Available phases are:
BioPro Ion Exchange Columns are characterised by:
- Non-porous or porous
particles - High loadability and
recovery - Very high resolution
- High dynamic binding capacity
- Long term stability
- Excellent lot-to-lot
reproducibility
Applications
| Application | Column | Compound name | |
| DNA fragments | BioPro IEX QF | DNA fragments | |
| Lambda DNA Hind Ⅲ/EcoRⅠ restriction fragments | BioPro IEX QF | Lambda DNA Hind III/EcoR I digest (130-23, 130 bp) | |
| Monoclonal antibody (MAb) | BioPro IEX SF | Humanized monoclonal IgG1 | |
| Peptide mapping | BioPro IEX QA | Peptide mapping | |
| Plasmid pBR322 and pBR322 Hae III restriction fragments | BioPro IEX QF | Plasmid pBR322 Hae III digest (8-587 bp) Plasmid pBR322 (4361 bp) | |
| Proteins in human serum | BioPro IEX QA | IgG Transferrin Albumin | |
| Proteins | BioPro IEX QA | Ovalbumin Trypsin inhibitor (soybean) | |
| Synthetic oligonucleotides (Single-strand DNA) | BioPro IEX QF | 5’-TCATCACACTGAATACCAAT-3’ (20 mer) 5’-GTCATCACACTGAATACCAAT-3’ (21 mer) | |
| Synthetic oligonucleotides (Single-strand RNA) | BioPro IEX QF | 5’-U(M)C(M)A(M)U(M)C(M)A(M)C(M)A(M)C(M) U(M)G(M)A(M)A(M)U(M)A(M)C(M)C(M)A(M)A(M)U(M)-3’ (20 mer) 5’-G(M)U(M)C(M)A(M)U(M)C(M)A(M)C(M)A(M)C(M)U(M) G(M)A(M)A(M)U(M)A(M)C(M)C(M)A(M)A(M)U(M)-3’ (21 mer) |
Specifications
|
|
BioPro IEX QA
|
BioPro IEX SP
|
BioPro IEX QF
|
BioPro IEX SF
|
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|
Base Material
|
hydrophilic polymer (polymethacrylate)
|
|||||
|
Particle Size [µm]
|
5 µm
|
3, 5 µm
|
||||
|
Pore Size [nm]
|
100
|
non-porous
|
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pH range
|
2.0 - 12.0
|
|||||
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Temperature range
|
4 - 60°C
|
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Phase Details
BioPro IEX QA/SP
|
(formerly)
|
BioPro IEX QA
(YMC-BioPro QA)
|
BioPro IEX SP
(YMC-BioPro SP)
|
||
|
Matrix
|
Hydrophilic polymer (polymethacrylate)
|
|||
|
Particle Size
|
5 µm
|
|||
|
Pore Size
|
100 nm / 1000 Å
|
|||
|
Charged group
|
-CH2N+(CH3)3
(strong anion exchanger)
|
-(CH2)3SO3-
(strong cation exchanger)
|
||
|
Counter ion
|
Cl-
|
Na+
|
||
|
Ion-exchange capacity
|
0.075-0.100 meq/ml-resin
|
0.070-0.095 meq/ml-resin
|
||
|
Dynamic binding capacity
|
110-150 mg BSA/ml-resin
|
70-100 mg human IgG/ml-resin
|
||
|
pH range
|
2.0 - 12.0
|
|||
|
Temperature range
|
4 - 60°C
|
|||
|
Column hardware
|
PEEK
|
|||
|
Pressure limit
|
2.5 - 3.5 MPa (360 - 510 psi)
|
|||
BioPro IEX QF/SF
|
(formerly)
|
BioPro IEX QF
(YMC-BioPro QA-F)
|
BioPro IEX SF
(YMC-BioPro SP-F)
|
|
Matrix
|
Hydrophilic polymer (polymethacrylate)
|
|
|
Particle Size
|
3, 5 µm
|
|
|
Pore Size
|
Non-porous
|
|
|
Charged group
|
-CH2N+(CH3)3
(strong anion exchanger)
|
-(CH2)3SO3-
(strong cation exchanger)
|
|
Counter ion
|
Cl-
|
Na+
|
|
Ion-exchange capacity
|
0.075-0.110 meq/ml-resin
|
0.230-0.290 meq/ml-resin
|
|
Dynamic binding capacity
|
12-20 mg BSA/ml-resin
|
10-18 mg human IgG/ml-resin
|
|
pH range
|
2.0 - 12.0
|
|
|
Temperature range
|
4 - 60°C
|
|
|
Column hardware
|
PEEK
|
|
|
Pressure limit
|
3 µm: 25 MPa (3,625 psi)
5 µm: 6 - 12 MPa (870 - 1,740 psi)
|
|
The non-porous BioPro IEX particles offer high efficiency, exceptional resolution, and low operating pressures.
Technical Library
| Title | Document Type | Language | Download |
 (U)HPLC analysis of siRNA under denaturing and non-denaturing conditions using IP-RP and AEX |
Application Note | EN | |
| Analysis of mRNA coupled to enhanced green fluorescent protein by RP and IEX |
Application Note | EN | |
| Charge heterogeneity characterisation of an IgG4-based mAb using AEX coupled to MS |
Application Note | EN | |
| HPLC Analysis of Oligonucleotides with BioPro IEX QF |
Application Note | EN | |
| High resolution analysis of monoclonal antibodies using the cation exchange column BioPro IEX SF |
Application Note | EN | |
| MS compatible charge variant analysis of 28 commercial MAbs by CEX |
Application Note | EN | |
| Native AEX-MS analysis of IgG4-based mAbs |
Application Note | EN | |
| Native online SCX-MS analysis of monoclonal antibodies using YMC's BioPro IEX SF |
Application Note | EN | |
| Optimisation of oligonucleotide separations using YMC´s non porous anion exchanger BioPro IEX QF |
Application Note | EN | |
| Optimisation of phosphorothioate oligonucleotide separation using BioPro IEX QF |
Application Note | EN | |
 YMC Oligonucleotide Columns |
Brochure | EN | |
 BioPro IEX Columns Care and Use |
Care and Use Instructions | EN | |
 YMC Biochromatography Columns |
Catalogue | EN | |
| YMC Biochromatography Columns (HQ) |
Catalogue | EN | |
| YMC Biochromatography Columns - IEX Columns Chapter |
Catalogue | EN | |
 Minimising carryover in oligonucleotide analysis by AEX |
Expert Tip | EN | |
 Analytical Stationary Phases for BioLC from YMC |
Overview | EN | |
 A Stability-Indicating Method for Separation of Charge Variant Impurities of a PEGylated Protein by CEX-HPLC |
Poster | EN | |
| An Evaluation of YMC’s Strong Cation-Exchange Stationary Phase for the Analysis of Charged Isoforms of a Commercially Available Antibody Drug |
Poster | EN | |
| Oligonucleotide purification using anion exchange (AEX) and ion-pair reversed phase chromatography (IP-RP LC) |
Poster | EN | |
| Optimisation of oligonucleotide separations on ion exchange chromatography |
Poster | EN | |
| The Principles of BioLC & YMC BioLC Phase Overview |
Poster | EN | |
| Use of Small Particle YMC SEC and IEX Materials for Improved Characterization of Monoclonal Antibodies |
Poster | EN | |
 Characterisation of charge heterogeneity of therapeutic monoclonal antibodies via IEX-MS using BioPro IEX SF from YMC |
Product Information | EN | |
| YMC Alternatives to Existing BioLC Columns |
Product Information | EN | |
 Advantages of strong cation exchangers vs. weak cation exchangers |
Technical Note | EN | |
| High temperature stability of BioPro IEX columns |
Technical Note | EN | |
| How to optimise your oligonucleotide analysis |
Technical Note | EN | |
| Utilisation of AEX-MS for charge variant analysis of IgG4-based mAbs! |
Technical Note | EN | |
 The Utility of a Cation Exchange Column for Successful Charge-Variant Analysis of PEGylated Proteins |
Whitepaper | EN |
Ordering Information
Please login to see the prices.
- 2-3 days
- 1-2 weeks
- 3-4 weeks
| Product | Description | Price | Stock |
| QAA0S05-0346WP | BioPro IEX QA, PEEK HPLC column (4.6 mm ID), 100 nm, S-5 µm, 30 x 4.6 mm | -1919 CHF1565 € | |
| QAA0S05-0546WP | BioPro IEX QA, PEEK HPLC column (4.6 mm ID), 100 nm, S-5 µm, 50 x 4.6 mm | -1919 CHF1565 € | |
| QAA0S05-1046WP | BioPro IEX QA, PEEK HPLC column (4.6 mm ID), 100 nm, S-5 µm, 100 x 4.6 mm | -2068 CHF1687 € | |
| QAA0S06-1010WT | BioPro IEX QA, semi-preparative HPLC Column (20 mm ID), S-06 µm, 100 x 20 mm | -11670 CHF9519 € | |
| QAA0S06-1020WT | BioPro IEX QA, semi-preparative HPLC Column (20 mm ID), S-06 µm, 100 x 20 mm | -29057 CHF23701 € | |
| QF00S03-0346WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 30 x 4.6 mm | -2130 CHF1737 € | |
| QF00S03-0546WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 50 x 4.6 mm | -2564 CHF2091 € | |
| QF00S03-1046WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 100 x 4.6 mm | -2912 CHF2375 € | |
| QF00S03-1546WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 150 x 4.6 mm | -3067 CHF2502 € | |
| QF00S05-0346WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 30 x 4.6 mm | -1596 CHF1302 € | |
| QF00S05-0546WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 50 x 4.6 mm | -1867 CHF1523 € | |
| QF00S05-1002WT | BioPro IEX QF, non-porous, Narrowbore HPLC column (2.0 mm ID), S-5 µm, 100 x 2.0 mm | -2776 CHF2264 € | |
| QF00S05-1046WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 100 x 4.6 mm | -2119 CHF1728 € | |
| QF00S05-1546WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 150 x 4.6 mm | -2733 CHF2229 € | |
| QF00S06-1020WT | BioPro IEX SF, non-porous, semi-preparative HPLC Column (20 mm ID), S-6 µm, 100 x 20 mm | -27760 CHF22643 € | |
| QF00S06-2546WP | BioPro IEX QF, non-porous, PEEK HPLC column (4.6 mm ID), S-6 µm, 250 x 4.6 mm | -3178 CHF2592 € | |
| SF00S03-0346WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 30 x 4.6 mm | -2130 CHF1737 € | |
| SF00S03-0546WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 50 x 4.6 mm | -2564 CHF2091 € | |
| SF00S03-1046WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 100 x 4.6 mm | -2912 CHF2375 € | |
| SF00S03-1546WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-3 µm, 150 x 4.6 mm | -3067 CHF2502 € | |
| SF00S05-0346WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 30 x 4.6 mm | -1596 CHF1302 € | |
| SF00S05-0502WT | BioPro IEX SF, non-porous, Narrowbore HPLC column (2.0 mm ID), S-5 µm, 50 x 2.0 mm | -2427 CHF1980 € | |
| SF00S05-0546WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 50 x 4.6 mm | -1867 CHF1523 € | |
| SF00S05-1002WT | BioPro IEX SF, non-porous, Narrowbore HPLC column (2.0 mm ID), S-5 µm, 100 x 2.0 mm | -2776 CHF2264 € | |
| SF00S05-1008WT | BioPro IEX SF, non-porous, Classical Analytical HPLC column (8.0 mm ID), S-5 µm, 100 x 8.0 mm | -6101 CHF4976 € | |
| SF00S05-1046WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 100 x 4.6 mm | -2119 CHF1728 € | |
| SF00S05-1546WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-5 µm, 150 x 4.6 mm | -2733 CHF2229 € | |
| SF00S06-1010WT | BioPro IEX SF, non-porous, semi-preparative HPLC Column (10 mm ID), S-6 µm, 100 x 10 mm | -13861 CHF11306 € | |
| SF00S06-1020WT | BioPro IEX SF, non-porous, semi-preparative HPLC Column (20 mm ID), S-6 µm, 100 x 20 mm | -27760 CHF22643 € | |
| SF00S06-1030WT | BioPro IEX SF, non-porous, semi-preparative HPLC Column (30 mm ID), S-6 µm, 100 x 30 mm | -55678 CHF45414 € | |
| SF00S06-2546WP | BioPro IEX SF, non-porous, PEEK HPLC column (4.6 mm ID), S-6 µm, 250 x 4.6 mm | -3178 CHF2592 € | |
| SPA0S05-0346WP | BioPro IEX SP, PEEK HPLC column (4.6 mm ID), 100 nm, S-5 µm, 30 x 4.6 mm | -1919 CHF1565 € | |
| SPA0S05-0546WP | BioPro IEX SP, PEEK HPLC column (4.6 mm ID), 100 nm, S-5 µm, 50 x 4.6 mm | -1919 CHF1565 € | |
| SPA0S05-1008WT | BioPro IEX SP, Classical Analytical HPLC Column (8.0 mm ID), S-5 µm, 250 x 8.0 mm | -6714 CHF5476 € | |
| SPA0S05-1046WP | BioPro IEX SP, PEEK HPLC column (4.6 mm ID), 100 nm, S-5 µm, 100 x 4.6 mm | -2068 CHF1687 € | |
| SPA0S06-1010WT | BioPro IEX SP, semi-preparative HPLC Column (10 mm ID), S-06 µm, 100 x 10 mm | -11670 CHF9519 € | |
| SPA0S06-1020WT | BioPro IEX SP, semi-preparative HPLC Column (20 mm ID), S-06 µm, 100 x 20 mm | -21773 CHF17759 € | |
| SPA0S06-1046WT | BioPro IEX SP, Classical Analytical HPLC Column (4.6 mm ID), S-6 µm, 250 x 4.6 mm | -2050 CHF1672 € | |
| XRPRCP02 | Precolumn Filter 2 µm for 1/16" OD + Holder, PEEK (350 bar) | -330 CHF269 € | |
| XRPRCP25 | Replacement Precolumn Filter 2 µm for 1/16" OD, PEEK (350 bar) | -240 CHF196 € |
