The separation of oligonucleotides for preparative purification can be a big challenge. The use of IP-RP conditions is highly advantageous for the development of efficient separation methods but requires special techniques. This new application note shows the principle and benefits of ion-pairing reagents for the separation of oligos via IP-RP.

For the purification of oligonucleotides using IP-RP methods, YMC offers several ideally suited phases. The YMC-Triart materials provide additional features such as increased lifetime and an extended pH range.

Learn more about oligo purification via IP-RP and the YMC stationary phases in the new application note.


 

High performance stationary phase and the efficient and reliable packing of these materials into the chromatography columns are the key for productive processes.

With this new and handy overview, you’ll get practical support for the packing of YMC’s hybrid-silica and silica-based materials into your DAC-columns, including important considerations and calculations.

An additional practical example: Packing of YMC-Triart Prep into a 250 x 50 mm ID column including qualification!