The separation of oligonucleotides for preparative purification can be a big challenge. The use of IP-RP conditions is highly advantageous for the development of efficient separation methods but requires special techniques. This new application note shows the principle and benefits of ion-pairing reagents for the separation of oligos via IP-RP.
For the purification of oligonucleotides using IP-RP methods, YMC offers several ideally suited phases. The YMC-Triart materials provide additional features such as increased lifetime and an extended pH range.
Learn more about oligo purification via IP-RP and the YMC stationary phases in the new application note.