Sharp peaks and reliable recovery
Oligonucleotides (here: 21mer RNA) generally exhibit poor peak shapes and therefore low recovery in AEX analysis, mainly due to adsorption onto the column hardware. YMC Accura BioPro IEX QF columns provide high recovery and very good peak shapes making them ideal for these analyses with reproducible results. The columns show stable peak areas from the 1st injection, so that no preconditioning is required.
No preconditioning required for reliable results
The high inertness of YMC Accura BioPro IEX columns requires no preconditioning in CEX analyses (here: bevacizumab). Especially at low loading amounts, YMC Accura BioPro IEX SF columns provide higher recoveries compared to the standard PEEK column.
Ideally suited for native IEX-MS
Smaller column inner diameters that allow lower flow rates and volatile mobile phases are necessities for coupling to mass detection. Such high sensitivity analyses are ideally performed using YMC Accura BioPro IEX columns (here: trastuzumab). Another positive effect is the decreased solvent consumption and lower amount of sample required.