siRNAs are typically analysed by ion pair reversed phase chromatography (IP-RP) or anion exchange chromatography (AEX). Different chromatographic conditions allow the analysis of double-stranded duplexes (non-denaturing) or single strands (denaturing conditions).
This technical note describes the method development for non-denatured and denatured siRNA using a non-porous BioPro IEX QF column.
Various method parameters are considered:
- Influence of pH, salts and temperature
- Organic solvents
- Additives to the mobile phase
In addition, various method parameters for purification are also described.
Please refer to the Technical Note for full details.
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