Everyone working with peptides knows that the purification is a challenging task! Due to the complexity of the impurity profile, standard methods may not be sufficient to achieve the criteria for purity and recovery. In these cases, additives such as salts can be highly beneficial.
This expert tip shows that the addition of salt to the mobile phase can massively improve the separation in an insulin purification process.
With the additive, the purity and recovery easily fulfilled the required process criteria! Download here the full expert tip.