Method Development Strategies

Whitepaper for

LC Method Development Strategies

The objectives for method developers in preparative LC are:

Purification and isolation of a target compound with a maximum sample amount in the shortest cycle time under cost pressure. Whereas isolation focuses on the scale up of mg- to g-units, the production scale needs to achieve up to kilograms or even tons.

Learn more about LC Method Development Strategies in our whitepapers.

Steps of the linear scale-up

The most comprehensive approach for method development in
preparative LC is the so-called linear scale-up, which consist of:

 

  • Method development at analytical scale
  • Loadability studies on analytical scale
  • Scale-up to preparative process
  • Regulatory Affairs Support

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