More and more IgG4-based monoclonal antibodies (mAbs) with lower pIs (< 8) are now being developed. Therefore, anion exchange chromatography (AEX) is a promising alternative approach to the commonly used cation exchange chromatography (CEX) for IgG1-based mAbs.

This technical note demonstrates the successful application of AEX-MS for the charge variant analysis of different IgG4-based mAbs using a BioPro IEX QF column. The AEX method shows overall better separation and provides additional information.

The resolution can be further improved by lowering the pI with PNGase F-mediated deglycosylation. In addition, critical Fc quality attributes can be monitored. This means that with this method, complex and time-consuming peptide mapping is no longer necessary.

Facilitate your mAb characterisation! Download the extensive technical note!

Find out more about YMC’s premium solutions for mAbs and download the YMC Biochromatography Columns catalogue.

On October 18^th and 19^th LCGC’s virtual symposium Separation Science - The State of The Art in Life Science Analysis took place.

Did you miss it? No worries!

All talks are available on demand. In addition to interesting talks from several scientists watch YMC’s talk Improving chromatographic results by the use of bioinert column hardware and learn more about:

• Which biomolecules tend to interact with metal surfaces.
• How bioinert column hardware can improve your analysis.

What the differences are between the hardware options and how to select the most suitable.