This Technical Note based on the publication of Tsalmpouris et al. presents AEX as reliable and efficient alternative to commonly used fluorescence-based assays for determining the encapsulation efficiency (EE) of mRNA in lipid nanoparticles (LNPs).

This workflow not only increases analytical performance but also yields additional structural insights into LNP composition – delivering added value for advanced pharmaceutical analysis.

The use of bioinert-coated YMC Accura BioPro IEX QF columns further prevents non-specific adsorption, resulting in stable peak areas and reliable quantification.

Access all experimental details and chromatographic conditions in the full Technical Note.

Hydrophilic interaction chromatography (HILIC) provides an orthogonal alternative to established ion pair reversed-phase (IP-RP) methods – particularly in applications involving mass spectrometry detection.

HILIC offers clear advantages when analysing hydrophilic modifications and performing MS-based detection. It is not only able to separate oligonucleotides by length it is also suitable for the separation of different modifications like phosphorothioated or methoxylated oligonucleotides.

All conditions, chromatograms, and conclusions are detailed in the current Expert Tip.