The capsid of adeno-associated viruses (AAVs) consists of three viral proteins (VPs, 62-90 kDa). Alterations in the dedicated protein composition can influence the vector's potency and increase the risk of immunogenicity.
To reliably determine the ratio of the three proteins VP1, VP2 and VP3, reversed phase liquid chromatography (RP-LC) coupled to mass spectrometry (MS) is a viable option. Only in combination with bioinert column hardware can a good separation of all three proteins be achieved. When using stainless steel column hardware, the negatively charged capsid surface interacts with the column body, which results in a decrease in resolution.
The advantage of using bioinert YMC-Accura Triart Bio C4 columns when analysing AAV capsid proteins: sharp peaks and excellent resolution.
This Technical Note demonstrates the advantage of using a bioinert YMC-Accura Triart Bio C4 column for the analysis of AAV capsid proteins.
For more information on YMC-Accura Triart columns please look here or check out the YMC-Accura Triart flyer.