In method transfer from HPLC to UHPLC methods, stationary phase particle size, column length and internal diameter usually change. But how do you ensure to still work at the optimal flow rate? Perhaps you’re scaling up from an analytical method to a semi-preparative one. But how do you keep the same retention times?
This expert tip provides a quick reference of common combinations for column dimensions and suitable method parameters to help chromatographers in method transfer, scaling, optimisation and troubleshooting.
Download this useful overview and evaluate if you can see potential for optimisation in your methods!