High resolution CEX analysis of commercial monoclonal antibodies

Are you working with monoclonal antibodies (MAbs) and want to characterise them?

Cation exchange chromatography (CEX) is perceived to be the gold standard for the charge sensitive characterisation of MAbs. Acidic and basic variants caused by chemical or enzymatic modifications can be separated from the main isoform of the MAb and have to be critically evaluated.
 
In this application note, four therapeutic MAbs of different species, isotypes and isoelectricpoint (pI) together with their variants were analysed using pH and salt gradient modes. YMC’s non-porous BioPro IEX SF column was used to characterise Natalizumab (Tysabri®), Cetuximab (Erbitux®), Adalimumab (Humira®) and Denosumab (Prolia®; XGEVA®).


You can download the application note with the full method details.
 
Please download YMC’s BioLC catalogue or visit our homepage to receive more information on YMC’s non-porous and porous BioPro IEX columns.

 

Figure: pH gradient separations of the four monoclonal antibodies
Figure: pH gradient separations of the four monoclonal antibodies

Which LC mode is more suitable for different
ADC-types?

 

Are you working with different ADCs?

Do you use HIC or RP for their characterisation?

This Technical Note describes three different ADC conjugation types and demonstrates how RP is gaining more importance in the analysis of ADCs and their DAR species.

HIC is the standard technique for the analysis of ADCs and for the determination of drug-to-antibody ratio (DAR). The new generation of ADCs can also be analysed via RP which offers advantages such as direct MS coupling and significantly higher resolution.

Learn more about the use of RP analysis of ADCs and get your free copy of the technical note

 

 

 

New: Covid-19 Information sources by YMC

  • Helpful application notes covering substances used in current Covid-19 research.
  • Application notes for RNA separations used in several approaches for vaccine production.
  • A selection of publications on the substances used in Covid-19 treatment. These clearly show which YMC column has been used in the publication or the YMC column recommended for the method described.

 
You can directly access YMC’s Covid-19 resource page following.

 

 

BioLC application collection with LCGC

650

Produced in conjunction with LCGC Europe, we have released a selection of recent application notes for different biomolecules highlighting methods for antibodies, ADCs, proteins, and oligonucleotides!

All available BioLC modes using columns from YMC, namely RP, SEC, IEX or HIC alongside special techniques such as MicroLC or LC-MS are covered.

 
You can access the application notes with the full method details.
 
Please download YMC’s BioLC catalogue or visit our homepage to receive more information on our range of BioLC applications.

 

 

We value your experience – why not share it?

 

YMC values your opinion and experience! As an experienced user you might want to tell us and other users of your experiences with your YMC columns. In addition, you have the chance to win an amazon voucher periodically.
 
Share your opinion of your YMC BioLC columns by simply clicking on one of the links below and you will be guided through a simple 4-step form:
 

 
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