When working with biomolecules, loss of analytes due to absorption by the metal hardware of the column is a well-known issue. Biomolecules with phosphate groups such as phosphorylated peptides tend to adhere strongly to the column surface. The use of bioinert column hardware is a great advantage and enables:
- higher recovery
- better peak shapes
- greater reproducibility
In this Application Note the analysis of phosphorylated peptides using a bioinert YMC-Accura Triart column is compared to a standard stainless steel column.