Oligonucleotides are separated very well at high temperatures in combination with high pH, ​​e.g. with HFIP-TEA at pH>8 and 90°C. But the ultimate solution for separating oligonucleotides are bio-inert columns with YMC-Triart Bio C18 as the stationary phase. The recently introduced YMC-Accura Triart Bio C18 columns are equipped with a bio-inert coating. The applied coating enables immediate, high-precision results from the 1st injection - without the need for sample conditioning!

Of course, YMC-Triart Bio C18 columns are stable up to 90°C and in combination with a pH range of pH=1-9.

If you are interested in characterising oligonucleotides, this application note, which shows two methods – RP and AEX – to analyse EGFP mRNA, is highly recommended. Applying the RP analysis excellent peak shapes and recoveries are achieved using a bioinert YMC-Accura Triart Bio C4 column at pH 7 and 80 °C. AEX, as alternative approach, also gives exceptionally sharp peaks using a BioPro IEX QF column with a run time of only 10 minutes!
You can download the full method details here!

On November 21^st and 22^nd LCGC’s virtual symposium Biopharmaceutical Analysis: The State of The Art took place. Did you miss it? No worries!

All talks are available on demand. Besides interesting talks from selected scientists watch YMC’s talk Guideline for modern oligonucleotide RP-(U)HPLC separations and learn more about:

• Basic requirements for the stationary phase for a successful IP-RP separation of oligonucleotides
• Screening optimum (LC-MS compatible) conditions for diverse oligonucleotides

Hardware effects and other impacts on the analysis and how to receive reproducible results