Analysis of benzodiazepines and their metabolites by UHPLC-MS/MS
The analysis of benzodiazepines in biological fluids is important to clinicians and forensic toxicologists because of their sedative, hypnotic, antianxiety, antiepileptic and muscle relaxant properties. However, it is challenging to analyse these compounds because their chemical structures, molecular weights and fragmentation patterns during mass spectrometry are very similar.
This application note shows a high-resolution UHPLC separation using a robust YMC-Triart C18 column including the previous sample preparation . The simultaneous analysis of etizolam, triazolam, and their metabolites 8-ethylhydroxyetizolam, alpha-hydroxyetizolam, 4-hydroxytriazolam and alpha-hydroxytriazolam in biological samples is performed by MS/MS detection.
Download the application note with the full method details.
In order to receive more information on YMC-Triart C18 and further YMC-Triart columns, simply download the YMC-Triart Brochure.
Cost-efficient Purification of Antibodies
How to obtain an efficient and reliable purification of antibodies?
The commercial success of antibody pharmaceuticals, depends on a careful consideration of the production costs. Process efficiency is strongly influenced by the binding capacity of the used resin, as this determines the amount of product that can be purified per chromatographic cycle.
A comparison study was conducted and BioPro IEX S30 was tested alongside competitive resins for the purification of a monoclonal, pharmaceutical antibody under various method conditions. This study is available for download here and confirms a significant gain in dynamic binding capacity due to the use of the YMC IEX resin.
Be sure to contact us for expert support regarding method development and scale up of your biochromatographic projects. What can we do for you?
For more information we invite you to see our website here.
New to YMC’s portfolio: YMC vials for HPLC
The choice of a suitable vial is not only dependent on the autosampler compatibility, but also on the intended purpose.
Requirements such as inertness, filling volume and material of the vial are critical. The new YMC Vial portfolio will meet the needs of your sample.
Find out more about YMC vial portfolio here.
We will be pleased to advise you personally, if you require further information.
Make Use of Expert Tips: Sign up now for a YMC seminar!
We invite you to take part in the following seminars:
- Glass Column Packing – Laboratory Scale and Pilot Columns
November 7th, 2019
Target Group: Glass column users with alternative qualifications (laboratory assistants, technicians, engineers, chemists)
Fee: 1,700 € p.p. plus VAT
- Preparative chromatography – Process development and scale-up
November 12th, 2019
Target Group: All chromatography operators who deal with developing and operating preparative separations
Fee: 985 € p.p. plus VAT
Click here to sign up.