siRNAs are a powerful tool for genetic analysis and treatment of metabolic diseases. siRNAs can be analysed as duplex by HPLC under non-denaturing conditions to control duplex formation and the amount of excess single strands. In addition, non-hybridized single stranded RNA can be studied under denaturing conditions. In both cases, IP-RP and AEX are the most commonly used methods.
In this Application Note the analysis of a siRNA duplex under denaturing and non-denaturing conditions is shown. A bioinert YMC-Accura Triart Bio C18 UHPLC column is used in IP-RP mode, while a BioPro IEX QF column is used in AEX mode.
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For more information on YMC-Accura Triart and BioPro IEX columns check out the YMC-BioLC catalogue below.