YMC i-Mail March 2023

New Application Note

How to determine the purity of a crude oligonucleotide

A practical example for qualification and quantification of a complex oligonucleotide sample by IP-RP UHPLC prior to further purification has been produced. This very comprehensive approach had been published by Fioretti et al.

This Application Note shows the separation of a crude oligonucleotide sample by using a
YMC-Triart C18 UHPLC in IP-RP mode.

To obtain a full copy of this Application Note, see below.

More information on YMC-Triart columns can be found in the YMC-Triart brochure.

Fig.: Separation of oligonucleotide crude by using YMC-Triart C18 UHPLC column.

Essential guideline for robust HIC methods for proteins and mAbs

A recently published protocol paper summarises how to set up robust HIC methods. The paper focuses on the analysis of intact proteins and mAbs. Prof. Eeltink’s group (University of Brussels/VUB) present helpful and detailed aspects especially for:

  • How to set-up and maintain the system to achieve robust performance
  • The effect of sample-preparation conditions on sample breakthroughs
  • Run-to-run repeatability for 5 different columns obtained from 3 different column lots

The samples analysed are a mixture of intact proteins or monoclonal antibodies. The column of choice is YMC’s BioPro HIC HT column.

The full paper is freely accessible! You want know more about the applied BioPro HIC HT columns? Download the YMC BioLC catalogue below.

New Application Note

Analysis of denaturated and non-denaturated siRNA via IP-RP and AEX

siRNAs are a powerful tool for genetic analysis and treatment of metabolic diseases. siRNAs can be analysed as duplex by HPLC under non-denaturing conditions to control duplex formation and the amount of excess single strands. In addition, non-hybridized single stranded RNA can be studied under denaturing conditions. In both cases, IP-RP and AEX are the most commonly used methods.

In this Application Note the analysis of a siRNA duplex under denaturing and non-denaturing conditions is shown. A bioinert YMC-Accura Triart Bio C18 UHPLC column is used in IP-RP mode, while a BioPro IEX QF column is used in AEX mode.

To obtain a full copy of this Application Note, please see below.

For more information on YMC-Accura Triart and BioPro IEX columns check out the YMC-BioLC catalogue below.

Fig.: Separation of siRNA duplex under denaturing conditions by using a YMC-Triart C18 UHPLC column (blue) and a BioPro IEX QF column (green).