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Use our Application Search to discover chromatography solutions for HPLC and UHPLC. Filter by industry, compound, or column type to find the ideal match for your analysis.
Advantages
YMC Size Exclusion Columns
High flexibility
Full scalability
Excellent reproducibility
High stability
Cost-efficiency
Precise molecular weight determination
No secondary interactions
Wide molecular weight range
CUSTOMER TESTIMONIALS
What Our Customers Say
About the Product
“The YMC-Pack Diol SEC column has been successfully used, also for subsequent method validation.”
Rubén Pedrosa Segon, Quality Control Pharmaceutical Department, OFICE S.L. (ES)
PRODUCT MEDIA
Engineered for
Molecular Clarity
Downloads
YMC SEC Columns Brochure
Download our detailed product brochure for in-depth insights into YMC Biochromatography columns or access the complete media package with extended materials.
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Applications
| Application | Column | Compound name | |
| Casein | YMC-Pack Diol-300 | Casein | |
| Chondroitin sulfate A | YMC-Pack Diol-300 | Chondroitin sulfate A, Na salt | |
| Chondroitin sulfate B | YMC-Pack Diol-300 | Chondroitin sulfate B, Na salt | |
| Chondroitin sulfate C | YMC-Pack Diol-300 | Chondroitin sulfate C, Na salt | |
| Chondroitin | YMC-Pack Diol-300 | Chondroitin, Na salt | |
| Cyclodextrins | YMC-Pack Diol-60 | γ-Cyclodextrin β-Cyclodextrin α-Cyclodextrin | |
| Cytochrome c | YMC-Pack Diol-120 | Cytochrome c (hexamer) Cytochrome c (tetramer) Cytochrome c (trimer) Cytochrome c (dimer) Cytochrome c (monomer) | |
| Glycine oligomers | YMC-Pack Diol-60 | Pentaglycine Triglycine Glycine | |
| Heparan sulfate | YMC-Pack Diol-300 | Heparan sulfate, Na salt | |
| Heparin | YMC-Pack Diol-300 | Heparin, Na salt | |
| Human IgG (λ), myeloma protein | YMC-Pack Diol-300 | Human IgG (λ), myeloma protein | |
| Human IgM (κ), myeloma protein | YMC-Pack Diol-300 | Normal human IgM (κ), myeloma protein | |
| Human immunoglobulins | YMC-Pack Diol-200 | Human IgM (κ), myeloma protein Normal human IgA Normal human IgG | |
| Hyaluronic acid | YMC-Pack Diol-300 | Hyaluronic acid, Na salt | |
| Keratan polysulfate | YMC-Pack Diol-300 | Keratan polysulfate, Na salt | |
| Keratan sulfate | YMC-Pack Diol-300 | Keratan sulfate, Na salt | |
| Maltooligosaccharides (G1 ～ G15) | YMC-Pack Diol-120 | Maltopentadecaose (G15) Maltotridecaose (G13) Maltoundecaose (G11) Maltoheptaose (G7) Maltopentaose (G5) Maltotriose (G3) Glucose (G1) | |
| Monoclonal antibody (MAb) | YMC-Pack Diol-200 | Mouse monoclonal IgG1 (anti-human IgG4) | |
| Nonionic surfactants | YMC-Pack Diol-120 | Triton X-100 | |
| Normal human IgA | YMC-Pack Diol-300 | Normal human IgA | |
| Normal human IgG | YMC-Pack Diol-300 | Normal human IgG | |
| Peptide fragments from myoglobin | YMC-Pack Diol-120 | myoglobin fragments 1-153 (MW 17000) 1-131 (MW 14500) 56-153 (MW 10700) 56-131 (MW 8200) 1-55 (MW 6300) 132-153 (MW 2500) | |
| Peptide mapping | YMC-Pack Diol-60 | Peptide mapping | |
| Peptides | YMC-Pack Diol-60 | Insulin (Bovine) Neurotensin Angiotensin II Glycine | |
| Plasma constituents | YMC-Pack Diol-200 | Human serumIgM IgA Fibrinogen γ-Globulin IgG Transferrin Albumin α1-Antitrypsin Carbonic anhydrase Lysozyme Uric acid Creatinine | |
| Plasmid pBR322 and pBR322 Hae III restriction fragments | YMC-Pack Diol-200 | Plasmid pBR322 (4361 bp) Plasmid pBR322 Hae III digest (8-587 bp) | |
| Plasmid pBR322 restriction fragments | YMC-Pack Diol-200 | Plasmid pBR322 cleaved with restriction endonuclease Msp I | |
| Polyethyleneglycols | YMC-Pack Diol-120 | PEG #20000 (MW 20000) PEG #6000 (MW 8400) PEG #4000 (MW 3350) PEG #1000 (MW 1000) PEG #300 (MW 300) Ethyleneglycol (MW 62) | |
| Proteins for molecular weight marker | YMC-Pack Diol-200 | Glutamate dehydrogenase (MW 290000) Lactate dehydrogenase (MW 142000) Enolase (MW 67000) Adenylate kinase (MW 32000) Cytochrome c (MW 12400) | |
| Proteins in human serum | YMC-Pack Diol-200 | IgG Transferrin Albumin | |
| Proteins in mouse ascites fluid | YMC-Pack Diol-200 | IgG Transferrin Albumin | |
| Pullulans | YMC-Pack Diol-300 | Pullulan P-800 (MW 853000) Pullulan P-50 (MW 48000) Pullulan P-20 (MW 23700) Pullulan P-5 (MW 5800) | |
| lgG, Fab and Fc fragments | YMC-Pack Diol-200 | Rabbit lgG Mouse lgG Fc fragment Mouse lgG Fab fragment |
Specifications
|
|
YMC-SEC MAB
|
YMC-Pack
Diol-60
|
YMC-Pack
Diol-120
|
YMC-Pack
Diol-200
|
YMC-Pack
Diol-300
|
|
Base material
|
silica
|
||||
|
Suitable for
|
antibodies, fragments, aggregates
|
peptides,
carbohydrates
|
intermediate
proteins
|
large proteins,
antibody fragments
|
very large proteins,
antibody aggregates
|
|
Rec. MW Range
|
10 - 700 kDa
|
<10 kDa
|
5 - 100 kDa
|
5 - 300 kDa
|
20 - 1,000 kDa
|
|
Pore Size [nm]
|
25
|
6
|
12
|
20
|
30
|
|
Particle Size [µm]
|
3
|
3, 5
|
3, 5
|
2, 3, 5
|
2, 3, 5
|
|
Rec. pH Range
|
|
5.0 - 7.5
|
|||
|
Max. Temp.
|
|
40°C
|
|||
|
Bioinert column option
|
-
|
||||
Phase Details
YMC-SEC MAB
|
Modification (USP class)
|
Dihydroxypropyl (USP L20/L59)
|
|
Suitable for
|
antibodies, antibody fragments, antibody aggregates
|
|
Recommended MW range
|
10 - 700 kDa
|
|
Pore Size
|
25 nm / 250 Å
|
|
Particle Size
|
3 µm
|
|
Rec. pH Range
|
5.0 - 7.5
|
|
Max. Temp.
|
40°C
|
|
Pressure Limit
|
14 MPa / 2,030 psi
|
YMC-Pack Diol-60
|
Modification (USP class)
|
Dihydroxypropyl (USP L20)
|
|
Suitable for
|
peptides, carbohydrates
|
|
Recommended MW range
|
<10 kDa
|
|
Pore Size
|
6 nm / 60 Å
|
|
Particle Size
|
3 µm, 5 µm
|
|
Rec. pH Range
|
5.0 - 7.5
|
|
Max. Temp.
|
40°C
|
|
Pressure Limit
|
20 MPa / 3,000 psi
|
YMC-Pack Diol-120
|
Modification (USP class)
|
Dihydroxypropyl (USP L20/L59)
|
|
Suitable for
|
intermediate proteins
|
|
Recommended MW range
|
5 - 100 kDa
|
|
Pore Size
|
12 nm / 120 Å
|
|
Particle Size
|
3 µm, 5 µm
|
|
Rec. pH Range
|
5.0 - 7.5
|
|
Max. Temp.
|
40°C
|
|
Pressure Limit
|
20 MPa / 3,000 psi
|
YMC-Pack Diol-200
|
Modification (USP class)
|
Dihydroxypropyl (USP L20/L33/L59)
|
|
Suitable for
|
large proteins, antibody fragments
|
|
Recommended MW range
|
5 - 300 kDa
|
|
Pore Size
|
20 nm / 200 Å
|
|
Particle Size
|
2 µm, 3 µm, 5 µm
|
|
Rec. pH Range
|
5.0 - 7.5
|
|
Max. Temp.
|
40°C
|
|
Pressure Limit
|
2 µm: 45 MPa / 6,525 psi
3/5 µm: 20 MPa / 3,000 psi
|
YMC-Pack Diol-300
|
Modification (USP class)
|
Dihydroxypropyl (USP L20/L33/L59)
|
|
Suitable for
|
very large proteins, antibody aggregates
|
|
Recommended MW range
|
20 - 1,000 kDa
|
|
Pore Size
|
30 nm / 300 Å
|
|
Particle Size
|
2 µm, 3 µm, 5 µm
|
|
Rec. pH Range
|
5.0 - 7.5
|
|
Max. Temp.
|
40°C
|
|
Pressure Limit
|
2 µm: 45 MPa / 6,525 psi
3/5 µm: 20 MPa / 3,000 psi
|
Technical Library
| Title | Document Type | Language | Download |
 Analysis of Bevacizumab (Avastin®) by SEC-MALLS |
Application Note | EN | |
| Efficient separation and analysis of antibody fragments and their drug conjugates |
Application Note | EN | |
| Maximising the separation capacity in SEC by coupling two YMC-Pack Diol columns |
Application Note | EN | |
| Pore size selection for separation of oligonucleotides by YMC-Pack Diol columns |
Application Note | EN | |
| SEC Analysis of MAb Fragments with Increased Throughput and High Resolution |
Application Note | EN | |
| SEC separation of the ADC Brentuximab vedotin from its aggregates and fragments |
Application Note | EN | |
| SEC-Analysis of Bevacizumab (Avastin®) and its aggregates and fragments |
Application Note | EN | |
| Separation of siRNA and its single strands by SEC using YMC-Pack Diol-120 |
Application Note | EN | |
| YMC Application Collection - Antibodies & ADCs |
Application Note | EN | |
 YMC-Pack Diol SEC (HPLC/UHPLC) Care and Use |
Care and Use Instructions | EN | |
| YMC-SEC MAB Care and Use |
Care and Use Instructions | EN | |
 YMC Biochromatography Columns |
Catalogue | EN | |
| YMC Biochromatography Columns - SEC Columns Chapter |
Catalogue | EN | |
 How to calculate the molecular weight of nucleic acids |
Expert Tip | EN | |
 Analytical Stationary Phases for BioLC from YMC |
Overview | EN | |
| YMC Alternatives to Existing BioLC Columns |
Overview | EN | |
 The Principles of BioLC & YMC BioLC Phase Overview |
Poster | EN | |
| Use of Small Particle YMC SEC and IEX Materials for Improved Characterization of Monoclonal Antibodies |
Poster | EN | |
 YMC SEC columns and light scattering detection |
Technical Note | EN | |
 Grundlagen der Chromatographie für die Analyse und Aufreinigung von Biologika |
Whitepaper | DE |
Ordering Information
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- 2-3 days
- 1-2 weeks
- 3-4 weeks
| Product | Description | Price | Stock |
| DL06S03-0104GC | YMC-Pack Diol-60, analytical SEC guard cartridges (4.0 mm ID), 6 nm, S-3µm, 10 x 4.0 mm | - 650 CHF 530 € | |
| DL06S03-1508WT | YMC-Pack Diol-200, SEC column (8.0 mm ID), 6 nm, S-3 µm, 150 x 8.0 mm | - 1750 CHF 1427 € | |
| DL06S03-1546WT | YMC-Pack Diol-60, SEC column (4.6 mm ID), 6 nm, S-3 µm, 150 x 4.6 mm | - 1254 CHF 1023 € | |
| DL06S03-2546WT | YMC-Pack Diol-60, SEC column (4.6 mm ID), 6 nm, S-3 µm, 250 x 4.6 mm | - 1399 CHF 1141 € | |
| DL06S03-3006WT | YMC-Pack Diol-60, SEC column (6.0 mm ID), 6 nm, S-3 µm, 300 x 6.0 mm | - 1732 CHF 1413 € | |
| DL06S03-3008WT | YMC-Pack Diol-60, SEC column (8.0 mm ID), 6 nm, S-3 µm, 300 x 8.0 mm | - 2122 CHF 1731 € | |
| DL06S03-3046WT | YMC-Pack Diol-60, SEC column (4.6 mm ID), 6 nm, S-3 µm, 300 x 4.6 mm | - 1566 CHF 1277 € | |
| DL06S05-0110CC | YMC-Pack Diol-60, semi-preparative SEC guard cartridges (10 mm ID), 6 nm, S-5 µm, 10 x 10 mm | - 646 CHF 527 € | |
| DL06S05-0308WTG | YMC-Pack Diol-60, SEC guard column (8.0 mm ID), 6 nm, S-5 µm, 30 x 8.0 mm | - 1133 CHF 924 € | |
| DL06S05-0310WTG | YMC-Pack Diol-60, semi-preparative SEC guard column (8.0 mm ID), 6 nm, S-5 µm, 30 x 10 mm | - 1133 CHF 924 € | |
| DL06S05-1546WT | YMC-Pack Diol-60, SEC column (4.6 mm ID), 6 nm, S-5 µm, 150 x 4.6 mm | - 1133 CHF 924 € | |
| DL06S05-2506WT | YMC-Pack Diol-60, SEC column (6.0 mm ID), 6 nm, S-5 µm, 250 x 6.0 mm | - 1460 CHF 1191 € | |
| DL06S05-2510WT | YMC-Pack Diol-60, semi-preparative SEC column (10 mm ID), 6 nm, S-5 µm, 250 x 10 mm | - 2079 CHF 1696 € | |
| DL06S05-2520WT | YMC-Pack Diol-60, semi-preparative SEC column (20 mm ID), 6 nm, S-5 µm, 250 x 20 mm | - 6383 CHF 5206 € | |
| DL06S05-2530WT | YMC-Pack Diol-60, semi-preparative SEC column (30 mm ID), 6 nm, S-5 µm, 250 x 30 mm | - 17848 CHF 14558 € | |
| DL06S05-2546WT | YMC-Pack Diol-60, SEC column (4.6 mm ID), 6 nm, S-5 µm, 250 x 4.6 mm | - 1298 CHF 1059 € | |
| DL06S05-3006WT | YMC-Pack Diol-60, SEC column (6.0 mm ID), 6 nm, S-5 µm, 300 x 6.0 mm | - 1566 CHF 1277 € | |
| DL06S05-3008WT | YMC-Pack Diol-60, SEC column (8.0 mm ID), 6 nm, S-5 µm, 300 x 8.0 mm | - 1933 CHF 1577 € | |
| DL06S05-3020WT | YMC-Pack Diol-60, semi-preparative SEC column (20 mm ID), 6 nm, S-5 µm, 300 x 20 mm | - 6758 CHF 5512 € | |
| DL06S05-3046WT | YMC-Pack Diol-60, SEC column (4.6 mm ID), 6 nm, S-5 µm, 300 x 4.6 mm | - 1399 CHF 1141 € | |
| DL06S05-5008WT | YMC-Pack Diol-60, SEC column (8.0 mm ID), 6 nm, S-5 µm, 500 x 8.0 mm | - 3213 CHF 2621 € | |
| DL12S03-0104GC | YMC-Pack Diol-120, analytical SEC guard cartridges (4.0 mm ID), 12 nm, S-3µm, 10 x 4.0 mm | - 650 CHF 530 € | |
| DL12S03-0308WTG | YMC-Pack Diol-120, SEC guard column (8.0 mm ID), 12 nm, S-3 µm, 30 x 8.0 mm | - 1133 CHF 924 € | |
| DL12S03-1546PTP | YMC-Pack Diol-120, metal-free PEEK-lined SEC column (4.6 mm ID), 12 nm, S-3 µm, 150 x 4.6 mm | - 3004 CHF 2450 € | |
| DL12S03-1546WT | YMC-Pack Diol-120, SEC column (4.6 mm ID), 12 nm, S-3 µm, 150 x 4.6 mm | - 1254 CHF 1023 € | |
| DL12S03-2546WT | YMC-Pack Diol-120, SEC column (4.6 mm ID), 12 nm, S-3 µm, 250 x 4.6 mm | - 1399 CHF 1141 € | |
| DL12S03-3006WT | YMC-Pack Diol-120, SEC column (6.0 mm ID), 12 nm, S-3 µm, 300 x 6.0 mm | - 1732 CHF 1413 € | |
| DL12S03-3008WT | YMC-Pack Diol-120, SEC column (8.0 mm ID), 12 nm, S-3 µm, 300 x 8.0 mm | - 2122 CHF 1731 € | |
| DL12S03-3046WT | YMC-Pack Diol-120, SEC column (4.6 mm ID), 12 nm, S-3 µm, 300 x 4.6 mm | - 1566 CHF 1277 € | |
| DL12S05-0104GC | YMC-Pack Diol-120, analytical SEC guard cartridges (4.0 mm ID), 12 nm, S-5µm, 10 x 4.0 mm | - 650 CHF 530 € | |
| DL12S05-0110CC | YMC-Pack Diol-120, semi-preparative SEC guard cartridges (10 mm ID), 12 nm, S-5µm, 10 x 10 mm | - 666 CHF 543 € | |
| DL12S05-0120CCN | YMC-Pack Diol-120, semi-preparative SEC guard cartridges (20 mm ID), 12 nm, S-5µm, 10 x 20 mm | - 992 CHF 809 € | |
| DL12S05-01Q1GC | YMC-Pack Diol-120, analytical SEC guard cartridges (2.1 mm ID), 12 nm, S-5µm, 10 x 2.1 mm | - 650 CHF 530 € | |
| DL12S05-0308WTG | YMC-Pack Diol-120, SEC guard column (8.0 mm ID), 12 nm, S-5 µm, 30 x 8.0 mm | - 1133 CHF 924 € | |
| DL12S05-0310WTG | YMC-Pack Diol-120, semi-preparative SEC guard column (8.0 mm ID), 12 nm, S-5 µm, 30 x 10 mm | - 1133 CHF 924 € | |
| DL12S05-1520WT | YMC-Pack Diol-120, semi-preparative SEC column (20 mm ID), 12 nm, S-5 µm, 150 x 20 mm | - 5119 CHF 4175 € | |
| DL12S05-1546WT | YMC-Pack Diol-120, analytical SEC column (4.6 mm ID), 12 nm, S-5µm, 150 x 4.6 mm | - 1133 CHF 924 € | |
| DL12S05-2504WT | YMC-Pack Diol-120, analytical SEC column (4.0 mm ID), 12 nm, S-5µm, 250 x 4.0 mm | - 1298 CHF 1059 € | |
| DL12S05-2506WT | YMC-Pack Diol-120, SEC column (6.0 mm ID), 12 nm, S-5 µm, 250 x 6.0 mm | - 1460 CHF 1191 € | |
| DL12S05-2510WT | YMC-Pack Diol-120, semi-preparative SEC column (10 mm ID), 12 nm, S-5 µm, 250 x 10 mm | - 2079 CHF 1696 € | |
| DL12S05-2520WT | YMC-Pack Diol-120, semi-preparative SEC column (20 mm ID), 12 nm, S-5 µm, 250 x 20 mm | - 6383 CHF 5206 € | |
| DL12S05-2530WT | YMC-Pack Diol-120, semi-preparative SEC column (30 mm ID), 12 nm, S-5 µm, 250 x 30 mm | - 17848 CHF 14558 € | |
| DL12S05-2546WT | YMC-Pack Diol-120, SEC column (4.6 mm ID), 12 nm, S-5 µm, 250 x 4.6 mm | - 1298 CHF 1059 € | |
| DL12S05-3006WT | YMC-Pack Diol-120, SEC column (6.0 mm ID), 12 nm, S-5 µm, 300 x 6.0 mm | - 1566 CHF 1277 € | |
| DL12S05-3008WT | YMC-Pack Diol-120, SEC column (8.0 mm ID), 12 nm, S-5 µm, 300 x 8.0 mm | - 1933 CHF 1577 € | |
| DL12S05-3020WT | YMC-Pack Diol-120, semi-preparative SEC column (20 mm ID), 12 nm, S-5 µm, 300 x 20 mm | - 6758 CHF 5512 € | |
| DL12S05-3046WT | YMC-Pack Diol-120, SEC column (4.6 mm ID), 12 nm, S-5 µm, 300 x 4.6 mm | - 1399 CHF 1141 € | |
| DL12S05-5008WT | YMC-Pack Diol-120, SEC column (8.0 mm ID), 12 nm, S-5 µm, 500 x 8.0 mm | - 3213 CHF 2621 € | |
| DL20S02-0104GC | YMC-Pack Diol-200 UHPLC, analytical SEC guard cartridges (4.0 mm ID), 20 nm, S-2µm, 10 x 4.0 mm | - 1075 CHF 877 € | |
| DL20S02-0204GC | YMC-Pack Diol-200 UHPLC, analytical SEC guard cartridges (4.0 mm ID), 20 nm, S-2µm, 20 x 4.0 mm | - 1230 CHF 1003 € | |
| DL20S02-1546WT | YMC-Pack Diol-200 UHPLC, SEC column (4.6 mm ID), 20 nm, S-2 µm, 150 x 4.6 mm | - 2707 CHF 2208 € | |
| DL20S02-3046WT | YMC-Pack Diol-200 UHPLC, SEC column (4.6 mm ID), 20 nm, S-2 µm, 300 x 4.6 mm | - 3645 CHF 2973 € | |
| DL20S03-0104GC | YMC-Pack Diol-200, analytical SEC guard cartridges (4.0 mm ID), 20 nm, S-3µm, 10 x 4.0 mm | - 650 CHF 530 € | |
| DL20S03-0308WTG | YMC-Pack Diol-200, SEC guard column (8.0 mm ID), 20 nm, S-3 µm, 30 x 8.0 mm | - 1133 CHF 924 € | |
| DL20S03-0546WT | YMC-Pack Diol-200, SEC column (4.6 mm ID), 20 nm, S-3 µm, 50 x 4.6 mm | - 1046 CHF 853 € | |
| DL20S03-1508WT | YMC-Pack Diol-200, SEC column (8.0 mm ID), 20 nm, S-3 µm, 150 x 8.0 mm | - 1750 CHF 1427 € | |
| DL20S03-1546WT | YMC-Pack Diol-200, SEC column (4.6 mm ID), 20 nm, S-3 µm, 150 x 4.6 mm | - 1254 CHF 1023 € | |
| DL20S03-2546WT | YMC-Pack Diol-200, SEC column (4.6 mm ID), 20 nm, S-3 µm, 250 x 4.6 mm | - 1399 CHF 1141 € | |
| DL20S03-3006WT | YMC-Pack Diol-200, SEC column (6.0 mm ID), 20 nm, S-3 µm, 300 x 6.0 mm | - 1732 CHF 1413 € | |
| DL20S03-3008WT | YMC-Pack Diol-200, SEC column (8.0 mm ID), 20 nm, S-3 µm, 300 x 8.0 mm | - 2122 CHF 1731 € | |
| DL20S03-3046WT | YMC-Pack Diol-200, SEC column (4.6 mm ID), 20 nm, S-3 µm, 300 x 4.6 mm | - 1566 CHF 1277 € | |
| DL20S05-0104GC | YMC-Pack Diol-200, analytical SEC guard cartridges (4.0 mm ID), 20 nm, S-5µm, 10 x 4.0 mm | - 650 CHF 530 € | |
| DL20S05-0105WFG | YMC-Pack Diol-200, SEC guard SEC column (5.0 mm ID), 12 nm, S-5 µm, 10 x 5.0 mm (1pc.) | - 726 CHF 592 € | |
| DL20S05-0110CC | YMC-Pack Diol-200, semi-preparative SEC guard cartridges (10 mm ID), 20 nm, S-5µm, 10 x 10 mm | - 666 CHF 543 € | |
| DL20S05-0120CCN | YMC-Pack Diol-200, semi-preparative SEC guard cartridges (20 mm ID), 20 nm, S-5µm, 10 x 20 mm | - 992 CHF 809 € | |
| DL20S05-0308WTG | YMC-Pack Diol-200, SEC guard column ((8.0 mm ID)), 20 nm, S-5 µm, 30 x 8.0 mm | - 1064 CHF 868 € | |
| DL20S05-1002WT | YMC-Pack Diol-200, analytical SEC column (2.0 mm ID), 20 nm, S-5µm, 100 x 2.0 mm | - 962 CHF 785 € | |
| DL20S05-1508WT | YMC-Pack Diol-200, SEC column (8.0 mm ID), 20 nm, S-5 µm, 150 x 8.0 mm | - 1411 CHF 1151 € | |
| DL20S05-2506WT | YMC-Pack Diol-200, SEC column (6.0 mm ID), 20 nm, S-5 µm, 250 x 6.0 mm | - 1372 CHF 1119 € | |
| DL20S05-2510WT | YMC-Pack Diol-200, semi-preparative SEC column (10 mm ID), 20 nm, S-5 µm, 250 x 10 mm | - 1952 CHF 1592 € | |
| DL20S05-2520WT | YMC-Pack Diol-200, semi-preparative SEC column (20 mm ID), 20 nm, S-5 µm, 250 x 20 mm | - 5994 CHF 4889 € | |
| DL20S05-2530WT | YMC-Pack Diol-200, semi-preparative SEC column (30 mm ID), 20 nm, S-5 µm, 250 x 30 mm | - 16757 CHF 13668 € | |
| DL20S05-2546WT | YMC-Pack Diol-200, SEC column (4.6 mm ID), 20 nm, S-5 µm, 250 x 4.6 mm | - 1220 CHF 995 € | |
| DL20S05-3006WT | YMC-Pack Diol-200, SEC column (6.0 mm ID), 20 nm, S-5 µm, 300 x 6.0 mm | - 1470 CHF 1199 € | |
| DL20S05-3008WT | YMC-Pack Diol-200, SEC column (8.0 mm ID), 20 nm, S-5 µm, 300 x 8.0 mm | - 1816 CHF 1481 € | |
| DL20S05-3046WT | YMC-Pack Diol-200, SEC column (4.6 mm ID), 20 nm, S-5 µm, 300 x 4.6 mm | - 1313 CHF 1071 € | |
| DL20S05-5008WT | YMC-Pack Diol-200, SEC column (8.0 mm ID), 20 nm, S-5 µm, 500 x 8.0 mm | - 3018 CHF 2462 € | |
| DL30S02-0104GC | YMC-Pack Diol-300 UHPLC, analytical SEC guard cartridges (4.0 mm ID), 30 nm, S-2µm, 10 x 4.0 mm | - 1075 CHF 877 € | |
| DL30S02-1546WT | YMC-Pack Diol-300 UHPLC, SEC column (4.6 mm ID), 30 nm, S-2 µm, 150 x 4.6 mm | - 2707 CHF 2208 € | |
| DL30S02-15H0AU | YMC-Pack Diol-300 UHPLC, capillary SEC column 1/16", 30 nm, S-2 µm, 150 x 0.3 mm | - 1340 CHF 1093 € | |
| DL30S02-3046WT | YMC-Pack Diol-300 UHPLC, SEC column (4.6 mm ID), 30 nm, S-2 µm, 300 x 4.6 mm | - 3645 CHF 2973 € | |
| DL30S03-0104GC | YMC-Pack Diol-300, analytical SEC guard cartridges (4.0 mm ID), 30 nm, S-3µm, 10 x 4.0 mm | - 650 CHF 530 € | |
| DL30S03-0308WTG | YMC-Pack Diol-300, SEC guard column (8.0 mm ID), 30 nm, S-3 µm, 30 x 8.0 mm | - 1133 CHF 924 € | |
| DL30S03-1508WT | YMC-Pack Diol-300, SEC column (8.0 mm ID), 30 nm, S-3 µm, 150 x 8.0 mm | - 1750 CHF 1427 € | |
| DL30S03-1546WT | YMC-Pack Diol-300, SEC column (4.6 mm ID), 30 nm, S-3 µm, 150 x 4.6 mm | - 1254 CHF 1023 € | |
| DL30S03-2546WT | YMC-Pack Diol-300, SEC column (4.6 mm ID), 30 nm, S-3 µm, 250 x 4.6 mm | - 1399 CHF 1141 € | |
| DL30S03-3006WT | YMC-Pack Diol-300, SEC column (6.0 mm ID), 30 nm, S-3 µm, 300 x 6.0 mm | - 1732 CHF 1413 € | |
| DL30S03-3008WT | YMC-Pack Diol-300, SEC column (8.0 mm ID), 30 nm, S-3 µm, 300 x 8.0 mm | - 2122 CHF 1731 € | |
| DL30S03-3046WT | YMC-Pack Diol-300, SEC column (4.6 mm ID), 30 nm, S-3 µm, 300 x 4.6 mm | - 1566 CHF 1277 € | |
| DL30S05-0104GC | YMC-Pack Diol-300, analytical SEC guard cartridges (4.0 mm ID), 30 nm, S-5µm, 10 x 4.0 mm | - 650 CHF 530 € | |
| DL30S05-0110CC | YMC-Pack Diol-300, semi-preparative SEC guard cartridges (10 mm ID),30 nm, S-5µm, 10 x 10 mm | - 666 CHF 543 € | |
| DL30S05-0120CCN | YMC-Pack Diol-300, semi-preparative SEC guard cartridges (20 mm ID), 30 nm, S-5µm, 10 x 20 mm | - 992 CHF 809 € | |
| DL30S05-0204GC | YMC-Pack Diol-300, analytical SEC guard cartridges (4.0 mm ID), 30 nm, S-5µm, 20 x 4.0 mm | - 745 CHF 608 € | |
| DL30S05-0308WTG | YMC-Pack Diol-300, SEC guard column (8.0 mm ID), 30 nm, S-5 µm, 30 x 8.0 mm | - 949 CHF 774 € | |
| DL30S05-0310WTG | YMC-Pack Diol-300, semi-preparative SEC guard column (10 mm ID), 30 nm, S-5 µm, 30 x 10 mm | - 1064 CHF 868 € | |
| DL30S05-1508WT | YMC-Pack Diol-300, SEC column (8.0 mm ID), 30 nm, S-3 µm, 150 x 8.0 mm | - 1411 CHF 1151 € | |
| DL30S05-1520WT | YMC-Pack Diol-300, semi-preparative SEC column (20 mm ID), 30 nm, S-5 µm, 150 x 20 mm | - 4807 CHF 3921 € | |
| DL30S05-1546WT | YMC-Pack Diol-300, SEC column (4.6 mm ID), 30 nm, S-5 µm, 150 x 4.6 mm | - 1064 CHF 868 € | |
| DL30S05-2506WT | YMC-Pack Diol-300, SEC column (6.0 mm ID), 30 nm, S-5 µm, 250 x 6.0 mm | - 1372 CHF 1119 € | |
| DL30S05-2510WT | YMC-Pack Diol-300, semi-preparative SEC column (10 mm ID), 30 nm, S-5 µm, 250 x 10 mm | - 1952 CHF 1592 € | |
| DL30S05-2520WT | YMC-Pack Diol-300, semi-preparative SEC column (20 mm ID), 30 nm, S-5 µm, 250 x 20 mm | - 5994 CHF 4889 € | |
| DL30S05-2530WT | YMC-Pack Diol-300, semi-preparative SEC column (30 mm ID), 30 nm, S-5 µm, 250 x 30 mm | - 16757 CHF 13668 € | |
| DL30S05-2546WT | YMC-Pack Diol-300, SEC column (4.6 mm ID), 30 nm, S-5 µm, 250 x 4.6 mm | - 1220 CHF 995 € | |
| DL30S05-3006WT | YMC-Pack Diol-300, SEC column (6.0 mm ID), 30 nm, S-5 µm, 300 x 6.0 mm | - 1470 CHF 1199 € | |
| DL30S05-3008WT | YMC-Pack Diol-300, SEC column (8.0 mm ID), 30 nm, S-5 µm, 300 x 8.0 mm | - 1816 CHF 1481 € | |
| DL30S05-3020WT | YMC-Pack Diol-300, semi-preparative SEC column (20 mm ID), 30 nm, S-5 µm, 300 x 20 mm | - 6346 CHF 5176 € | |
| DL30S05-3046WT | YMC-Pack Diol-300, SEC column (4.6 mm ID), 30 nm, S-5 µm, 300 x 4.6 mm | - 1313 CHF 1071 € | |
| DL30S05-5008WT | YMC-Pack Diol-300, SEC column (8.0 mm ID), 30 nm, S-5 µm, 500 x 8.0 mm | - 3018 CHF 2462 € | |
| DLM25S03-0104GC | YMC-SEC MAB, analytical guard cartridges (4.0 mm ID), 25 nm, S-3µm, 10 x 4.0 mm | - 650 CHF 530 € | |
| DLM25S03-0308WTG | YMC-SEC MAB, SEC guard column (8.0 mm ID), 25 nm, S-3 µm, 30 x 8.0 mm | - 1311 CHF 1069 € | |
| DLM25S03-15E8AU | YMC-SEC MAB, capillary column 1/16", 25 nm, S-3 µm, 150 x 0.075 mm | - 1315 CHF 1073 € | |
| DLM25S03-15H0AU | YMC-SEC MAB, capillary column 1/16", 25 nm, S-3 µm, 150 x 0.3 mm | - 1315 CHF 1073 € | |
| DLM25S03-15J0AU | YMC-SEC MAB, capillary column 1/16", 25 nm, S-3 µm, 150 x 0.5 mm | - 1315 CHF 1073 € | |
| DLM25S03-25H0AU | YMC-SEC MAB, capillary column 1/16", 25 nm, S-3 µm, 250 x 0.3 mm | - 1315 CHF 1073 € | |
| DLM25S03-25J0AU | YMC-SEC MAB, capillary column 1/16", 25 nm, S-3 µm, 250 x 0.5 mm | - 1315 CHF 1073 € | |
| DLM25S03-3008WT | YMC-SEC MAB, SEC column (8.0 mm ID), 25 nm, S-3 µm, 300 x 8.0 mm | - 4867 CHF 3970 € | |
| DLM25S03-3046WT | YMC-SEC MAB, SEC column (4.6 mm ID), 25 nm, S-3 µm, 300 x 4.6 mm | - 3254 CHF 2654 € | |
| DLM25S03-30J0AU | YMC-SEC MAB, capillary column 1/16", 25 nm, S-3 µm, 300 x 0.5 mm | - 1315 CHF 1073 € | |
| DLM25S03-E5H0AU | YMC-SEC MAB, capillary guard column 1/16", 25 nm, S-3 µm, 5 x 0.3 mm | - 696 CHF 568 € | |
| XRPRCP02 | Precolumn filter 2 µm for 1/16" OD + holder, PEEK (350 bar) | - 383 CHF 312 € | |
| XRPRCP25 | Replacement precolumn filter 2 µm for 1/16" OD, PEEK (350 bar) | - 281 CHF 229 € |
Guide to Size Exclusion Chromatography (SEC) for HPLC & UHPLC
What Are SEC Columns?
Size Exclusion Chromatography (SEC), also known as gel filtration chromatography, is a widely used method in HPLC & UHPLC for the separation of biomolecules based on their molecular size. Unlike other chromatographic techniques, SEC does not depend on chemical interactions between the analyte and the stationary phase. Instead, separation occurs through a porous stationary phase, where molecules are sorted according to their hydrodynamic volume. This approach ensures gentle and highly reproducible separations, making SEC ideal for applications such as protein and peptide analysis, oligonucleotide characterisation, and the detection of monoclonal antibody (mAb) aggregates.
How Do SEC Columns Work?
The separation process in SEC is based on molecular exclusion, meaning that smaller molecules enter the pores of the stationary phase and are retained longer, whereas larger molecules are excluded from the pores and elute faster. This mechanism allows for precise separation without unwanted secondary interactions. The method is widely used in biopharmaceutical research, protein purification, and quality control, where accurate molecular weight determination is critical.
Choosing the Right SEC Column for Your Application
YMC offers a selection of SEC columns tailored to different bioanalytical needs:
- YMC-Pack Diol SEC columns: Designed for a broad range of biomolecules, from small peptides to large proteins, with multiple pore sizes available (60, 120, 200, and 300 Å) to optimise separation efficiency.
- YMC-SEC MAB columns: Specifically developed for the analysis of monoclonal antibodies (mAbs), providing exceptional resolution across both high and low molecular weight ranges, making them particularly effective for aggregate and fragment detection.
- Multiple column dimensions: Supporting both analytical and preparative scale separations, ensuring maximum flexibility for various SEC chromatography workflows.
Applications of SEC Chromatography in HPLC & UHPLC
ISEC is an essential technique in many bioanalytical workflows, particularly in biopharmaceutical research, where it is used for protein characterisation, biosimilar development, and vaccine analysis. It is also widely applied in protein purification and quality control, ensuring batch-to-batch consistency in biotherapeutic formulations. Another key application is aggregation studies, where SEC enables the reliable detection and quantification of protein aggregates, ensuring the stability and efficacy of pharmaceutical products.
For expert advice on selecting the optimal SEC column for your analytical needs, contact YMC Europe today.