SFC Applications with Versatile and Safe YMC Columns
SFC columns are the heart of every SFC application. The most important requirement is the consideration of all aspects of safety. Therefore, you can buy specifically tested SFC columns in the YMC portfolio, which ensure your lab safety.
Moreover, YMC columns have been tested by an independent institute. The AFIN-TS Institute (Analytisches Forschungsinstitut für Non-Target Screening GmbH) tested the selectivity as well as the separation efficiency in SFC mode of YMC-Triart Diol (1.9 µm, 12 nm).
These independent results confirm:
- Versatile application possibilities
- Fast separations
Several substances with different logD values were tested to verify the column selectivity of YMC-Triart Diol. The goal was to achieve the best separation in the shortest time. All substances could be separated and detected, which proves the versatility of YMC-Triart Diol.
The results also show very consistent behaviour for all compounds at each backpressure. Furthermore, a very wide flow rate range can be used without significant effects on the efficiency, which accurately corresponds to the behaviour in LC mode.
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Equilibration – As much as necessary, as little as possible
During equilibration and rinsing a column, the following question often arises: “For how long do I have to flush the column?” If the (re)equilibration time is too short, there is a risk of non-reproducible results (e.g. retention time shift). If in doubt a longer duration of flushing should be favoured. But if the rinse time is too long, time and, especially for preparative methods, solvents are wasted.
A YMC-Triart C8 column (TO12S03-1546WT, column volume 2.5 mL) was rinsed with 50 mL of acetonitrile before each run. A plant extract was injected under four different conditions:
- without equilibration
- equilibration with 1 column volume
- equilibration with 5 column volumes
- equilibration with 10 column volumes
It is obvious, without sufficient equilibration the retention time shifts to shorter times. When using 5 or 10 column volumes, the results of this application are reproducible. Equilibration with 5 column volumes is most efficient.
For detailed and further information, e. g. on how to calculate the column volume, download the technical document here.