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Maximising the separation capacity by coupling two SEC columns

The use of only one SEC column brings a certain limitation due to its dedicated pore size and a limited column volume. By coupling two columns this hurdle can be overcome. Doubling the column length implies a doubled column volume which results in improved resolution of peaks. Additionally, if using two columns with different pore size, the applicable molecular weight range can be increased significantly. In this application note, the analysis of plasma constituents using two coupled YMC-Pack Diol columns with different pore sizes is shown.

 

By using this setup, the applicable molecular weight range can be extended by up to 700 kDa.

Please download the application note for further details.

In order to receive more information on YMC SEC columns visit our homepage or download the YMC BioLC catalogue.

 

Learn more about the resolving power of non-porous HIC columns!

This technical note answers questions regarding how intact protein analyses can be improved by using non-porous HIC columns.
Various influencing parameters are addressed that need to be considered when using non-porous HIC columns:

  • Salt concentration
  • Temperature
  • Flow rate and gradient time
  • Column length and particle diameter

Download your copy of the report here and learn how optimising these parameters can improve your intact protein analysis.

In order to receive more information on YMC HIC columns please visit our homepage or download the YMC BioLC catalogue.

 

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