New chiral RP Application Note: DL-norgestrel / levonorgestrel

While the racemate of the progestin DL-norgestrel has been marketed for several years, today the enantiomer levonorgestrel is used. Even though levonorgestrel can be prepared by asymmetric synthesis, it is very important to separate and identify the two enantiomers from each other and/or quantify them.

This new application note demonstrates how the enantiomers can reliably be separated using YMC’s immobilised CHIRAL ART Cellulose-SC column in RP mode. These immobilised CHIRAL ART columns are very robust and allow for use in RP mode without any restrictions in regard of eluents or sample solvents.

More information on YMC’s CHIRAL ART polysaccharide columns can be found on our new homepage or in the YMC Chiral brochure.


Fig: Separation of L- (1) and D-norgestrel (2) using a CHIRAL ART Cellulose-SC column.

New Expert Tip: How to improve the analysis of hydrophobic compounds

Very hydrophobic compounds require a special treatment in RP chromatography. They usually have extended elution times on C18 columns or even worse: they only elute partially or not at all. Finding the appropriate mobile phase and sample solvent also needs to be thoroughly optimised. In summary, hydrophobic analytes demand a careful method development. Download your copy of this new Expert Tip and find out how to improve the analysis of hydrophobic compounds by   

  • Selecting the most appropriate stationary phase
  • Optimising the mobile phase and
  • Checking the solubility of the sample.

You can access more valuable technical resources by registering for YMC’s new Expertise Portal!



Fig.: Separation of vitamin D2 and D3 using YMC-Triart C18 and YMC-Triart C18 ExRS columns.

Looking for applications of polar pesticide analyses?

Obtaining reasonable retention of polar pesticides in reversed phase chromatography is a well-known challenge. Special stationary phases are required: in addition to providing a polar selectivity, they have to be stable using 100% aqueous conditions or at least very low amounts of organic in the mobile phase. Another option to increase the retention is to derivatise the polar analytes making them more hydrophobic.
Using YMC columns, the analysis of polar pesticides is no longer an obstacle. In the YMC application database, you will find numerous applications including:

Discover more examples of polar pesticide analyses using the YMC application database.



Fig.: Separation of four hydrophilic dipeptides.