RP Columns
Downloads
YMC Pilot Columns Brochures
Download our detailed product brochures for in-depth insights or the complete media package on our Glass Columns to discover perfection in every detail.
Your next step
to first class products
Would you like to learn more about our products or
schedule a personal appointment? Please feel free to contact us!
Expert Topics
Guide to Reversed Phase Columns for HPLC and UHPLC
What is Reversed Phase Chromatography?
Reversed phase chromatography (RPC) is one of the most widely used techniques in liquid chromatography. In this method, the stationary phase is non-polar, typically consisting of hydrophobic ligands like C18 or C8 chains bonded to silica or polymer-based materials. The mobile phase, on the other hand, is polar, often consisting of water mixed with organic solvents like acetonitrile or methanol. This setup allows for the separation of compounds based on their hydrophobicity. Non-polar compounds bind more strongly to the stationary phase and are eluted later than polar compounds. This method is particularly suited for reversed phase HPLC and UHPLC applications, making it essential for separating a wide range of molecules, including organic compounds, peptides, and proteins.
Types of Reversed Phase Columns
YMC offers a broad selection of reversed phase columns, delivering high performance across a range of analytical applications:
YMC-Triart RP Columns: Known for their versatility, these columns offer excellent resolution and are ideal for various RP HPLC and UHPLC applications, providing consistent results in reverse phase separations.
YMC-Pro Family RP Columns: These columns ensure reliable and reproducible results, even in challenging reversed phase column chromatography processes. They are designed for routine as well as complex separations.
Meteoric Core RP Columns: Featuring core-shell technology, these columns deliver ultra-fast separations with high efficiency, making them a go-to solution for high-throughput HPLC and UHPLC workflows.
YMC Classics RP Columns: A proven choice for reliable performance, these columns are ideal for routine analyses, providing dependable results in reversed-phase chromatographie.
Why Choose Reversed Phase Columns?
YMC’s reversed phase HPLC columns are designed to ensure optimal separation of non-polar compounds across a variety of analytical HPLC and UHPLC applications. Whether you're working in pharmaceuticals, environmental analysis, or food safety, these columns offer high resolution, reproducibility, and robust performance. Reversed phase chromatography is preferred over normal phase chromatography due to its versatility and ability to handle a wide range of compounds, including polar, non-polar, and amphoteric substances.
Applications and Benefits
Reversed phase HPLC columns are ideal for method development, routine quality control, and complex separations. They offer the advantage of gradient elution, allowing precise control of separation by adjusting the polarity of the mobile phase. This results in higher resolution and faster analyses in RP phase applications.
For detailed information and personalized advice, YMC Europe is here to assist you. Contact us to find the optimal solution for your needs.