Application Note:
Oxidised monoclonal antibodies using HIC

 

Do you need to ensure efficacy and safety of biopharmaceutical products such as monoclonal antibodies (MAbs)?
Is characterisation of antibody variants, due to product safety, of interest for you?
 
Learn with this application note how oxidised variants of MAbs can be analysed using hydrophobic interaction chromatography (HIC). The application benefits on YMC’s BioPro HIC BF, which is specifically designed for proteins of low hydrophobicity and oxidised MAb variants.
 
Download the application note with the full method details here.
Please download YMC’s BioLC catalogue or visit our homepage to receive more information on YMC’s BioPro HIC columns.

 

Application Note: Oxidised monoclonal antibodies using HIC 

 

New Application Note:
Online native HIC-MS analysis of MAbs

 

YMC MicroLC/NanoLC columns, especially packed with YMC-Triart stationary phases, provide you superior efficiencies for your analyses!

Do you characterise MAbs using HIC?
Do you want to couple HIC directly to MS without the need for peak isolation beforehand?

In this application note a mixture of seven MAbs is characterised via online native HIC-MS. It shows nicely that innovative columns such as YMC’s BioPro HIC BF column are broadening the chromatographer’s tool-box. It demonstrates how to overcome the high salt concentrations that are required for HIC separations.

Download the application note now.


 

Fig.: Separation of an antibody mixture of seven different MAbs.

Fig.: Separation of an antibody mixture of seven different MAbs.

 

Robust (U)HPLC columns for peptides and oligonucleotides

 

Are you looking for robust and reproducible columns to evaluate peptides?
Do you want to separate oligonucleotides with high resolution?
 
Robust YMC-Triart C18 columns are the perfect choice for reliable and reproducible (U)HPLC analyses of peptides and oligonucleotides with exceptional resolution. The wide temperature- (up to 90°C) and pH-range (1-12) provide increased flexibility. Full MS compatibility is guaranteed due to virtually no column bleeding. Optionally available metal-free bioinert hardware inhibits unwanted interactions and ensures highly sensitive analyses.
 
Features of YMC-Triart C18 columns are:

  • Superior lot-to-lot reproducibility
  • High sensitivity due to full MS compatibility and optional bioinert hardware
  • Extremely robust and long lasting

Check out YMC’s BioLC catalogue or visit our homepage to discover more about YMC-Triart C18 or find helpful applications.

 

Robust (U)HPLC columns for peptides and oligonucleotides

 

 

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