The use of only one SEC column brings a certain limitation due to its dedicated pore size and a limited column volume. By coupling two columns this hurdle can be overcome. Doubling the column length implies a doubled column volume which results in improved resolution of peaks. Additionally, if using two columns with different pore size, the applicable molecular weight range can be increased significantly. In this application note, the analysis of plasma constituents using two coupled YMC-Pack Diol columns with different pore sizes is shown.
By using this setup, the applicable molecular weight range can be extended by up to 700 kDa.
Please download the application note for further details.
In order to receive more information on YMC SEC columns visit our homepage or download the YMC BioLC catalogue.