EXPERT TIP: Purification of peptides – Increasing the purity and recovery by salt addition
Extended options for process optimisation
The purification of peptides can be very challenging. Depending on the complexity of the sample feed, the use of regularly used methods may not be sufficient.
This expert tip demonstrates the great potential of adding a salt to the mobile phase for the purification of insulin in order to achieve the required purity and recovery. Adding salt to the mobile phase can greatly improve the separation for the isolation of peptide- and oligonucleotide-based compounds. It extends the range of possible options available for process optimisation.
Application Note: Enhanced peptide purification
This application note shows life data obtained from insulin manufacturing. These examples demonstrate the great potential for the process optimisation and the development of new processes for the purification of peptides and oligonucleotides using highly efficient stationary phases and optimal conditions.
