Verfügbare Stationärphasen:
Die BioPro IEX Säulen zeichnen sich aus durch:
- Unporöse oder poröse Partikel
- Sehr gute Auflösung
- Herausragende Stabilität
- Hohe Beladbarkeit und Wiederfindung
- Hohe dynamische Bindungskapazität
- Exzellente Lot-zu-Lot-Reproduzierbarkeit
Applikationen
Anwendung | Säule | Verbindung | |
DNA fragments | BioPro IEX QF | DNA fragments | |
Lambda DNA Hind Ⅲ/EcoRⅠ restriction fragments | BioPro IEX QF | Lambda DNA Hind III/EcoR I digest (130-23, 130 bp) | |
Monoclonal antibody (MAb) | BioPro IEX SF | Humanized monoclonal IgG1 | |
Peptide mapping | BioPro IEX QA | Peptide mapping | |
Plasmid pBR322 and pBR322 Hae III restriction fragments | BioPro IEX QF | Plasmid pBR322 Hae III digest (8-587 bp) Plasmid pBR322 (4361 bp) | |
Proteins in human serum | BioPro IEX QA | IgG Transferrin Albumin | |
Proteins | BioPro IEX QA | Ovalbumin Trypsin inhibitor (soybean) | |
Synthetic oligonucleotides (Single-strand DNA) | BioPro IEX QF | 5’-TCATCACACTGAATACCAAT-3’ (20 mer) 5’-GTCATCACACTGAATACCAAT-3’ (21 mer) | |
Synthetic oligonucleotides (Single-strand RNA) | BioPro IEX QF | 5’-U(M)C(M)A(M)U(M)C(M)A(M)C(M)A(M)C(M) U(M)G(M)A(M)A(M)U(M)A(M)C(M)C(M)A(M)A(M)U(M)-3’ (20 mer) 5’-G(M)U(M)C(M)A(M)U(M)C(M)A(M)C(M)A(M)C(M)U(M) G(M)A(M)A(M)U(M)A(M)C(M)C(M)A(M)A(M)U(M)-3’ (21 mer) |
Spezifikationen
|
BioPro IEX QF
YMC Accura BioPro IEX QF
|
BioPro IEX SF
YMC Accura BioPro IEX SF
|
BioPro IEX QA
|
BioPro IEX SP
|
||
Basismaterial
|
hydrophiles Polymer (Polymethacrylat)
|
|||||
Partikelgröße [µm]
|
3, 5 µm
|
5 µm
|
||||
Porengröße [nm]
|
unporös
|
100
|
||||
pH-Bereich
|
2.0 - 12.0
|
|||||
Säulenhardware
|
PEEK
bioinert gecoateter Edelstahl (YMC Accura)
|
PEEK
|
||||
Temperaturbereich
|
4 - 60°C (PEEK)
4 - 80°C (YMC Accura)
|
4 - 60°C
|
Phasendetails
BioPro IEX QF/SF / YMC Accura BioPro IEX QF/SF
|
BioPro IEX QF
YMC Accura BioPro IEX QF
|
BioPro IEX SF
YMC Accura BioPro IEX SF
|
Basismaterial
|
Hydrophiles Polymer (Polymethacrylat)
|
|
Partikelgröße
|
3, 5 µm
|
|
Porengröße
|
unporös
|
|
Geladene Gruppe
|
-CH2N+(CH3)3
(starker Anionenaustauscher)
|
-(CH2)3SO3-
(starker Kationenaustauscher)
|
Gegenion
|
Cl-
|
Na+
|
Ionenaustausch-Kapazität
|
0.075-0.110 meq/ml-Resin
|
0.230-0.290 meq/ml-Resin
|
Dynamische Bindungskapazität
|
12-20 mg BSA/ml-Resin
|
10-18 mg humanes IgG/ml-Resin
|
pH-Bereich
|
2.0 - 12.0
|
|
Säulenhardware
|
PEEK
bioinert gecoateter Edelstahl (YMC Accura)
|
|
Temperaturbereich
|
4 - 60°C (PEEK)
4 - 80°C (YMC Accura)
|
|
Drucklimit
|
3 µm: 25 MPa (3,625 psi, PEEK); 15 - 20 MPa (2,175 - 2,900 psi, YMC Accura)
5 µm: 6 - 12 MPa (870 - 1,740 psi, PEEK); 10 - 30 MPa (1,450 - 4,350 psi, YMC Accura)
|
Die unporösen BioPro IEX Partikel zeigen eine hervorragende Effizienz, exzellente Auflösungen und niedrige Rückdrücke.
BioPro IEX QA/SP
BioPro IEX QA
|
BioPro IEX SP
|
|||
Basismaterial
|
Hydrophiles Polymer (Polymethacrylat)
|
|||
Partikelgröße
|
5 µm
|
|||
Porengröße
|
100 nm / 1000 Å
|
|||
Geladene Gruppe
|
-CH2N+(CH3)3
(starker Anionenaustauscher)
|
-(CH2)3SO3-
(schwacher Kationentauscher)
|
||
Gegenion
|
Cl-
|
Na+
|
||
Ionenaustausch-Kapazität
|
0.075-0.100 meq/ml-Resin
|
0.070-0.095 meq/ml-Resin
|
||
Dynamische Bindungskapazität
|
110-150 mg BSA/ml-Resin
|
70-100 mg humanes IgG/ml-Resin
|
||
pH-Bereich
|
2.0 - 12.0
|
|||
Temperaturbereich
|
4 - 60°C
|
|||
Säulenhardware
|
PEEK
|
|||
Drucklimit
|
2.5 - 3.5 MPa (360 - 510 psi)
|
Technische Dokumente
Titel | Dokumententyp | Sprache | Download |
(U)HPLC analysis of siRNA under denaturing and non-denaturing conditions using IP-RP and AEX | Application Note | EN | |
Analysis of mRNA coupled to enhanced green fluorescent protein by RP and IEX | Application Note | EN | |
Charge heterogeneity characterisation of an IgG4-based mAb using AEX coupled to MS | Application Note | EN | |
HPLC Analysis of Oligonucleotides with BioPro IEX QF | Application Note | EN | |
High resolution analysis of monoclonal antibodies using the cation exchange column BioPro IEX SF | Application Note | EN | |
MS compatible charge variant analysis of 28 commercial MAbs by CEX | Application Note | EN | |
Native AEX-MS analysis of IgG4-based mAbs | Application Note | EN | |
Native online SCX-MS analysis of monoclonal antibodies using YMC's BioPro IEX SF | Application Note | EN | |
Optimisation of oligonucleotide separations using YMC´s non porous anion exchanger BioPro IEX QF | Application Note | EN | |
Optimisation of phosphorothioate oligonucleotide separation using BioPro IEX QF | Application Note | EN | |
YMC Application Collection - Antibodies & ADCs | Application Note | EN | |
YMC Oligonucleotide Columns | Brochure | EN | |
BioPro IEX Columns Care and Use | Care and Use Instructions | EN | |
YMC Biochromatography Columns | Catalogue | EN | |
YMC Biochromatography Columns (HQ) | Catalogue | EN | |
YMC Biochromatography Columns - IEX Columns Chapter | Catalogue | EN | |
Minimising carryover in oligonucleotide analysis by AEX | Expert Tip | EN | |
Analytical Stationary Phases for BioLC from YMC | Overview | EN | |
YMC Alternatives to Existing BioLC Columns | Overview | EN | |
A Stability-Indicating Method for Separation of Charge Variant Impurities of a PEGylated Protein by CEX-HPLC | Poster | EN | |
An Evaluation of YMC’s Strong Cation-Exchange Stationary Phase for the Analysis of Charged Isoforms of a Commercially Available Antibody Drug | Poster | EN | |
Oligonucleotide purification using anion exchange (AEX) and ion-pair reversed phase chromatography (IP-RP LC) | Poster | EN | |
Optimisation of oligonucleotide separations on ion exchange chromatography | Poster | EN | |
The Principles of BioLC & YMC BioLC Phase Overview | Poster | EN | |
Use of Small Particle YMC SEC and IEX Materials for Improved Characterization of Monoclonal Antibodies | Poster | EN | |
Advantages of strong cation exchangers vs. weak cation exchangers | Technical Note | EN | |
Characterisation of charge heterogeneity of therapeutic monoclonal antibodies via IEX-MS using BioPro IEX SF from YMC | Technical Note | EN | |
High temperature stability of BioPro IEX columns | Technical Note | EN | |
How to optimise your oligonucleotide analysis | Technical Note | EN | |
Utilisation of AEX-MS for charge variant analysis of IgG4-based mAbs! | Technical Note | EN | |
Vorteile von starken Kationaustauschern gegenüber schwachen Kationaustauschern | Technical Note | DE | |
Grundlagen der Chromatographie für die Analyse und Aufreinigung von Biologika | Whitepaper | DE | |
The Utility of a Cation Exchange Column for Successful Charge-Variant Analysis of PEGylated Proteins | Whitepaper | EN |
Bestellinformation
Bitte melden Sie sich an, um die Preise zu sehen.
- 2-3 Tage
- 1-2 Wochen
- 3-4 Wochen
Produkt | Beschreibung | Preis | Lagerbestand |
QAA0S05-0346WP | BioPro IEX QA, PEEK HPLC column (4.6 mm ID), 100 nm, S-5 µm, 30 x 4.6 mm | - 2054 CHF 1675 € | |
QAA0S05-0546WP | BioPro IEX QA, PEEK HPLC column (4.6 mm ID), 100 nm, S-5 µm, 50 x 4.6 mm | - 2054 CHF 1675 € | |
QAA0S05-1046WP | BioPro IEX QA, PEEK HPLC column (4.6 mm ID), 100 nm, S-5 µm, 100 x 4.6 mm | - 2213 CHF 1805 € | |
QF00S03-0346WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 30 x 4.6 mm | - 1465 CHF 1195 € | |
QF00S03-03Q1PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-3 µm, 30 x 2.1 mm | - 2148 CHF 1752 € | |
QF00S03-0546PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-3 µm, 50 x 4.6 mm | - 2148 CHF 1752 € | |
QF00S03-0546WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 50 x 4.6 mm | - 1758 CHF 1434 € | |
QF00S03-05Q1PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-3 µm, 50 x 2.1 mm | - 2148 CHF 1752 € | |
QF00S03-1046PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-3 µm, 100 x 4.6 mm | - 2375 CHF 1937 € | |
QF00S03-1046WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 100 x 4.6 mm | - 1979 CHF 1614 € | |
QF00S03-10Q1PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-3 µm, 100 x 2.1 mm | - 2375 CHF 1937 € | |
QF00S03-1546PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-3 µm, 150 x 4.6 mm | - 2941 CHF 2399 € | |
QF00S03-1546WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 150 x 4.6 mm | - 2398 CHF 1956 € | |
QF00S03-15Q1PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-3 µm, 150 x 2.1 mm | - 2941 CHF 2399 € | |
QF00S05-0346WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 30 x 4.6 mm | - 1708 CHF 1393 € | |
QF00S05-03Q1PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-5 µm, 30 x 2.1 mm | - 1998 CHF 1630 € | |
QF00S05-0546PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-5 µm, 50 x 4.6 mm | - 1998 CHF 1630 € | |
QF00S05-0546WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 50 x 4.6 mm | - 1998 CHF 1630 € | |
QF00S05-05Q1PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-5 µm, 50 x 2.1 mm | - 1998 CHF 1630 € | |
QF00S05-1002WT | BioPro IEX QF, non-porous, narrowbore HPLC column (2.0 mm ID), S-5 µm, 100 x 2.0 mm | - 2969 CHF 2422 € | |
QF00S05-1046PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-5 µm, 100 x 4.6 mm | - 2267 CHF 1849 € | |
QF00S05-1046WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 100 x 4.6 mm | - 2267 CHF 1849 € | |
QF00S05-10Q1PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-5 µm, 100 x 2.1 mm | - 2267 CHF 1849 € | |
QF00S05-1546PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-5 µm, 150 x 4.6 mm | - 2924 CHF 2385 € | |
QF00S05-1546WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 150 x 4.6 mm | - 2924 CHF 2385 € | |
QF00S05-15Q1PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-5 µm, 150 x 2.1 mm | - 2924 CHF 2385 € | |
QF00S05-2546PTC | YMC Accura BioPro IEX QF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-5 µm, 250 x 4.6 mm | - 3245 CHF 2647 € | |
QF00S06-1010WT | BioPro IEX QF, non-porous, semi-preparative HPLC column (10 mm ID), S-6 µm, 100 x 10 mm | - 14831 CHF 12097 € | |
QF00S06-1020WT | BioPro IEX QF, non-porous, semi-preparative HPLC column (20 mm ID), S-6 µm, 100 x 20 mm | - 29704 CHF 24228 € | |
QF00S06-1030WT | BioPro IEX QF, non-porous, semi-preparative HPLC column (30 mm ID), S-6 µm, 100 x 30 mm | - 59575 CHF 48593 € | |
QF00S06-2546WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-6 µm, 250 x 4.6 mm | - 3400 CHF 2773 € | |
SF00S03-0346WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 30 x 4.6 mm | - 1465 CHF 1195 € | |
SF00S03-03Q1PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-3 µm, 30 x 2.1 mm | - 2148 CHF 1752 € | |
SF00S03-0546PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-3 µm, 50 x 4.6 mm | - 2148 CHF 1752 € | |
SF00S03-0546WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 50 x 4.6 mm | - 1758 CHF 1434 € | |
SF00S03-05Q1PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-3 µm, 50 x 2.1 mm | - 2148 CHF 1752 € | |
SF00S03-1046PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-3 µm, 100 x 4.6 mm | - 2375 CHF 1937 € | |
SF00S03-1046WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 100 x 4.6 mm | - 1979 CHF 1614 € | |
SF00S03-10Q1PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-3 µm, 100 x 2.1 mm | - 2375 CHF 1937 € | |
SF00S03-1546PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-3 µm, 150 x 4.6 mm | - 2941 CHF 2399 € | |
SF00S03-1546WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 150 x 4.6 mm | - 2398 CHF 1956 € | |
SF00S03-15Q1PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-3 µm, 150 x 2.1 mm | - 2941 CHF 2399 € | |
SF00S05-0346WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 30 x 4.6 mm | - 1708 CHF 1393 € | |
SF00S05-03Q1PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-5 µm, 30 x 2.1 mm | - 1998 CHF 1630 € | |
SF00S05-0502WT | BioPro IEX SF, non-porous, narrowbore HPLC column (2.0 mm ID), S-5 µm, 50 x 2.0 mm | - 2598 CHF 2119 € | |
SF00S05-0546PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-5 µm, 50 x 4.6 mm | - 1998 CHF 1630 € | |
SF00S05-0546WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 50 x 4.6 mm | - 1998 CHF 1630 € | |
SF00S05-05Q1PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-5 µm, 50 x 2.1 mm | - 1998 CHF 1630 € | |
SF00S05-1002WT | BioPro IEX SF, non-porous, narrowbore HPLC column (2.0 mm ID), S-5 µm, 100 x 2.0 mm | - 2969 CHF 2422 € | |
SF00S05-1008WT | BioPro IEX SF, non-porous, classical analytical HPLC column (8.0 mm ID), S-5 µm, 100 x 8.0 mm | - 6527 CHF 5324 € | |
SF00S05-1046PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-5 µm, 100 x 4.6 mm | - 2267 CHF 1849 € | |
SF00S05-1046WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 100 x 4.6 mm | - 2267 CHF 1849 € | |
SF00S05-10Q1PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-5 µm, 100 x 2.1 mm | - 2267 CHF 1849 € | |
SF00S05-1546PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-5 µm, 150 x 4.6 mm | - 2924 CHF 2385 € | |
SF00S05-1546WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 150 x 4.6 mm | - 2924 CHF 2385 € | |
SF00S05-15Q1PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (2.1 mm ID), S-5 µm, 150 x 2.1 mm | - 2924 CHF 2385 € | |
SF00S05-2546PTC | YMC Accura BioPro IEX SF, non-porous, bioinert coated HPLC column (4.6 mm ID), S-5 µm, 250 x 4.6 mm | - 3245 CHF 2647 € | |
SF00S06-1010WT | BioPro IEX SF, non-porous, semi-preparative HPLC column (10 mm ID), S-6 µm, 100 x 10 mm | - 14831 CHF 12097 € | |
SF00S06-1020WT | BioPro IEX SF, non-porous, semi-preparative HPLC column (20 mm ID), S-6 µm, 100 x 20 mm | - 29704 CHF 24228 € | |
SF00S06-1030WT | BioPro IEX SF, non-porous, semi-preparative HPLC column (30 mm ID), S-6 µm, 100 x 30 mm | - 59575 CHF 48593 € | |
SF00S06-2546WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-6 µm, 250 x 4.6 mm | - 3400 CHF 2773 € | |
SPA0S05-0346WP | BioPro IEX SP, PEEK HPLC column (4.6 mm ID), 100 nm, S-5 µm, 30 x 4.6 mm | - 2054 CHF 1675 € | |
SPA0S05-0546WP | BioPro IEX SP, PEEK HPLC column (4.6 mm ID), 100 nm, S-5 µm, 50 x 4.6 mm | - 2054 CHF 1675 € | |
SPA0S05-1008WT | BioPro IEX SP, classical analytical HPLC column (8.0 mm ID), S-5 µm, 250 x 8.0 mm | - 7183 CHF 5859 € | |
SPA0S05-1046WP | BioPro IEX SP, PEEK HPLC column (4.6 mm ID), 100 nm, S-5 µm, 100 x 4.6 mm | - 2213 CHF 1805 € | |
XRPRCP02 | Precolumn filter 2 µm for 1/16" OD + holder, PEEK (350 bar) | - 353 CHF 288 € | |
XRPRCP25 | Replacement precolumn filter 2 µm for 1/16" OD, PEEK (350 bar) | - 257 CHF 210 € |