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Use our Application Search to discover chromatography solutions for HPLC and UHPLC. Filter by industry, compound, or column type to find the ideal match for your analysis.
Advantages
BioPro Ion Exchange Columns
Non-porous or porous particles
High loadability and recovery
Very high resolution
High dynamic binding capacity
Long term stability
Excellent lot-to-lot reproducibility
High throughput capability
Minimal non-specific adsorption
PRODUCT MEDIA
Reliable Control for
Complex Biomolecules
Downloads
Ion Exchange Columns Brochure
Download our detailed product brochure for in-depth insights into YMC Biochromatography columns or access the complete media package with extended materials.
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Applications
| Application | Column | Compound name | |
| DNA fragments | BioPro IEX QF | DNA fragments | |
| Lambda DNA Hind Ⅲ/EcoRⅠ restriction fragments | BioPro IEX QF | Lambda DNA Hind III/EcoR I digest (130-23, 130 bp) | |
| Monoclonal antibody (MAb) | BioPro IEX SF | Humanized monoclonal IgG1 | |
| Peptide mapping | BioPro IEX QA | Peptide mapping | |
| Plasmid pBR322 and pBR322 Hae III restriction fragments | BioPro IEX QF | Plasmid pBR322 Hae III digest (8-587 bp) Plasmid pBR322 (4361 bp) | |
| Proteins in human serum | BioPro IEX QA | IgG Transferrin Albumin | |
| Proteins | BioPro IEX QA | Ovalbumin Trypsin inhibitor (soybean) | |
| Synthetic oligonucleotides (Single-strand DNA) | BioPro IEX QF | 5’-TCATCACACTGAATACCAAT-3’ (20 mer) 5’-GTCATCACACTGAATACCAAT-3’ (21 mer) | |
| Synthetic oligonucleotides (Single-strand RNA) | BioPro IEX QF | 5’-U(M)C(M)A(M)U(M)C(M)A(M)C(M)A(M)C(M) U(M)G(M)A(M)A(M)U(M)A(M)C(M)C(M)A(M)A(M)U(M)-3’ (20 mer) 5’-G(M)U(M)C(M)A(M)U(M)C(M)A(M)C(M)A(M)C(M)U(M) G(M)A(M)A(M)U(M)A(M)C(M)C(M)A(M)A(M)U(M)-3’ (21 mer) |
Specifications
|
|
BioPro IEX QF
YMC Accura BioPro IEX QF
|
BioPro IEX SF
YMC Accura BioPro IEX SF
|
BioPro IEX QA
|
BioPro IEX SP
|
||
|
Base Material
|
hydrophilic polymer (polymethacrylate)
|
|||||
|
Particle Size [µm]
|
3, 5 µm
|
5 µm
|
||||
|
Pore Size [nm]
|
non-porous
|
100
|
||||
|
pH range
|
2.0 - 12.0
|
|||||
|
Column hardware
|
PEEK
bioinert coated stainless steel (YMC Accura)
|
PEEK
|
||||
|
Temperature range
|
4 - 60°C (PEEK)
4 - 80°C (YMC Accura)
|
4 - 60°C
|
||||
Phase Details
BioPro IEX QF/SF / YMC Accura BioPro IEX QF/SF
|
(formerly)
|
BioPro IEX QF
YMC Accura BioPro IEX QF
|
BioPro IEX SF
YMC Accura BioPro IEX SF
|
|
Matrix
|
Hydrophilic polymer (polymethacrylate)
|
|
|
Particle Size
|
3, 5 µm
|
|
|
Pore Size
|
Non-porous
|
|
|
Charged group
|
-CH2N+(CH3)3
(strong anion exchanger)
|
-(CH2)3SO3-
(strong cation exchanger)
|
|
Counter ion
|
Cl-
|
Na+
|
|
Ion-exchange capacity
|
0.075-0.110 meq/ml-resin
|
0.230-0.290 meq/ml-resin
|
|
Dynamic binding capacity
|
12-20 mg BSA/ml-resin
|
10-18 mg human IgG/ml-resin
|
|
pH range
|
2.0 - 12.0
|
|
|
Column hardware
|
PEEK
bioinert coated stainless steel (YMC Accura)
|
|
|
Temperature range
|
4 - 60°C (PEEK)
4 - 80°C (YMC Accura)
|
|
|
Pressure limit
|
3 µm: 25 MPa (3,625 psi, PEEK); 15 - 20 MPa (2,175 - 2,900 psi, YMC Accura)
5 µm: 6 - 12 MPa (870 - 1,740 psi, PEEK); 10 - 30 MPa (1,450 - 4,350 psi, YMC Accura)
|
|
The non-porous BioPro IEX particles offer high efficiency, exceptional resolution, and low operating pressures.
BioPro IEX QA/SP
|
(formerly)
|
BioPro IEX QA
|
BioPro IEX SP
|
||
|
Matrix
|
Hydrophilic polymer (polymethacrylate)
|
|||
|
Particle Size
|
5 µm
|
|||
|
Pore Size
|
100 nm / 1000 Å
|
|||
|
Charged group
|
-CH2N+(CH3)3
(strong anion exchanger)
|
-(CH2)3SO3-
(strong cation exchanger)
|
||
|
Counter ion
|
Cl-
|
Na+
|
||
|
Ion-exchange capacity
|
0.075-0.100 meq/ml-resin
|
0.070-0.095 meq/ml-resin
|
||
|
Dynamic binding capacity
|
110-150 mg BSA/ml-resin
|
70-100 mg human IgG/ml-resin
|
||
|
pH range
|
2.0 - 12.0
|
|||
|
Temperature range
|
4 - 60°C
|
|||
|
Column hardware
|
PEEK
|
|||
|
Pressure limit
|
2.5 - 3.5 MPa (360 - 510 psi)
|
|||
Technical Library
| Title | Document Type | Language | Download |
 (U)HPLC analysis of siRNA under denaturing and non-denaturing conditions using IP-RP and AEX |
Application Note | EN | |
| Analysis of mRNA coupled to enhanced green fluorescent protein by RP and IEX |
Application Note | EN | |
| Charge heterogeneity characterisation of an IgG4-based mAb using AEX coupled to MS |
Application Note | EN | |
| HPLC Analysis of Oligonucleotides with BioPro IEX QF |
Application Note | EN | |
| High resolution analysis of monoclonal antibodies using the cation exchange column BioPro IEX SF |
Application Note | EN | |
| MS compatible charge variant analysis of 28 commercial MAbs by CEX |
Application Note | EN | |
| Native AEX-MS analysis of IgG4-based mAbs |
Application Note | EN | |
| Native IEX-MS analysis of monoclonal antibodies using YMC Accura BioPro IEX SF columns |
Application Note | EN | |
| Native online SCX-MS analysis of monoclonal antibodies using YMC's BioPro IEX SF |
Application Note | EN | |
| Optimisation of oligonucleotide separations using YMC´s non porous anion exchanger BioPro IEX QF |
Application Note | EN | |
| Optimisation of phosphorothioate oligonucleotide separation using BioPro IEX QF |
Application Note | EN | |
| YMC Application Collection - Antibodies & ADCs |
Application Note | EN | |
 YMC Oligonucleotide Columns |
Brochure | EN | |
 BioPro IEX Columns Care and Use |
Care and Use Instructions | EN | |
 YMC Biochromatography Columns |
Catalogue | EN | |
| YMC Biochromatography Columns - IEX Columns Chapter |
Catalogue | EN | |
 Minimising carryover in oligonucleotide analysis by AEX |
Expert Tip | EN | |
 Analytical Stationary Phases for BioLC from YMC |
Overview | EN | |
| YMC Alternatives to Existing BioLC Columns |
Overview | EN | |
 A Stability-Indicating Method for Separation of Charge Variant Impurities of a PEGylated Protein by CEX-HPLC |
Poster | EN | |
| An Evaluation of YMC’s Strong Cation-Exchange Stationary Phase for the Analysis of Charged Isoforms of a Commercially Available Antibody Drug |
Poster | EN | |
| Oligonucleotide purification using anion exchange (AEX) and ion-pair reversed phase chromatography (IP-RP LC) |
Poster | EN | |
| Optimisation of oligonucleotide separations on ion exchange chromatography |
Poster | EN | |
| The Principles of BioLC & YMC BioLC Phase Overview |
Poster | EN | |
| Use of Small Particle YMC SEC and IEX Materials for Improved Characterization of Monoclonal Antibodies |
Poster | EN | |
 Advantages of strong cation exchangers vs. weak cation exchangers |
Technical Note | EN | |
| Characterisation of charge heterogeneity of therapeutic monoclonal antibodies via IEX-MS using BioPro IEX SF from YMC |
Technical Note | EN | |
| High temperature stability of BioPro IEX columns |
Technical Note | EN | |
| How to optimise your oligonucleotide analysis |
Technical Note | EN | |
| Utilisation of AEX-MS for charge variant analysis of IgG4-based mAbs! |
Technical Note | EN | |
 The Utility of a Cation Exchange Column for Successful Charge-Variant Analysis of PEGylated Proteins |
Whitepaper | EN |
Ordering Information
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| Product | Description | Price | Stock |
| QAA0S05-0346WP | BioPro IEX QA, PEEK HPLC column (4.6 mm ID), 100 nm, S-5 µm, 30 x 4.6 mm | - 2117 CHF 1727 € | |
| QAA0S05-0546WP | BioPro IEX QA, PEEK HPLC column (4.6 mm ID), 100 nm, S-5 µm, 50 x 4.6 mm | - 2117 CHF 1727 € | |
| QAA0S05-1046WP | BioPro IEX QA, PEEK HPLC column (4.6 mm ID), 100 nm, S-5 µm, 100 x 4.6 mm | - 2282 CHF 1861 € | |
| QF00S03-0346WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 30 x 4.6 mm | - 1510 CHF 1232 € | |
| QF00S03-03Q1PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-3 µm, 30 x 2.1 mm | - 2214 CHF 1806 € | |
| QF00S03-0546PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-3 µm, 50 x 4.6 mm | - 2214 CHF 1806 € | |
| QF00S03-0546WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 50 x 4.6 mm | - 1812 CHF 1478 € | |
| QF00S03-05Q1PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-3 µm, 50 x 2.1 mm | - 2214 CHF 1806 € | |
| QF00S03-1046PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-3 µm, 100 x 4.6 mm | - 2448 CHF 1997 € | |
| QF00S03-1046WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 100 x 4.6 mm | - 2040 CHF 1664 € | |
| QF00S03-10Q1PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-3 µm, 100 x 2.1 mm | - 2448 CHF 1997 € | |
| QF00S03-1546PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-3 µm, 150 x 4.6 mm | - 3032 CHF 2473 € | |
| QF00S03-1546WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 150 x 4.6 mm | - 2473 CHF 2017 € | |
| QF00S03-15Q1PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-3 µm, 150 x 2.1 mm | - 3032 CHF 2473 € | |
| QF00S03-E546GCC | YMC Accura BioPro IEX QF, non-porous, bioinert guard cartridges (4.6 mm ID), S-3 µm, 5 x 4.6 mm | - 1449 CHF 1182 € | |
| QF00S03-E5Q1GCC | YMC Accura BioPro IEX QF, non-porous, bioinert guard cartridges (2.1 mm ID), S-3 µm, 5 x 2.1 mm | - 1449 CHF 1182 € | |
| QF00S05-0346WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 30 x 4.6 mm | - 1761 CHF 1436 € | |
| QF00S05-03Q1PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-5 µm, 30 x 2.1 mm | - 2061 CHF 1681 € | |
| QF00S05-0546PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-5 µm, 50 x 4.6 mm | - 2061 CHF 1681 € | |
| QF00S05-0546WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 50 x 4.6 mm | - 2061 CHF 1681 € | |
| QF00S05-05Q1PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-5 µm, 50 x 2.1 mm | - 2061 CHF 1681 € | |
| QF00S05-1002WT | BioPro IEX QF, non-porous, narrowbore HPLC column (2.0 mm ID), S-5 µm, 100 x 2.0 mm | - 3061 CHF 2497 € | |
| QF00S05-1046PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-5 µm, 100 x 4.6 mm | - 2337 CHF 1906 € | |
| QF00S05-1046WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 100 x 4.6 mm | - 2337 CHF 1906 € | |
| QF00S05-10Q1PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-5 µm, 100 x 2.1 mm | - 2337 CHF 1906 € | |
| QF00S05-1546PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-5 µm, 150 x 4.6 mm | - 3015 CHF 2459 € | |
| QF00S05-1546WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 150 x 4.6 mm | - 3015 CHF 2459 € | |
| QF00S05-15Q1PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-5 µm, 150 x 2.1 mm | - 3015 CHF 2459 € | |
| QF00S05-2546PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-5 µm, 250 x 4.6 mm | - 3346 CHF 2729 € | |
| QF00S05-E546GCC | YMC Accura BioPro IEX QF, non-porous, bioinert guard cartridges (4.6 mm ID), S-5 µm, 5 x 4.6 mm | - 1449 CHF 1182 € | |
| QF00S05-E5Q1GCC | YMC Accura BioPro IEX QF, non-porous, bioinert guard cartridges (2.1 mm ID), S-5 µm, 5 x 2.1 mm | - 1449 CHF 1182 € | |
| QF00S06-1010WT | BioPro IEX QF, non-porous, semi-preparative HPLC column (10 mm ID), S-6 µm, 100 x 10 mm | - 15291 CHF 12472 € | |
| QF00S06-1020WT | BioPro IEX QF, non-porous, semi-preparative HPLC column (20 mm ID), S-6 µm, 100 x 20 mm | - 30624 CHF 24979 € | |
| QF00S06-1030WT | BioPro IEX QF, non-porous, semi-preparative HPLC column (30 mm ID), S-6 µm, 100 x 30 mm | - 61421 CHF 50099 € | |
| QF00S06-2546WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-6 µm, 250 x 4.6 mm | - 3505 CHF 2859 € | |
| SF00S03-0346WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 30 x 4.6 mm | - 1510 CHF 1232 € | |
| SF00S03-03Q1PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-3 µm, 30 x 2.1 mm | - 2214 CHF 1806 € | |
| SF00S03-0546PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-3 µm, 50 x 4.6 mm | - 2214 CHF 1806 € | |
| SF00S03-0546WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 50 x 4.6 mm | - 1812 CHF 1478 € | |
| SF00S03-05Q1PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-3 µm, 50 x 2.1 mm | - 2214 CHF 1806 € | |
| SF00S03-1046PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-3 µm, 100 x 4.6 mm | - 2448 CHF 1997 € | |
| SF00S03-1046WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 100 x 4.6 mm | - 2040 CHF 1664 € | |
| SF00S03-10Q1PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-3 µm, 100 x 2.1 mm | - 2448 CHF 1997 € | |
| SF00S03-1546PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-3 µm, 150 x 4.6 mm | - 3032 CHF 2473 € | |
| SF00S03-1546WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 150 x 4.6 mm | - 2473 CHF 2017 € | |
| SF00S03-15Q1PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-3 µm, 150 x 2.1 mm | - 3032 CHF 2473 € | |
| SF00S03-E546GCC | YMC Accura BioPro IEX SF, non-porous, bioinert guard cartridges (4.6 mm ID), S-3 µm, 5 x 4.6 mm | - 1449 CHF 1182 € | |
| SF00S03-E5Q1GCC | YMC Accura BioPro IEX SF, non-porous, bioinert guard cartridges (2.1 mm ID), S-3 µm, 5 x 2.1 mm | - 1449 CHF 1182 € | |
| SF00S05-0346WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 30 x 4.6 mm | - 1761 CHF 1436 € | |
| SF00S05-03Q1PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-5 µm, 30 x 2.1 mm | - 2061 CHF 1681 € | |
| SF00S05-0502WT | BioPro IEX SF, non-porous, narrowbore HPLC column (2.0 mm ID), S-5 µm, 50 x 2.0 mm | - 2679 CHF 2185 € | |
| SF00S05-0546PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-5 µm, 50 x 4.6 mm | - 2061 CHF 1681 € | |
| SF00S05-0546WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 50 x 4.6 mm | - 2061 CHF 1681 € | |
| SF00S05-05Q1PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-5 µm, 50 x 2.1 mm | - 2061 CHF 1681 € | |
| SF00S05-1002WT | BioPro IEX SF, non-porous, narrowbore HPLC column (2.0 mm ID), S-5 µm, 100 x 2.0 mm | - 3061 CHF 2497 € | |
| SF00S05-1008WT | BioPro IEX SF, non-porous, classical analytical HPLC column (8.0 mm ID), S-5 µm, 100 x 8.0 mm | - 6730 CHF 5489 € | |
| SF00S05-1046PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-5 µm, 100 x 4.6 mm | - 2337 CHF 1906 € | |
| SF00S05-1046WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 100 x 4.6 mm | - 2337 CHF 1906 € | |
| SF00S05-10Q1PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-5 µm, 100 x 2.1 mm | - 2337 CHF 1906 € | |
| SF00S05-1546PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-5 µm, 150 x 4.6 mm | - 3015 CHF 2459 € | |
| SF00S05-1546WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 150 x 4.6 mm | - 3015 CHF 2459 € | |
| SF00S05-15Q1PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-5 µm, 150 x 2.1 mm | - 3015 CHF 2459 € | |
| SF00S05-2546PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-5 µm, 250 x 4.6 mm | - 3346 CHF 2729 € | |
| SF00S05-E546GCC | YMC Accura BioPro IEX SF, non-porous, bioinert guard cartridges (4.6 mm ID), S-5 µm, 5 x 4.6 mm | - 1449 CHF 1182 € | |
| SF00S05-E5Q1GCC | YMC Accura BioPro IEX SF, non-porous, bioinert guard cartridges (2.1 mm ID), S-5 µm, 5 x 2.1 mm | - 1449 CHF 1182 € | |
| SF00S06-1010WT | BioPro IEX SF, non-porous, semi-preparative HPLC column (10 mm ID), S-6 µm, 100 x 10 mm | - 15291 CHF 12472 € | |
| SF00S06-1020WT | BioPro IEX SF, non-porous, semi-preparative HPLC column (20 mm ID), S-6 µm, 100 x 20 mm | - 30624 CHF 24979 € | |
| SF00S06-1030WT | BioPro IEX SF, non-porous, semi-preparative HPLC column (30 mm ID), S-6 µm, 100 x 30 mm | - 61421 CHF 50099 € | |
| SF00S06-2546WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-6 µm, 250 x 4.6 mm | - 3505 CHF 2859 € | |
| SPA0S05-0346WP | BioPro IEX SP, PEEK HPLC column (4.6 mm ID), 100 nm, S-5 µm, 30 x 4.6 mm | - 2117 CHF 1727 € | |
| SPA0S05-0546WP | BioPro IEX SP, PEEK HPLC column (4.6 mm ID), 100 nm, S-5 µm, 50 x 4.6 mm | - 2117 CHF 1727 € | |
| SPA0S05-1008WT | BioPro IEX SP, classical analytical HPLC column (8.0 mm ID), S-5 µm, 250 x 8.0 mm | - 7406 CHF 6041 € | |
| SPA0S05-1046WP | BioPro IEX SP, PEEK HPLC column (4.6 mm ID), 100 nm, S-5 µm, 100 x 4.6 mm | - 2282 CHF 1861 € | |
| XRPRCP02 | Precolumn filter 2 µm for 1/16" OD + holder, PEEK (350 bar) | - 370 CHF 302 € | |
| XRPRCP25 | Replacement precolumn filter 2 µm for 1/16" OD, PEEK (350 bar) | - 271 CHF 221 € |
Guide to Ion Exchange Chromatography (IEX) for HPLC & UHPLC
What Are Ion Exchange Columns?
Ion Exchange Chromatography (IEX) is a highly effective separation technique used in HPLC & UHPLC for biomolecule analysis and purification. It is based on the interaction between charged analytes and a charged stationary phase. IEX columns are designed to separate proteins, peptides, and nucleic acids based on their charge properties. There are two main types of Ion Exchange Columns:
- Cation Exchange Chromatography (CEX): Used for positively charged analytes, where a negatively charged stationary phase attracts cations.
- Anion Exchange Chromatography (AEX): Used for negatively charged analytes, where a positively charged stationary phase attracts anions.
YMC’s BioPro IEX columns are specifically developed for high-resolution separations, offering superior binding capacity and recovery compared to conventional IEX columns.
How Ion Exchange Chromatography Works
The separation process in ion exchange chromatography is influenced by several key factors:
- pH and buffer selection:The pH of the mobile phase affects the charge of both the stationary phase and the analyte, altering binding affinity
- Salt concentration: Gradual increases in salt concentration during elution help release bound analytes in a controlled manner.
- Porous vs. non-porous particles: Non-porous particles provide faster separations with higher resolution, while porous particles allow higher binding capacities for preparative applications.
YMC’s BioPro IEX columns utilize monodisperse, spherical polymer beads, ensuring optimal peak shapes and high reproducibility for anion and cation exchange chromatography.
Choosing the Right IEX Column for Your Application
Selecting the right IEX column depends on the size and charge properties of the biomolecule:
- BioPro IEX Q (strong anion exchanger) and BioPro IEX S (strong cation exchanger) are ideal for proteins and peptides.
- BioPro IEX Smart Columns provide high dynamic binding capacities, suitable for challenging biomolecule separations.
- Non-porous IEX columns enable high-speed separations in analytical workflows, while porous variants maximize loading capacity for preparative applications.
Applications of Ion Exchange Chromatography in HPLC & UHPLC
Ion Exchange Columns play a crucial role in biopharmaceutical research and analytical workflows, offering:
- High-resolution separations of proteins, peptides, and nucleotides
- Reliable performance in method development & validation
- Fast and efficient purification in biopharma and proteomics applications
Need help selecting the right IEX column for your bioseparation workflow? Contact YMC Europe for expert advice and customized solutions.