accura)
Temperature range	4 - 60°C (PEEK) 4 - 80°C (YMC Accura)
Pressure limit	3 µm: 25 MPa (3,625 psi, PEEK); 15 - 20 MPa (2,175 - 2,900 psi, YMC Accura) 5 µm: 6 - 12 MPa (870 - 1,740 psi, PEEK); 10 - 30 MPa (1,450 - 4,350 psi, YMC Accura)

The non-porous BioPro IEX particles offer high efficiency, exceptional resolution, and low operating pressures.

BioPro IEX QA/SP

(formerly)	BioPro IEX QA	BioPro IEX SP
Matrix	Hydrophilic polymer (polymethacrylate)
Particle Size	5 µm
Pore Size	100 nm / 1000 Å
Charged group	-CH₂N⁺(CH₃)₃ (strong anion exchanger)	-(CH₂)₃SO₃^- (strong cation exchanger)
Counter ion	Cl^-	Na⁺
Ion-exchange capacity	0.075-0.100 meq/ml-resin	0.070-0.095 meq/ml-resin
Dynamic binding capacity	110-150 mg BSA/ml-resin	70-100 mg human IgG/ml-resin
pH range	2.0 - 12.0
Temperature range	4 - 60°C
Column hardware	PEEK
Pressure limit	2.5 - 3.5 MPa (360 - 510 psi)

The porous type of BioPro IEX shows great adsorption capacity and excellent recovery, and is especially suitable for semipreparative separations of proteins and antibodies. Compared with conventional porous polymer anion-exchange columns, BioPro IEX phases give higher dynamic binding capacities (DBC) and recovery rates. This indicates that BioPro IEX has a much lower nonspecific adsorption compared to conventional columns.

Technical Library

Title	Document Type	Language	Download
(U)HPLC analysis of siRNA under denaturing and non-denaturing conditions using IP-RP and AEX	Application Note	EN	Download
Analysis of mRNA coupled to enhanced green fluorescent protein by RP and IEX	Application Note	EN	Download
Charge heterogeneity characterisation of an IgG4-based mAb using AEX coupled to MS	Application Note	EN	Download
HPLC Analysis of Oligonucleotides with BioPro IEX QF	Application Note	EN	Download
High resolution analysis of monoclonal antibodies using the cation exchange column BioPro IEX SF	Application Note	EN	Download
MS compatible charge variant analysis of 28 commercial MAbs by CEX	Application Note	EN	Download
Native AEX-MS analysis of IgG4-based mAbs	Application Note	EN	Download
Native IEX-MS analysis of monoclonal antibodies using YMC Accura BioPro IEX SF columns	Application Note	EN	Download
Native online SCX-MS analysis of monoclonal antibodies using YMC's BioPro IEX SF	Application Note	EN	Download
Optimisation of oligonucleotide separations using YMC´s non porous anion exchanger BioPro IEX QF	Application Note	EN	Download
Optimisation of phosphorothioate oligonucleotide separation using BioPro IEX QF	Application Note	EN	Download
YMC Application Collection - Antibodies & ADCs	Application Note	EN	Download
YMC Oligonucleotide Columns	Brochure	EN	Download
BioPro IEX Columns Care and Use	Care and Use Instructions	EN	Download
YMC Biochromatography Columns	Catalogue	EN	Download
YMC Biochromatography Columns (HQ)	Catalogue	EN	Download
YMC Biochromatography Columns - IEX Columns Chapter	Catalogue	EN	Download
Minimising carryover in oligonucleotide analysis by AEX	Expert Tip	EN	Download
Analytical Stationary Phases for BioLC from YMC	Overview	EN	Download
YMC Alternatives to Existing BioLC Columns	Overview	EN	Download
A Stability-Indicating Method for Separation of Charge Variant Impurities of a PEGylated Protein by CEX-HPLC	Poster	EN	Download
An Evaluation of YMC’s Strong Cation-Exchange Stationary Phase for the Analysis of Charged Isoforms of a Commercially Available Antibody Drug	Poster	EN	Download
Oligonucleotide purification using anion exchange (AEX) and ion-pair reversed phase chromatography (IP-RP LC)	Poster	EN	Download
Optimisation of oligonucleotide separations on ion exchange chromatography	Poster	EN	Download
The Principles of BioLC & YMC BioLC Phase Overview	Poster	EN	Download
Use of Small Particle YMC SEC and IEX Materials for Improved Characterization of Monoclonal Antibodies	Poster	EN	Download
Advantages of strong cation exchangers vs. weak cation exchangers	Technical Note	EN	Download
Characterisation of charge heterogeneity of therapeutic monoclonal antibodies via IEX-MS using BioPro IEX SF from YMC	Technical Note	EN	Download
High temperature stability of BioPro IEX columns	Technical Note	EN	Download
How to optimise your oligonucleotide analysis	Technical Note	EN	Download
Utilisation of AEX-MS for charge variant analysis of IgG4-based mAbs!	Technical Note	EN	Download
The Utility of a Cation Exchange Column for Successful Charge-Variant Analysis of PEGylated Proteins	Whitepaper	EN	Download

Ordering Information

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2-3 days
1-2 weeks
3-4 weeks

Product	Description	Price	Stock
QAA0S05-0346WP	BioPro IEX QA, PEEK HPLC column (4.6 mm ID), 100 nm, S-5 µm, 30 x 4.6 mm	- 2054 CHF 1675 €
QAA0S05-0546WP	BioPro IEX QA, PEEK HPLC column (4.6 mm ID), 100 nm, S-5 µm, 50 x 4.6 mm	- 2054 CHF 1675 €
QAA0S05-1046WP	BioPro IEX QA, PEEK HPLC column (4.6 mm ID), 100 nm, S-5 µm, 100 x 4.6 mm	- 2213 CHF 1805 €
QF00S03-0346WP	BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 30 x 4.6 mm	- 1465 CHF 1195 €
QF00S03-03Q1PTC	YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-3 µm, 30 x 2.1 mm	- 2148 CHF 1752 €
QF00S03-0546PTC	YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-3 µm, 50 x 4.6 mm	- 2148 CHF 1752 €
QF00S03-0546WP	BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 50 x 4.6 mm	- 1758 CHF 1434 €
QF00S03-05Q1PTC	YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-3 µm, 50 x 2.1 mm	- 2148 CHF 1752 €
QF00S03-1046PTC	YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-3 µm, 100 x 4.6 mm	- 2375 CHF 1937 €
QF00S03-1046WP	BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 100 x 4.6 mm	- 1979 CHF 1614 €
QF00S03-10Q1PTC	YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-3 µm, 100 x 2.1 mm	- 2375 CHF 1937 €
QF00S03-1546PTC	YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-3 µm, 150 x 4.6 mm	- 2941 CHF 2399 €
QF00S03-1546WP	BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 150 x 4.6 mm	- 2398 CHF 1956 €
QF00S03-15Q1PTC	YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-3 µm, 150 x 2.1 mm	- 2941 CHF 2399 €
QF00S05-0346WP	BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 30 x 4.6 mm	- 1708 CHF 1393 €
QF00S05-03Q1PTC	YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-5 µm, 30 x 2.1 mm	- 1998 CHF 1630 €
QF00S05-0546PTC	YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-5 µm, 50 x 4.6 mm	- 1998 CHF 1630 €
QF00S05-0546WP	BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 50 x 4.6 mm	- 1998 CHF 1630 €
QF00S05-05Q1PTC	YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-5 µm, 50 x 2.1 mm	- 1998 CHF 1630 €
QF00S05-1002WT	BioPro IEX QF, non-porous, narrowbore HPLC column (2.0 mm ID), S-5 µm, 100 x 2.0 mm	- 2969 CHF 2422 €
QF00S05-1046PTC	YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-5 µm, 100 x 4.6 mm	- 2267 CHF 1849 €
QF00S05-1046WP	BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 100 x 4.6 mm	- 2267 CHF 1849 €
QF00S05-10Q1PTC	YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-5 µm, 100 x 2.1 mm	- 2267 CHF 1849 €
QF00S05-1546PTC	YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-5 µm, 150 x 4.6 mm	- 2924 CHF 2385 €
QF00S05-1546WP	BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 150 x 4.6 mm	- 2924 CHF 2385 €
QF00S05-15Q1PTC	YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-5 µm, 150 x 2.1 mm	- 2924 CHF 2385 €
QF00S05-2546PTC	YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-5 µm, 250 x 4.6 mm	- 3245 CHF 2647 €
QF00S06-1010WT	BioPro IEX QF, non-porous, semi-preparative HPLC column (10 mm ID), S-6 µm, 100 x 10 mm	- 14831 CHF 12097 €
QF00S06-1020WT	BioPro IEX QF, non-porous, semi-preparative HPLC column (20 mm ID), S-6 µm, 100 x 20 mm	- 29704 CHF 24228 €
QF00S06-1030WT	BioPro IEX QF, non-porous, semi-preparative HPLC column (30 mm ID), S-6 µm, 100 x 30 mm	- 59575 CHF 48593 €
QF00S06-2546WP	BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-6 µm, 250 x 4.6 mm	- 3400 CHF 2773 €
SF00S03-0346WP	BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 30 x 4.6 mm	- 1465 CHF 1195 €
SF00S03-03Q1PTC	YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-3 µm, 30 x 2.1 mm	- 2148 CHF 1752 €
SF00S03-0546PTC	YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-3 µm, 50 x 4.6 mm	- 2148 CHF 1752 €
SF00S03-0546WP	BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 50 x 4.6 mm	- 1758 CHF 1434 €
SF00S03-05Q1PTC	YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-3 µm, 50 x 2.1 mm	- 2148 CHF 1752 €
SF00S03-1046PTC	YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-3 µm, 100 x 4.6 mm	- 2375 CHF 1937 €
SF00S03-1046WP	BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 100 x 4.6 mm	- 1979 CHF 1614 €
SF00S03-10Q1PTC	YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-3 µm, 100 x 2.1 mm	- 2375 CHF 1937 €
SF00S03-1546PTC	YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-3 µm, 150 x 4.6 mm	- 2941 CHF 2399 €
SF00S03-1546WP	BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 150 x 4.6 mm	- 2398 CHF 1956 €
SF00S03-15Q1PTC	YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-3 µm, 150 x 2.1 mm	- 2941 CHF 2399 €
SF00S05-0346WP	BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 30 x 4.6 mm	- 1708 CHF 1393 €
SF00S05-03Q1PTC	YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-5 µm, 30 x 2.1 mm	- 1998 CHF 1630 €
SF00S05-0502WT	BioPro IEX SF, non-porous, narrowbore HPLC column (2.0 mm ID), S-5 µm, 50 x 2.0 mm	- 2598 CHF 2119 €
SF00S05-0546PTC	YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-5 µm, 50 x 4.6 mm	- 1998 CHF 1630 €
SF00S05-0546WP	BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 50 x 4.6 mm	- 1998 CHF 1630 €
SF00S05-05Q1PTC	YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-5 µm, 50 x 2.1 mm	- 1998 CHF 1630 €
SF00S05-1002WT	BioPro IEX SF, non-porous, narrowbore HPLC column (2.0 mm ID), S-5 µm, 100 x 2.0 mm	- 2969 CHF 2422 €
SF00S05-1008WT	BioPro IEX SF, non-porous, classical analytical HPLC column (8.0 mm ID), S-5 µm, 100 x 8.0 mm	- 6527 CHF 5324 €
SF00S05-1046PTC	YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-5 µm, 100 x 4.6 mm	- 2267 CHF 1849 €
SF00S05-1046WP	BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 100 x 4.6 mm	- 2267 CHF 1849 €
SF00S05-10Q1PTC	YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-5 µm, 100 x 2.1 mm	- 2267 CHF 1849 €
SF00S05-1546PTC	YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-5 µm, 150 x 4.6 mm	- 2924 CHF 2385 €
SF00S05-1546WP	BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 150 x 4.6 mm	- 2924 CHF 2385 €
SF00S05-15Q1PTC	YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-5 µm, 150 x 2.1 mm	- 2924 CHF 2385 €
SF00S05-2546PTC	YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-5 µm, 250 x 4.6 mm	- 3245 CHF 2647 €
SF00S06-1010WT	BioPro IEX SF, non-porous, semi-preparative HPLC column (10 mm ID), S-6 µm, 100 x 10 mm	- 14831 CHF 12097 €
SF00S06-1020WT	BioPro IEX SF, non-porous, semi-preparative HPLC column (20 mm ID), S-6 µm, 100 x 20 mm	- 29704 CHF 24228 €
SF00S06-1030WT	BioPro IEX SF, non-porous, semi-preparative HPLC column (30 mm ID), S-6 µm, 100 x 30 mm	- 59575 CHF 48593 €
SF00S06-2546WP	BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-6 µm, 250 x 4.6 mm	- 3400 CHF 2773 €
SPA0S05-0346WP	BioPro IEX SP, PEEK HPLC column (4.6 mm ID), 100 nm, S-5 µm, 30 x 4.6 mm	- 2054 CHF 1675 €
SPA0S05-0546WP	BioPro IEX SP, PEEK HPLC column (4.6 mm ID), 100 nm, S-5 µm, 50 x 4.6 mm	- 2054 CHF 1675 €
SPA0S05-1008WT	BioPro IEX SP, classical analytical HPLC column (8.0 mm ID), S-5 µm, 250 x 8.0 mm	- 7183 CHF 5859 €
SPA0S05-1046WP	BioPro IEX SP, PEEK HPLC column (4.6 mm ID), 100 nm, S-5 µm, 100 x 4.6 mm	- 2213 CHF 1805 €
XRPRCP02	Precolumn filter 2 µm for 1/16" OD + holder, PEEK (350 bar)	- 353 CHF 288 €
XRPRCP25	Replacement precolumn filter 2 µm for 1/16" OD, PEEK (350 bar)	- 257 CHF 210 €

Ion Exchange Columns

For High Resolution Applications

BioPro IEX: Ion Exchange Columns

Available phases are:

BioPro Ion Exchange Columns are characterised by:

Applications

Specifications

Phase Details

Technical Library

Ordering Information

Expertise portal: More expert content for you

Online presentations: Experience us live

Downloads: Our expert library

Contact

Ion Exchange Columns

For High Resolution Applications

BioPro IEX: Ion Exchange Columns

Available phases are:

BioPro Ion Exchange Columns are characterised by:

Applications

Specifications

Phase Details

Technical Library

Ordering Information

Related Categories

Expertise Topics

Expertise portal: More expert content for you

Online presentations: Experience us live

Downloads: Our expert library

Contact