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Expert Topics
Guide to Biochromatography Columns for HPLC & UHPLC
What Is Biochromatography?
Biochromatography is a specialized field of HPLC & UHPLC designed for the separation, purification, and analysis of biomolecules such as proteins, peptides, nucleotides, and antibodies. Unlike conventional chromatography, biochromatography columns are optimized to maintain sample integrity, high binding capacity, and reproducible separations. YMC offers a comprehensive range of BioLC Columns, covering multiple chromatographic techniques to suit different biomolecule characteristics.
Types of BioLC Columns and Their Applications
Ion Exchange (IEX) Columns: Used in IEX chromatography for charge-based separation of biomolecules. Ideal for protein purification, nucleic acid separations, and antibody analysis.
Size Exclusion (SEC) Columns: Fractionate molecules by molecular weight, widely applied for antibody aggregation studies and biopharmaceutical characterization.
RP Bioseparation Columns: Specialized RP Bio Columns for hydrophobic interactions, crucial for peptide and protein separations in biochromatography.
Hydrophobic Interaction (HIC) Columns: Separate antibodies, protein conjugates, and ADCs based on hydrophobicity, ensuring high recovery and resolution.
Bioinert Columns: Prevent metal interactions for sensitive biomolecules, including phosphopeptides, oligonucleotides, and phospholipids.
Advantages of YMC BioLC Columns
High resolution & peak symmetry: Achieve optimal separation of complex biomolecules.
Scalability: Fully compatible with both analytical and preparative HPLC & UHPLC applications.
Wide range of pore sizes & selectivities: Tailored solutions for various molecular weights and hydrophobic properties.
Optimized for biopharmaceutical applications: Designed for antibody analysis, protein purification, and proteomics workflows.
Choosing the Right BioLC Column
Selecting the right BioLC column depends on the properties of the biomolecules being analyzed. Ion Exchange Chromatography (IEX) is ideal for separating charged molecules like proteins and nucleic acids using anionic or cationic exchange phases. Size Exclusion Chromatography (SEC) columns separate biomolecules based on molecular size, making them essential for antibody aggregate analysis and protein fractionation.
For hydrophobic biomolecules, Reversed Phase Bioseparation (RP Bio) Columns provide high-resolution separations, while Hydrophobic Interaction Chromatography (HIC) Columns offer a gentler approach for protein complexes and antibody-drug conjugates (ADCs). Bioinert-coated columns prevent metal interactions, ensuring high recovery of sensitive compounds like phosphopeptides and oligonucleotides.
With its broad selection, YMC provides optimized solutions for bioanalytical workflows in pharmaceutical research, biopharmaceutical development, and proteomics.
Find Your Ideal BioLC Column
YMC’s biochromatography columns ensure high efficiency, robust performance, and superior biomolecule recovery in HPLC & UHPLC applications. Whether you are working in pharmaceutical research, biopharmaceutical development, or proteomics, YMC provides tailored solutions for your bioanalytical workflows.
Need help selecting the right column? Contact YMC Europe for expert consultation.