Phosphoramidites are the essential part in the chemical synthesis of oligonucleotides, short fragments of nucleotides and analogues. During the synthesis, errors can occur which is why the phosphoramidites’ purity needs to be closely monitored.

In this application note, it is shown how to analyse phosphoramidites using two different separation modes: reversed phase (RP) and normal phase (NP) chromatography. Due to the complexity of the analytes different interactions can be used in order to achieve acceptable retenetion and resolution.

Download your copy here and see how YMC-Triart columns show excellent results using either RP or NP separation modes.

 

With HILIC separations the elution order is completely reversed to that in RP mode, making it an ideal choice for the analysis of hydrophilic compounds. As these compounds elute late on HILIC columns, this separation mode is well suited for the analysis of hydrophilic biomolecules.

Four different, hydrophilic dipeptides could be separated using a YMC-Triart SIL column, as shown in this new application note. L-Carnosine and L-anserine can be found in muscle and brain tissues in mammals. Gly-Gly is used in peptide synthesis whereas Gly-Asp is commonly known as a metabolite.

The YMC-Triart SIL column shows excellent resolution for all dipeptides, even for the structurally similar compounds L-carnosine and L-anserine. Download your copy of the application note here and see how this HILIC separation can be performed in under four minutes!