The precise separation of sgRNA and closely related by-products is analytically challenging, particularly due to minimal length differences and complex secondary structures. The wide-pore YMC Accura Triart Bio C18 column with bioinert hardware enables high-resolution separation, even of structurally similar impurities. Benefit from the approach developed by the Belgian CRO Quality Assistance, offering reproducible results, minimised carry-over, and stable performance even at low concentrations.

If you want to characterise mRNA samples quickly and reliably, the new 2D-LC approach by Carstensen et al. provides a powerful solution. By combining IPAX and IP-RP, high-resolution separation of mRNA fragments and clear differentiation of complex structures can be achieved. In particular, differences in sequence and poly(A) tail structure become visible, enabling precise assessment of mRNA quality.

Download the full Technical Note now to gain practical insights into this innovative method.