The risk to human health from the well-known herbicide glyphosate is still unclear, therefore the analysis of glyphosate and its main metabolite aminomethylphosphonic acid (AMPA) is highly relevant.
Due to their high polarity, the substances need to be derivatised in order to achieve an improved retention on a C18 column. In this application note, the UHPLC/MS analysis of FMOC-derivatised glyphosate and AMPA in the range of 30-800 ng/L is shown. The derivatisation is performed according to DIN ISO 16308. The challenging pH value of 9.5 can easily be used due to the highly robust hybrid silica particles of YMC-Triart. The YMC-Triart C18 column provides sharp peaks over the entire concentration range.
You can download a copy of the full application note here.
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