One of the advantages of YMC’s BioPro IEX resins is its full scalability. This property provides an identical chromatographic behaviour across all particle sizes. This is beneficial, as separation and fractionation can be optimised on the analytical scale. Depending on the required resolution the optimal bead size can be selected. Then, the process can be reliably transferred to the production scale. This enables highly flexible implementation of BioPro IEX resins, resulting in a perfectly tailored solution.

Cleaning-in-place (CIP) is essential for the economic use of packed chromatography columns. Efficient cleaning procedures increase the lifetime of the separation process and thereby contribute to the overall cost effectiveness. Furthermore, powerful CIP procedures strongly increase the safety and productivity of every downstream process.

IEX resins from YMC are fully compatible to typical CIP procedures. As an example, CIP studies have been performed using NaOH solutions with BioPro IEX S75/IEX Q75 as well as with BioPro IEX SmartSep S30/Q30. All YMC IEX resins maintain their performance values even after 100 CIP cycles.

The pressure flow characteristics of IEX resins are mainly based on the physical properties of the base beads used for the resin. In general, the particle size of the resin is the overriding parameter responsible for the separation efficiency according to the diffusion processes and for the inherent backpressures during the chromatographic processes.

In the first example the pressure flow characteristics of BioPro IEX Q75 from YMC and Q Sepharose FF from Cytiva are compared. The YMC resin shows much better pressure flow characteristics. This may be related to the rigidity of the base beads used. As a result, the YMC resin can be used over a greater flow rate range and with higher flow rates. For a pressure range of up to 3 bar, the YMC resin can be used with flow rates of up to 800 cm/hr for a bed length of 24 cm.