Available phases are:
BioPro Ion Exchange Columns are characterised by:
- Non-porous or porous
particles - High loadability and
recovery - Very high resolution
- High dynamic binding capacity
- Long term stability
- Excellent lot-to-lot
reproducibility
Applications
Application | Column | Compound name | |
DNA fragments | BioPro IEX QF | DNA fragments | |
Lambda DNA Hind Ⅲ/EcoRⅠ restriction fragments | BioPro IEX QF | Lambda DNA Hind III/EcoR I digest (130-23, 130 bp) | |
Monoclonal antibody (MAb) | BioPro IEX SF | Humanized monoclonal IgG1 | |
Peptide mapping | BioPro IEX QA | Peptide mapping | |
Plasmid pBR322 and pBR322 Hae III restriction fragments | BioPro IEX QF | Plasmid pBR322 Hae III digest (8-587 bp) Plasmid pBR322 (4361 bp) | |
Proteins in human serum | BioPro IEX QA | IgG Transferrin Albumin | |
Proteins | BioPro IEX QA | Ovalbumin Trypsin inhibitor (soybean) | |
Synthetic oligonucleotides (Single-strand DNA) | BioPro IEX QF | 5’-TCATCACACTGAATACCAAT-3’ (20 mer) 5’-GTCATCACACTGAATACCAAT-3’ (21 mer) | |
Synthetic oligonucleotides (Single-strand RNA) | BioPro IEX QF | 5’-U(M)C(M)A(M)U(M)C(M)A(M)C(M)A(M)C(M) U(M)G(M)A(M)A(M)U(M)A(M)C(M)C(M)A(M)A(M)U(M)-3’ (20 mer) 5’-G(M)U(M)C(M)A(M)U(M)C(M)A(M)C(M)A(M)C(M)U(M) G(M)A(M)A(M)U(M)A(M)C(M)C(M)A(M)A(M)U(M)-3’ (21 mer) |
Specifications
|
BioPro IEX QF
YMC Accura BioPro IEX QF
|
BioPro IEX SF
YMC Accura BioPro IEX SF
|
BioPro IEX QA
|
BioPro IEX SP
|
||
Base Material
|
hydrophilic polymer (polymethacrylate)
|
|||||
Particle Size [µm]
|
3, 5 µm
|
5 µm
|
||||
Pore Size [nm]
|
non-porous
|
100
|
||||
pH range
|
2.0 - 12.0
|
|||||
Column hardware
|
PEEK
bioinert coated stainless steel (YMC Accura)
|
PEEK
|
||||
Temperature range
|
4 - 60°C (PEEK)
4 - 80°C (YMC Accura)
|
4 - 60°C
|
Phase Details
BioPro IEX QF/SF / YMC Accura BioPro IEX QF/SF
(formerly)
|
BioPro IEX QF
YMC Accura BioPro IEX QF
|
BioPro IEX SF
YMC Accura BioPro IEX SF
|
Matrix
|
Hydrophilic polymer (polymethacrylate)
|
|
Particle Size
|
3, 5 µm
|
|
Pore Size
|
Non-porous
|
|
Charged group
|
-CH2N+(CH3)3
(strong anion exchanger)
|
-(CH2)3SO3-
(strong cation exchanger)
|
Counter ion
|
Cl-
|
Na+
|
Ion-exchange capacity
|
0.075-0.110 meq/ml-resin
|
0.230-0.290 meq/ml-resin
|
Dynamic binding capacity
|
12-20 mg BSA/ml-resin
|
10-18 mg human IgG/ml-resin
|
pH range
|
2.0 - 12.0
|
|
Column hardware
|
PEEK
bioinert coated stainless steel (YMC Accura)
|
|
Temperature range
|
4 - 60°C (PEEK)
4 - 80°C (YMC Accura)
|
|
Pressure limit
|
3 µm: 25 MPa (3,625 psi, PEEK); 15 - 20 MPa (2,175 - 2,900 psi, YMC Accura)
5 µm: 6 - 12 MPa (870 - 1,740 psi, PEEK); 10 - 30 MPa (1,450 - 4,350 psi, YMC Accura)
|
The non-porous BioPro IEX particles offer high efficiency, exceptional resolution, and low operating pressures.
BioPro IEX QA/SP
(formerly)
|
BioPro IEX QA
|
BioPro IEX SP
|
||
Matrix
|
Hydrophilic polymer (polymethacrylate)
|
|||
Particle Size
|
5 µm
|
|||
Pore Size
|
100 nm / 1000 Å
|
|||
Charged group
|
-CH2N+(CH3)3
(strong anion exchanger)
|
-(CH2)3SO3-
(strong cation exchanger)
|
||
Counter ion
|
Cl-
|
Na+
|
||
Ion-exchange capacity
|
0.075-0.100 meq/ml-resin
|
0.070-0.095 meq/ml-resin
|
||
Dynamic binding capacity
|
110-150 mg BSA/ml-resin
|
70-100 mg human IgG/ml-resin
|
||
pH range
|
2.0 - 12.0
|
|||
Temperature range
|
4 - 60°C
|
|||
Column hardware
|
PEEK
|
|||
Pressure limit
|
2.5 - 3.5 MPa (360 - 510 psi)
|
Technical Library
Title | Document Type | Language | Download |
(U)HPLC analysis of siRNA under denaturing and non-denaturing conditions using IP-RP and AEX | Application Note | EN | |
Analysis of mRNA coupled to enhanced green fluorescent protein by RP and IEX | Application Note | EN | |
Charge heterogeneity characterisation of an IgG4-based mAb using AEX coupled to MS | Application Note | EN | |
HPLC Analysis of Oligonucleotides with BioPro IEX QF | Application Note | EN | |
High resolution analysis of monoclonal antibodies using the cation exchange column BioPro IEX SF | Application Note | EN | |
MS compatible charge variant analysis of 28 commercial MAbs by CEX | Application Note | EN | |
Native AEX-MS analysis of IgG4-based mAbs | Application Note | EN | |
Native IEX-MS analysis of monoclonal antibodies using YMC Accura BioPro IEX SF columns | Application Note | EN | |
Native online SCX-MS analysis of monoclonal antibodies using YMC's BioPro IEX SF | Application Note | EN | |
Optimisation of oligonucleotide separations using YMC´s non porous anion exchanger BioPro IEX QF | Application Note | EN | |
Optimisation of phosphorothioate oligonucleotide separation using BioPro IEX QF | Application Note | EN | |
YMC Application Collection - Antibodies & ADCs | Application Note | EN | |
YMC Oligonucleotide Columns | Brochure | EN | |
BioPro IEX Columns Care and Use | Care and Use Instructions | EN | |
YMC Biochromatography Columns | Catalogue | EN | |
YMC Biochromatography Columns (HQ) | Catalogue | EN | |
YMC Biochromatography Columns - IEX Columns Chapter | Catalogue | EN | |
Minimising carryover in oligonucleotide analysis by AEX | Expert Tip | EN | |
Analytical Stationary Phases for BioLC from YMC | Overview | EN | |
YMC Alternatives to Existing BioLC Columns | Overview | EN | |
A Stability-Indicating Method for Separation of Charge Variant Impurities of a PEGylated Protein by CEX-HPLC | Poster | EN | |
An Evaluation of YMC’s Strong Cation-Exchange Stationary Phase for the Analysis of Charged Isoforms of a Commercially Available Antibody Drug | Poster | EN | |
Oligonucleotide purification using anion exchange (AEX) and ion-pair reversed phase chromatography (IP-RP LC) | Poster | EN | |
Optimisation of oligonucleotide separations on ion exchange chromatography | Poster | EN | |
The Principles of BioLC & YMC BioLC Phase Overview | Poster | EN | |
Use of Small Particle YMC SEC and IEX Materials for Improved Characterization of Monoclonal Antibodies | Poster | EN | |
Advantages of strong cation exchangers vs. weak cation exchangers | Technical Note | EN | |
Characterisation of charge heterogeneity of therapeutic monoclonal antibodies via IEX-MS using BioPro IEX SF from YMC | Technical Note | EN | |
High temperature stability of BioPro IEX columns | Technical Note | EN | |
How to optimise your oligonucleotide analysis | Technical Note | EN | |
Utilisation of AEX-MS for charge variant analysis of IgG4-based mAbs! | Technical Note | EN | |
The Utility of a Cation Exchange Column for Successful Charge-Variant Analysis of PEGylated Proteins | Whitepaper | EN |
Ordering Information
Please login to see the prices.
- 2-3 days
- 1-2 weeks
- 3-4 weeks
Product | Description | Price | Stock |
QAA0S05-0346WP | BioPro IEX QA, PEEK HPLC column (4.6 mm ID), 100 nm, S-5 µm, 30 x 4.6 mm | - 2117 CHF 1727 € | |
QAA0S05-0546WP | BioPro IEX QA, PEEK HPLC column (4.6 mm ID), 100 nm, S-5 µm, 50 x 4.6 mm | - 2117 CHF 1727 € | |
QAA0S05-1046WP | BioPro IEX QA, PEEK HPLC column (4.6 mm ID), 100 nm, S-5 µm, 100 x 4.6 mm | - 2282 CHF 1861 € | |
QF00S03-0346WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 30 x 4.6 mm | - 1510 CHF 1232 € | |
QF00S03-03Q1PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-3 µm, 30 x 2.1 mm | - 2214 CHF 1806 € | |
QF00S03-0546PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-3 µm, 50 x 4.6 mm | - 2214 CHF 1806 € | |
QF00S03-0546WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 50 x 4.6 mm | - 1812 CHF 1478 € | |
QF00S03-05Q1PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-3 µm, 50 x 2.1 mm | - 2214 CHF 1806 € | |
QF00S03-1046PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-3 µm, 100 x 4.6 mm | - 2448 CHF 1997 € | |
QF00S03-1046WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 100 x 4.6 mm | - 2040 CHF 1664 € | |
QF00S03-10Q1PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-3 µm, 100 x 2.1 mm | - 2448 CHF 1997 € | |
QF00S03-1546PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-3 µm, 150 x 4.6 mm | - 3032 CHF 2473 € | |
QF00S03-1546WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 150 x 4.6 mm | - 2473 CHF 2017 € | |
QF00S03-15Q1PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-3 µm, 150 x 2.1 mm | - 3032 CHF 2473 € | |
QF00S05-0346WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 30 x 4.6 mm | - 1761 CHF 1436 € | |
QF00S05-03Q1PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-5 µm, 30 x 2.1 mm | - 2061 CHF 1681 € | |
QF00S05-0546PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-5 µm, 50 x 4.6 mm | - 2061 CHF 1681 € | |
QF00S05-0546WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 50 x 4.6 mm | - 2061 CHF 1681 € | |
QF00S05-05Q1PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-5 µm, 50 x 2.1 mm | - 2061 CHF 1681 € | |
QF00S05-1002WT | BioPro IEX QF, non-porous, narrowbore HPLC column (2.0 mm ID), S-5 µm, 100 x 2.0 mm | - 3061 CHF 2497 € | |
QF00S05-1046PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-5 µm, 100 x 4.6 mm | - 2337 CHF 1906 € | |
QF00S05-1046WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 100 x 4.6 mm | - 2337 CHF 1906 € | |
QF00S05-10Q1PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-5 µm, 100 x 2.1 mm | - 2337 CHF 1906 € | |
QF00S05-1546PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-5 µm, 150 x 4.6 mm | - 3015 CHF 2459 € | |
QF00S05-1546WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 150 x 4.6 mm | - 3015 CHF 2459 € | |
QF00S05-15Q1PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-5 µm, 150 x 2.1 mm | - 3015 CHF 2459 € | |
QF00S05-2546PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-5 µm, 250 x 4.6 mm | - 3346 CHF 2729 € | |
QF00S06-1010WT | BioPro IEX QF, non-porous, semi-preparative HPLC column (10 mm ID), S-6 µm, 100 x 10 mm | - 15291 CHF 12472 € | |
QF00S06-1020WT | BioPro IEX QF, non-porous, semi-preparative HPLC column (20 mm ID), S-6 µm, 100 x 20 mm | - 30624 CHF 24979 € | |
QF00S06-1030WT | BioPro IEX QF, non-porous, semi-preparative HPLC column (30 mm ID), S-6 µm, 100 x 30 mm | - 61421 CHF 50099 € | |
QF00S06-2546WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-6 µm, 250 x 4.6 mm | - 3505 CHF 2859 € | |
SF00S03-0346WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 30 x 4.6 mm | - 1510 CHF 1232 € | |
SF00S03-03Q1PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-3 µm, 30 x 2.1 mm | - 2214 CHF 1806 € | |
SF00S03-0546PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-3 µm, 50 x 4.6 mm | - 2214 CHF 1806 € | |
SF00S03-0546WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 50 x 4.6 mm | - 1812 CHF 1478 € | |
SF00S03-05Q1PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-3 µm, 50 x 2.1 mm | - 2214 CHF 1806 € | |
SF00S03-1046PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-3 µm, 100 x 4.6 mm | - 2448 CHF 1997 € | |
SF00S03-1046WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 100 x 4.6 mm | - 2040 CHF 1664 € | |
SF00S03-10Q1PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-3 µm, 100 x 2.1 mm | - 2448 CHF 1997 € | |
SF00S03-1546PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-3 µm, 150 x 4.6 mm | - 3032 CHF 2473 € | |
SF00S03-1546WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 150 x 4.6 mm | - 2473 CHF 2017 € | |
SF00S03-15Q1PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-3 µm, 150 x 2.1 mm | - 3032 CHF 2473 € | |
SF00S05-0346WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 30 x 4.6 mm | - 1761 CHF 1436 € | |
SF00S05-03Q1PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-5 µm, 30 x 2.1 mm | - 2061 CHF 1681 € | |
SF00S05-0502WT | BioPro IEX SF, non-porous, narrowbore HPLC column (2.0 mm ID), S-5 µm, 50 x 2.0 mm | - 2679 CHF 2185 € | |
SF00S05-0546PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-5 µm, 50 x 4.6 mm | - 2061 CHF 1681 € | |
SF00S05-0546WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 50 x 4.6 mm | - 2061 CHF 1681 € | |
SF00S05-05Q1PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-5 µm, 50 x 2.1 mm | - 2061 CHF 1681 € | |
SF00S05-1002WT | BioPro IEX SF, non-porous, narrowbore HPLC column (2.0 mm ID), S-5 µm, 100 x 2.0 mm | - 3061 CHF 2497 € | |
SF00S05-1008WT | BioPro IEX SF, non-porous, classical analytical HPLC column (8.0 mm ID), S-5 µm, 100 x 8.0 mm | - 6730 CHF 5489 € | |
SF00S05-1046PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-5 µm, 100 x 4.6 mm | - 2337 CHF 1906 € | |
SF00S05-1046WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 100 x 4.6 mm | - 2337 CHF 1906 € | |
SF00S05-10Q1PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-5 µm, 100 x 2.1 mm | - 2337 CHF 1906 € | |
SF00S05-1546PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-5 µm, 150 x 4.6 mm | - 3015 CHF 2459 € | |
SF00S05-1546WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 150 x 4.6 mm | - 3015 CHF 2459 € | |
SF00S05-15Q1PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-5 µm, 150 x 2.1 mm | - 3015 CHF 2459 € | |
SF00S05-2546PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-5 µm, 250 x 4.6 mm | - 3346 CHF 2729 € | |
SF00S06-1010WT | BioPro IEX SF, non-porous, semi-preparative HPLC column (10 mm ID), S-6 µm, 100 x 10 mm | - 15291 CHF 12472 € | |
SF00S06-1020WT | BioPro IEX SF, non-porous, semi-preparative HPLC column (20 mm ID), S-6 µm, 100 x 20 mm | - 30624 CHF 24979 € | |
SF00S06-1030WT | BioPro IEX SF, non-porous, semi-preparative HPLC column (30 mm ID), S-6 µm, 100 x 30 mm | - 61421 CHF 50099 € | |
SF00S06-2546WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-6 µm, 250 x 4.6 mm | - 3505 CHF 2859 € | |
SPA0S05-0346WP | BioPro IEX SP, PEEK HPLC column (4.6 mm ID), 100 nm, S-5 µm, 30 x 4.6 mm | - 2117 CHF 1727 € | |
SPA0S05-0546WP | BioPro IEX SP, PEEK HPLC column (4.6 mm ID), 100 nm, S-5 µm, 50 x 4.6 mm | - 2117 CHF 1727 € | |
SPA0S05-1008WT | BioPro IEX SP, classical analytical HPLC column (8.0 mm ID), S-5 µm, 250 x 8.0 mm | - 7406 CHF 6041 € | |
SPA0S05-1046WP | BioPro IEX SP, PEEK HPLC column (4.6 mm ID), 100 nm, S-5 µm, 100 x 4.6 mm | - 2282 CHF 1861 € | |
XRPRCP02 | Precolumn filter 2 µm for 1/16" OD + holder, PEEK (350 bar) | - 370 CHF 302 € | |
XRPRCP25 | Replacement precolumn filter 2 µm for 1/16" OD, PEEK (350 bar) | - 271 CHF 221 € |