This question often arises when thinking about equilibration of the column after a gradient run. If the (re)equilibration time is too short, the risk of non-reproducible results occurs. If it is too long, time and solvents are wasted.
In this Expert Tip, you will learn how to estimate the optimum equilibration volume by calculating the column volume. In addition, a practical example is used to demonstrate the effects of different equilibration volumes. For more information about equilibration, you can download the Expert Tip here.