New App Note: HIC-MS analysis of ADCs

Are you characterising ADCs via HIC?
Do you want to determine the DAR using MS without the need for peak isolation?

In this application note the drug-to-antibody ratio (DAR) of a cys-linked ADC-mimic is determined using online native HIC-MS. Using the innovative BioPro HIC BF column and a direct coupling to the mass spectrometer even degraded payloads can be detected. The application also demonstrates how to overcome the high salt concentrations which are necessary for HIC methods.

To get more details download the application note.

Please visit our homepage to receive more information on YMC’s HIC columns for BioLC.

How to develop an IEX method for commercial MAbs using a Dial-a-mix method

Do you analyse MAbs using IEX?
Do you want to speed up the method development?

This technical note describes how to find the optimum pH and salt gradient for the IEX analysis of antibodies. Commercial MAbs as Cetuximab (Erbitux®), Bevacizumab (Avastin®) and Rituximab (MabThera®) were used. Screening with a dial-a-mix method and a quaternary HPLC pump helps to adjust buffer pH online. This type of screening allows very fast method development – results can be achieved overnight.

For the complete instructions download the technical note.

Please visit our homepage to receive more information on YMC’s IEX columns.

Faster analysis of leuprorelin acetate using YMC-Triart Bio C18

A method to analyse the peptide leuprorelin (e.g. Enantone®), a synthetic analogue of gonadotropin-releasing hormone (GnRH), is described in the Japanese Pharmacopeia (JP) using a C18 column.

In this application note YMC-Triart Bio C18 is used, which has a lower surface area and a lower hydrophobicity due to its 300 Å pore size. By applying a flow rate of 0.75 mL/min the retention time criterion (41-49 min) of the JP is matched.

Using YMC-Triart Bio C18 the separation could be speeded up by applying a higher flow rate of e.g. 1 mL/min which results in a retention time of about 34 min. YMC-Triart Bio C18 is also able to separate leuprorelin from its degradation products caused by alkaline degradation.

Download the application note with the full method details here.

Please download YMC’s BioLC catalogue or visit our homepage to receive more information on YMC’s RP columns for BioLC.