Oligonucleotides are highly important biopharmaceuticals with strongly increasing relevance. Due to the high similarity of impurities originating from the synthesis, however, purification can be challenging.

This seminar demonstrates a successful large scale purification of an oligonucleotide using anion exchange chromatography (AEX) as well as efficient quality control by applying ion-pairing reversed phase chromatography (IP-RP).

Peptides have become one of the most important biopharmaceuticals. The high variability related to chain length and sequence of amino acids offers multiple pharmaceutical applications, and leads to specific demands regarding the production and purification.

This seminar therefore aims to provide the knowledge required for development of peptide purification methods based on case studies of up-to-date targets like a GLP-1 agonist and a peptide hormone.