When developing a new method, the selection of eluents is always a key factor. pH has an especially important influence on the retention when working with ionisable compounds. At a pH ± 2 units around the analyte’s pKa, even small changes in pH can lead to drastic retention shifts.
This guideline includes the following helpful tips and overviews:
- How to select the optimum pH
- Selecting the corresponding optimum buffer
- A list of common buffers, their buffer range and standard concentration and their MS compatibility
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