Fast UHPLC analyses of small peptides

The risk to human health from the well-known herbicide glyphosate is still unclear, therefore the analysis of glyphosate and its main metabolite aminomethylphosphonic acid (AMPA) is highly relevant.

Due to their high polarity, the substances need to be derivatised in order to achieve an improved retention on a C18 column. In this application note, the UHPLC/MS analysis of FMOC-derivatised glyphosate and AMPA in the range of 30-800 ng/L is shown. The derivatisation is performed according to DIN ISO 16308. The challenging pH value of 9.5 can easily be used due to the highly robust hybrid silica particles of YMC-Triart. The YMC-Triart C18 column provides sharp peaks over the entire concentration range.

You can download a copy of the full application note here.

For further information on YMC-Triart columns, please visit our homepage or download the YMC-Triart brochure.


Fig.: Extracted ion-chromatogram at m/z=332 for glyphosate-FMOC and AMPA-FMOC with a concentration of 800 ng/L.

New Application Notes

New chiral and achiral Application Notes have been developed using YMC’s CHIRAL ART and YMC-Triart columns.

The nucleoside cytostatic agent nelarabine (Atriance®) is separated from its other enantiomer applying a CHIRAL ART Cellulose-SC column. This column is very robust due to its immobilised chiral polysaccharide selector.

8-OHdG (8-hydroxy-2'-deoxyguanosine) is a critical biomarker for oxidative stress and carcinogenesis. 8-OHdG and related compounds can either be separated in RP-mode using a YMC-Triart C18 UHPLC column or with an orthogonal HILIC method using a YMC-Triart Diol-HILIC UHPLC column. Both methods use MS-compatible conditions.

More information on YMC’s CHIRAL ART polysaccharide columns or YMC-Triart columns can be found on our new homepage.


Fig: Separation of 8-OHdG and related compounds using a YMC-Triart C18 UHPLC column.