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Guide to Micro and Nano LC Columns for HPLC & UHPLC

What Are Micro Columns and Nano LC Columns?

Micro and Nano LC Columns, often referred to as capillary columns, are specialized chromatography columns with extremely small inner diameters, designed for high-sensitivity applications. These columns are ideal for working with minimal sample volumes and are frequently used in proteomics, metabolomics, and pharmaceutical research, especially in combination with mass spectrometry (LC/MS). YMC’s capillary columns for MicroLC and NanoLC are engineered for precise, reproducible separations with improved sensitivity and reduced sample and solvent consumption. Their compact format minimizes dead volume, making them perfectly suited for low-concentration sample analysis.

How Do Micro/Nano LC Columns Work?

Micro and nano LC columns operate at lower flow rates (typically in the nanoliter to low microliter range), reducing solvent consumption while increasing analyte concentration in the detector. These columns offer improved signal-to-noise ratios, making them ideal for trace-level analysis. The small particle sizes and tight column packing ensure sharp peaks and excellent resolution. They are compatible with most UHPLC systems and enable seamless integration into high-throughput analytical workflows.

Applications of Micro and Nano LC Columns

Micro and Nano LC Columns are particularly suited for:

  • Proteomics and biomarker discovery

  • Drug metabolism and pharmacokinetics (DMPK) studies

  • Trace analysis in clinical and environmental labs

  • LC/MS-based workflows with limited sample amounts

Advantages of YMC Capillary Columns

  • High sensitivity with reduced sample and solvent volumes

  • Excellent reproducibility and peak shapes

  • Full compatibility with LC/MS systems

  • Minimal band broadening due to reduced dead volume

  • Designed for robustness and long-term stability

Whether you're optimizing high-sensitivity bioanalytical methods or working with limited sample amounts, YMC’s Micro and Nano LC Columns offer a powerful solution for advanced analytical needs.

 

Need help choosing the right capillary column? Contact YMC Europe for expert consultation.

Contact us for consultation
  • YMC Europe GmbH
  • Phone +49 2064 427-0
  • Schöttmannshof 19
  • Fax +49 2064 427-222
  • D-46539 Dinslaken