Normal Phase Stationary Phases
Due to the highly sophisticated production process YMC’s highly spherical silica material shows premium performance and great lot-to-lot reproducibility. The reason for this can be summarised in two main qualities: the very narrow physical and chemical product specifications and the outstanding purity. For normal phase chromatography, YMC offers both unmodified silica and silica modified with polar groups. The silica-based phases have proven successful in a variety of large-scale industrial processes.
Particle sizes
10 - 15 - 20 - 50 - 75 - 150 μm
Pore sizes
6 - 8 - 12 - 20 - 30 nm
Modifications
Amino - Cyano - Diol - unmodified
Advantages
- Higher sample load
- Less backpressure
- Longer column usage
- More repacking times possible
- More efficient column packing
Available Stationary Phases:
| YMC*Gel NH2-HG (Amino) | YMC*Gel CN-HG (Cyano) | YMC*Gel Diol-HG | YMC*Gel SIL-HG (Silica) | YMC*Gel SIL (Silica) | |
| Base Material | high grade silica | classical silica | |||
| Modification | NH2 | CN | Diol | unmodified | unmodified |
| Particle Size [µm] | 10, 15, 20, 50 | 10, 15, 20, 50 | 10, 15, 20, 50 | 10, 15, 20, 50 | 50, 75, 100, 150 |
| Pore Size [nm] | 12, (20, 30) | 12, (20, 30) | 12, 20, 30 | 6, 8, 12, 20, 30 | 6, 12 |
| Surface Area [m²/g] | 330, (175, 100) | 330, (175, 100) | 330, 175, 100 | 650, 510, 330, 175, 100 | 700, 400 |
Compared to conventional silica, YMC's high-grade silica has more uniform particles and a narrower pore size distribution. This leads to reduced back pressure and improved chromatographic efficiency. In addition, high-grade silica has fewer so-called "balloon particles". These can easily break during the packing process and the fragments lead to an increase in back pressure as they can easily settle into the free spaces between the particles. This results in clogging of the column.
| Pore Size (nm) | Surface Area (m²/g) | Recommendation |
| 12 | 330 | Suitable for the majority of separations For most organic compounds Higher surface area and sample loading than 20 nm and 30 nm |
| 20 | 175 | For molecules from 10 kDa to 50 kDa For bulky organic compounds Higher surface area and higher sample loading than 30 nm |
| 30 | 100 | For molecules larger than 40 kDa For organic compounds with excessive retention on smaller pore size |
The use of spherical stationary phases increases the economics of the purification process:
- The column is packed only once: labor cost is reduced
- The efficiency of the spherical support is much higher: higher loadability
- The material can be cleaned in the column: the column can be used more than 30 times
In cooperation with a pharmaceutical company, a comprehensive comparison was done with irregular and spherical silica for the separation of dimethyl phthalate and diethyl phthalate.
| Title | Document Type | Language | Download |
 Purification of Carotenoids via NP |
Application Note | EN | |
 YMC*Gel HG Packing Materials |
Brochure | EN | |
 YMC*Gel SIL-HG Care and Use |
Care and Use Instructions | EN | |
 YMC Bulk Packing Materials for RP-, NP- and Chiral Prep. LC |
Overview | EN | |
 Linear Scale-Up in Preparative LC Method Development |
Technical Note | EN | |
| Polar Bonded Phases: Alternatives to Silica for NP Separations |
Technical Note | EN |
Please contact YMC for pricing or specific solutions for your purification process.
Julia Bartman
Product Manager Bulk Media
ph. +49 2064 427-260
Particle Size/Pore Size | 12 nm | 20 nm | 30 nm |
| 10 µm | NHG12S11 | NHG20S11 | NHG30S11 |
| 15 µm | NHG12S16 | NHG20S16 | NHG30S16 |
| 20 µm | NHG12S21 | NHG20S21 | NHG30S21 |
| 50 µm | NHG12S50 |
Typical pack sizes:
Laboratory scale: smallest amount is 100 grams up to 4 kg PE bottle
Industrial scale: more than 4 kg in double lined PE bags inside metal drums (10 or 25 kg drums)
Particle Size/Pore Size | 12 nm | 20 nm | 30 nm |
| 10 µm | CNG12S11 | CNG20S11 | CNG30S11 |
| 15 µm | CNG12S16 | CNG20S16 | CNG30S16 |
| 20 µm | CNG12S21 | CNG20S21 | CNG30S21 |
| 50 µm | CNG12S50 |
Typical pack sizes:
Laboratory scale: smallest amount is 100 grams up to 4 kg PE bottle
Industrial scale: more than 4 kg in double lined PE bags inside metal drums (10 or 25 kg drums)
Particle Size/Pore Size | 12 nm | 20 nm | 30 nm |
| 10 µm | DLG12S11 | DLG20S11 | DLG30S11 |
| 15 µm | DLG12S16 | DLG20S16 | DLG30S16 |
| 20 µm | DLG12S21 | DLG20S21 | DLG30S21 |
| 50 µm | DLG12S50 |
Typical pack sizes:
Laboratory scale: smallest amount is 100 grams up to 4 kg PE bottle
Industrial scale: more than 4 kg in double lined PE bags inside metal drums (10 or 25 kg drums)
Particle Size/Pore Size | 6 nm | 8 nm | 12 nm | 20 nm | 30 nm |
| 10 µm | SLG06S11 | SLG08S11 | SLG12S11 | SLG20S11 | SLG30S11 |
| 15 µm | SLG06S16 | SLG08S16 | SLG12S16 | SLG20S16 | SLG30S16 |
| 20 µm | SLG06S21 | SLG08S21 | SLG12S21 | SLG20S21 | SLG30S21 |
| 50 µm | SLG12S50 | ||||
| 75 µm | SL06S75 | SL12S75 | |||
| 150 µm | SL06SA5 | SL12SA5 |
Typical pack sizes:
Laboratory scale: smallest amount is 100 grams up to 4 kg PE bottle
Industrial scale: more than 4 kg in double lined PE bags inside metal drums (10 or 25 kg drums)
