Lars M. H. Reinders from the Institute for Energy and Environmental Technology e.V. (IUTA) presents in this online presentation the mass spectrometric analysis of monoclonal antibodies. He discusses one-dimensional as well as two-dimensional approaches. The presentation starts with one-dimensional antibody analysis. The special features of intact proteins and peptides are the focus here. The presentation continues with multidimensional antibody analytics. The application possibilities of two-dimensional antibody analytics are clearly presented and the following questions are clarified: Which modes are there? When is which mode used? Subsequently, alternative uses of 2D-HPLC-MS are considered, i.e. the diverse automation possibilities.