YMC i-Mail July 2023

New Expert Tip

Accurate Bio-(U)HPLC Results

The analysis of sensitive compounds containing e.g. phosphate or multiple carboxylate groups is a great challenge. This is due to non-specific adsorption on metallic surfaces often resulting in peak tailing, reduced recovery and/or shifts in retention time.

This expert tip presents various methods that easily achieve reproducible results:

  • Passivation of the system and column
  • Additives to the mobile phase
  • Sample pre-conditioning of the system and column
  • (Bio)inert system and column

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Application Note

Outstanding steric selectivity of YMC-Triart C18 ExRS columns for the analysis of chlorophenols

The greatest challenge in monitoring highly toxic chlorophenols in the environment, is their structural similarity. Compounds of this group often differ only in the amount and/or position of the chlorine substituents. Separation by HPLC requires high steric selectivity of the stationary phase.

This required selectivity is provided by YMC-Triart C18 ExRS columns due to their high carbon content and the small pore size of the stationary phase, which enables steric recognition.

In this Application Note baseline separation of seven chlorophenols is achieved using a YMC-Triart C18 ExRS column.

Figure: Baseline separation of seven chlorophenols using YMC-Triart C18 ExRS.