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„My go-to column from now on“
„I’ve just finished running some injections on 2 YMC-Triart C18 columns [from 2 different lots] and I’m very impressed with them. The selectivity of YMC-Triart is better than all the other columns I’ve trialled, and the 2 batches have given me very similar chromatography. I’m very happy with them thank you for the recommendation, this may need to be my go-to column from now on!“
Becky Fok, Rosemont Pharmaceuticals (UK)
„Indispensable tools in our screening process.“
„YMC-Triart and CHIRAL ART columns, and their associated preparative purification solutions have become indispensable tools in our screening process for all new products, ensuring that we consistently achieve the highest level of achiral and chiral separation, from analytical to preparative scale.“
„Excellent resolution“
„We tried several different columns until we found YMC-Triart [Bio C18]. Great resolution, reproducibility and it is able to run a large number of analyses. We love it!!“
„Stable columns and good customer support“
„The possibility to use temperatures up to 90 °C with YMC-Triart Bio C4 simplifies the development of analytical methods. Furthermore, a good peak shape can be obtained without the addition of TFA, which means that I have fewer problems when using it for MS.“
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Troubleshooting Support for HPLC
Having issues with your HPLC system? Our troubleshooting guide offers solutions for common problems, ensuring optimal performance in your analytical processes.
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Guide to Analytical HPLC & UHPLC Columns
What is HPLC Chromatography?
High-Performance Liquid Chromatography (HPLC) is a widely used analytical technique for separating, identifying, and quantifying components in liquid mixtures. It operates by passing a liquid sample through a packed column containing a stationary phase, while a mobile phase (solvent) flows through the system. The components in the sample interact differently with the stationary phase, resulting in their separation. HPLC is critical in industries like pharmaceuticals, food safety, and environmental analysis because of its precision and high resolution.
The main components of an HPLC system include a pump for delivering the mobile phase, an autosampler for injecting the sample, a stationary phase column for separating the compounds, and a detector to measure the separated components. Other essential parts include tubing and connectors that ensure the smooth flow of the mobile phase through the system.
What is UHPLC Chromatography?
Ultra-High-Performance Liquid Chromatography (UHPLC) is an advanced version of HPLC, offering even faster and more precise separations. UHPLC operates at much higher pressures and uses smaller particle sizes (under 2 microns), resulting in greater resolution and speed. It’s especially useful for high-throughput environments, such as pharmaceutical development and proteomics, where efficiency and sharp peak separation are critical.
Differences Between HPLC and UHPLC
The main difference between HPLC and UHPLC lies in the operating pressures and particle sizes used in the columns. While HPLC typically uses particle sizes between 3 to 5 microns and operates at pressures up to 6,000 psi, UHPLC uses sub-2 micron particles and pressures of up to 15,000 psi. This allows UHPLC to achieve faster run times and higher resolution. HPLC remains more commonly used for routine analytical tasks, whereas UHPLC is ideal for more complex analyses that require higher speed and precision.
Types of HPLC & UHPLC Columns
Reversed Phase Columns: YMC’s RP columns are essential for separating non-polar compounds, supporting RP HPLC applications.
BioLC Columns: YMC’s BioLC columns are optimized for biomolecule separations like proteins and peptides, offering bioinert designs for sensitive biochromatography needs.
Bioinert Columns: Prevent interaction with sensitive biomolecules during HPLC.
RP Bio Columns: Used for RP bioseparation of proteins and peptides.
Ion-Exchange Columns: Separate charged molecules with IEX chromatography.
Size-Exclusion Columns (SEC): Ideal for separating molecules by size in SEC HPLC applications.
Hydrophobic Interaction Columns (HIC): Used for protein separation based on hydrophobicity.
Further HPLC & UHPLC columns
HILIC Columns: For polar compounds, HILIC columns are excellent for HILIC HPLC separations.
Normal Phase Columns: YMC offers normal phase HPLC columns for separating polar compounds using normal phase chromatography.
Chiral Columns: YMC’s chiral columns are designed for enantiomer separations in chiral chromatography.
Micro/NanoLC Columns: For high-sensitivity analyses, YMC provides Micro and Nano LC columns.
SFC Columns: YMC’s SFC columns are ideal for supercritical fluid chromatography (SFC) and chiral separations.
Semi-Preparative Columns (YMC Actus): For scaling up, YMC’s semi-preparative HPLC columns are perfect for handling larger sample volumes.
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