The characterisation of therapeutic oligonucleotides is becoming increasingly challenging, particularly when structurally similar impurities must be reliably detected and identified.

Our latest Application Note demonstrates, using nusinersen as an example, how these compounds can be reproducibly separated and unambiguously characterised by IP-RP LC-MS. The study systematically investigates the influence of key method parameters, including temperature, mobile phase composition, and ion-pairing reagents.

Learn how:

   ✔     to reproducibly separate nusinersen and structurally related impurities
   ✔     temperature, eluent and IP reagents affect separation performance
   ✔     to use LC-MS for reliable compound identification
   ✔     to apply this approach to other therapeutic oligonucleotides

The method is based on the bioinert YMC Accura Triart Bio C18 wide-pore column.

Carotenoids are among the most challenging analytes in RP-HPLC. In particular, isomeric compounds are often difficult to separate using conventional C18 phases. Our latest HPLC poster demonstrates how the YMC Carotenoid C30 phase provides significant advantages.

Find out how:

  ✔     isomers can be reliably separated
  ✔     dietary supplements can be reliably analysed
  ✔    the C30 phase compares with C18, phenyl and PFP

Varying sequence lengths, chemical modifications and complex impurity profiles require precisely tailored chromatographic strategies.

ISC 2026 is just around the corner, and we are looking forward to five exciting days of scientific exchange, fresh ideas, and inspiring conversations. From September 6-10, we look forward to reconnecting with familiar faces, meeting new contacts, and presenting our latest developments both at our exhibition booth and through our scientific contribution.

If you are attending ISC, we invite you to visit us at our booth. Take the opportunity to discuss current research, method development, and practical applications with our team