Very hydrophobic compounds require a special treatment in RP chromatography. They usually have extended elution times on C18 columns or even worse: they only elute partially or not at all. Finding the appropriate mobile phase and sample solvent also needs to be thoroughly optimised. In summary, hydrophobic analytes demand a careful method development. Download your copy of this new Expert Tip and find out how to improve the analysis of hydrophobic compounds by
- Selecting the most appropriate stationary phase
- Optimising the mobile phase and
- Checking the solubility of the sample.
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