The development of successful mRNA therapeutics begins with reliable quality control. Our new application note demonstrates how the bioinert YMC Accura Triart Bio C18 column enables high-resolution separation of capped, partially capped and uncapped mRNA species, even where structural differences are minimal. When combined with LC-MS, users gain a powerful solution for the precise determination of capping efficiency and the identification of critical impurities.

A recent publication by Rockel et al. demonstrates – for the first time – the isotope-specific analysis of the water-soluble vitamins B5 and B9 in complex dietary supplement and beverage matrices. The innovative Heart-Cutting 2D LC-IRMS method opens up new possibilities for authenticity and origin analyses and expands the scope of application of LC-IRMS technology. The successful implementation of the two-dimensional LC approach is supported by the high chromatographic performance of the YMC Triart C18 column, which enables reliable separation of the target analytes even in challenging matrices.
  
Highlights of the study:

✔   The first isotope-specific analysis of B vitamins in complex supplement and beverage matrices
✔   Extension of LC-IRMS applicability to polar and water-soluble analytes
✔   High precision with a repeatability of ≤ 0.13 ‰
✔   Efficient separation of the target analytes using the YMC-Triart C18 column

Varying sequence lengths, chemical modifications and complex impurity profiles require precisely tailored chromatographic strategies.

Two new Expert Tips assist in the selection of suitable chromatographic conditions for the analysis of therapeutic oligonucleotides. These Expert Tips facilitate method development for IP-RP and AEX applications. Tables, selectivity overviews and methodological guidelines provide a sound basis for selecting suitable ion-pair reagents, buffer systems, temperatures and stationary phases.

Download Expert Tips now:
Expert Tip IP-RP and Expert Tip AEX