Expert Topics

Troubleshooting Support for HPLC

Having issues with your HPLC system? Our troubleshooting guide offers solutions for common problems, ensuring optimal performance in your analytical processes.

Read more

Expertise portal: More expert content for you

In our expertise area, we have compiled extensive knowledge and supporting digital services for you. Use our extensive knowledge database and the numerous calculators of our expertise portal.

Read more

Online presentations: Experience us live

In our online presentations, we pass on our expert knowledge to you and answer all your questions. Book an online presentation on a specific topic and meet us live.

Read more

Guide to RP Bioseparation Columns for HPLC & UHPLC

What Are RP Bio Columns?

RP Bio Columns are specialized reversed-phase bioseparation columns designed for HPLC and UHPLC applications. These columns provide high-resolution separation of proteins, peptides, and biomolecules, ensuring optimal peak shapes and reproducibility. Their tailored pore sizes and hydrophobic surface properties make them ideal for biochromatography workflows.

How Do RP Bioseparation Columns Work?

RP bioseparation columns function by utilizing different stationary phases to separate biomolecules based on hydrophobicity, molecular weight, and surface interactions. Key factors influencing separation efficiency include:

  • Pore size selection: Crucial for peptides, proteins, and antibodies.

  • Hydrophobic interactions: Essential for biochromatography resolution.

  • Bioinert options: Prevent undesired interactions with nucleotides and oligonucleotides.

These properties make RP bio HPLC columns essential for analytical workflows in pharmaceutical analysis, biotechnology, and proteomics.

 

Applications of RP Bioseparation in HPLC & UHPLC

  • Separation of peptides, proteins & biomolecules

  • Enhanced peak symmetry with bioinert options

  • Optimized for RP biochromatography workflows

  • Reliable performance for method development & validation

 

For expert guidance on choosing the right RP bio column, contact YMC Europe today.

Contact us for consultation
  • YMC Europe GmbH
  • Phone +49 2064 427-0
  • Schöttmannshof 19
  • Fax +49 2064 427-222
  • D-46539 Dinslaken