Sensitive LC-MS/MS Analysis of Sphingophospholipids

In LC analyses of sphingophospholipids, such as sphingosine-1-phosphate(S1P) and ceramide-1-phosphate (C1P), phosphate groups in these molecules causes can cause significant peak tailing. Therefore, loss of sensitivity and reproducibility can be observed.

Gowda et al. published a sensitive LC-MS/MS application in the “Analytical and Bioanalytical Chemistry” journal. The application nicely shows that by implementing a metal free column hardware any tailing is avoided.

 
Download the application note with the full method details here.
 
Please download the YMC-Triart brochure to receive more information on YMC-Triart metal-free columns.

 

 

Efficient Peptide Purification

How to obtain an efficient and reliable purification of peptide pharmaceuticals?

In order to achieve commercial success with any API, consideration of the production costs is essential. Whenever a chromatographic step is involved, purity, yield and throughput are the centre of attention. This Peer Review article summarises all the modern purification techniques comprehensively; definitely a helpful resource for your development work.

You are guided from initial process development, through the stages for optimisation and to the final scale up. Using liraglutide as the prime example, selection of a suitable stationary phase, as well as optimal method conditions are addressed.

For more information we invite you to see our website here.

YMC Europe Inhouse Seminars at your Site

 

You can´t attend a seminar on our site?
You want a number of colleagues to benefit from our seminars?
You expect a special seminar tailored to your needs?
 
Make use of our on site seminars!
Choose from our seminar program or select your own topics. The overview, which you can find here, will assist you. You can arrange the date, location and number of attendees flexibly with us.
We will be happy to advise you!
 
Your benefits at a glance:

  • Topics and duration by agreement
  • Date and location by agreement
  • Flexible number of attendees
  • Fixed cost

 

On Site Seminars YMC Europe

 

 

Go back