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Use our Application Search to discover chromatography solutions for HPLC and UHPLC. Filter by industry, compound, or column type to find the ideal match for your analysis.
Advantages
YMC BioPro HIC Columns
High resolutions
Short run times
High throughput
Excellent lot-to-lot reproducibility
Long term stability
No carryover effects
High pressure tolerance
Optimized for antibodies and ADCs
PRODUCT MEDIA
Gentle Separation for
Protein Purification
Downloads
YMC Hydrophobic Interaction Columns
Download our detailed product brochure for in-depth insights into YMC Biochromatography columns or access the complete media package with extended materials.
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Applications
| Application | Column | Compound name | |
| Antibody drugs by HIC using Isopropanol | BioPro HIC BF | Adalimumab (Humira®) Brentuximab Pembrolizumab (Keytruda®) Reslizumab (Cinqaero®) | |
| Antibody drugs by HIC using ammonium sulphate | BioPro HIC BF | Bevacizumab (Avastin®) Rituximab (MabThera®) NIST mAb Trastuzumab (Herceptin®) Adalimumab (Humira®) | |
| Antibody drugs by HIC using sodium chloride | BioPro HIC BF | Bevacizumab (Avastin®) Rituximab (MabThera®) NIST mAb Trastuzumab (Herceptin®) Adalimumab (Humira®) | |
| Drug-to-Antibody Ratio (DAR) analysis of ADC | BioPro HIC BF | Cysteine-conjugated ADC mimic | |
| High throughput DAR determination of brentuximab vedotin (Adcetris®) by reducing the analysis time | BioPro HIC HT | Brentuximab vedotin (Adcetris®) | |
| Online native HIC-MS analysis of a monoclonal antibody mixture | BioPro HIC BF | 7 different Monoclonal Antibodies | |
| Online native HIC-MS analysis of a monoclonal antibody molecular variants | BioPro HIC BF | 2 different Monoclonal Antibodies | |
| Online native HIC-MS analysis of cys-linked ADCs | BioPro HIC BF | Cysteine conjugated ADC mimic deglycosylated | |
| Protein Analysis by HIC | BioPro HIC BF | Myoglobin Ribonuclease A Lysozyme α-Chymotrypsinogen A |
Specifications
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BioPro HIC HT
|
BioPro HIC BF
|
|
| Base Material | hydrophilic polymer (polymethacrylate) | |
| Particle Size [µm] | 2.3 | 4 |
| Pore Size | non-porous | |
| pH Range | 2.0 - 12.0 | |
| Pressure Limit | 40 MPa / 5,800 psi | 20 MPa / 2,900 psi |
| Temperature Range | 10 - 60°C | |
Phase Details
BioPro HIC HT
| Base Material | hydrophilic polymer (polymethacrylate) |
| Particle Size | 2.3 µm |
| Pore Size | non-porous |
| Modification | butyl |
| pH Range | 2.0 - 12.0 |
| Pressure Limit | 40 MPa / 5,800 psi |
| Temperature Range | 10 - 60°C |
BioPro HIC BF
| Base Material | hydrophilic polymer (polymethacrylate) |
| Particle Size | 4 µm |
| Pore Size | non-porous |
| Modification | butyl |
| pH Range | 2.0 - 12.0 |
| Pressure Limit | 20 MPa / 2,900 psi |
| Temperature Range | 10 - 60°C |
Technical Library
| Title | Document Type | Language | Download |
 Analysis of oxidised monoclonal antibodies using BioPro HIC BF |
Application Note | EN | |
| HIC Analysis of Different Monoclonal Antibodies using Isopropanol as Modifier |
Application Note | EN | |
| HPLC Analysis of Drug-to-Antibody Ratio with BioPro HIC BF by YMC |
Application Note | EN | |
| High throughput DAR determination of brentuximab vedotin using YMC's BioPro HIC HT |
Application Note | EN | |
| Online native HIC-MS analysis of MAbs and their molecular variants using BioPro HIC BF |
Application Note | EN | |
| Online native HIC-MS analysis of cys-linked ADCs using BioPro HIC BF |
Application Note | EN | |
| Separation of plasmid isoforms using BioPro HIC BF |
Application Note | EN | |
| The right HIC column for each separation- BioPro HIC HT and BioPro HIC BF |
Application Note | EN | |
| YMC Application Collection - Antibodies & ADCs |
Application Note | EN | |
 BioPro HIC HT and BioPro HIC BF Care and Use |
Care and Use Instructions | EN | |
 YMC Biochromatography Columns |
Catalogue | EN | |
| YMC Biochromatography Columns - HIC Columns Chapter |
Catalogue | EN | |
 Efficient HIC plasmid isoform analysis |
Expert Tip | EN | |
 Analytical Stationary Phases for BioLC from YMC |
Overview | EN | |
| YMC Alternatives to Existing BioLC Columns |
Overview | EN | |
 The Principles of BioLC & YMC BioLC Phase Overview |
Poster | EN | |
 About the resolving power of non-porous HIC columns for intact protein analysis |
Technical Note | EN | |
| Characterisation of mAbs and related products via HIC-MS |
Technical Note | EN | |
| Charakterisierung von monoklonalen Antikörpern und verwandten Produkten via HIC-MS |
Technical Note | DE | |
| Strategies to Improve Your HIC Analysis |
Technical Note | EN | |
| Welcher LC-Modus ist besser für verschiedene ADC-Typen geeignet? RP oder HIC? |
Technical Note | DE | |
| Which LC-mode is more suitable for different ADC-types? RP or HIC? |
Technical Note | EN | |
 Grundlagen der Chromatographie für die Analyse und Aufreinigung von Biologika |
Whitepaper | DE |
Ordering Information
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- 2-3 days
- 1-2 weeks
- 3-4 weeks
| Product | Description | Price | Stock |
| BHB00S04-1010WT | BioPro HIC BF, non-porous, semi-preparative HPLC column (10 mm ID), S-4 µm, 100 x 10 mm | - 15291 CHF 12472 € | |
| BHB00S04-1046WT | BioPro HIC BF, non-porous, HPLC column (4.6 mm ID), S-4 µm, 100 x 4.6 mm | - 2203 CHF 1797 € | |
| BHH00SQ3-1046PTH | BioPro HIC HT, non-porous, HPLC column (4.6 mm ID), S-2.3 µm, 100 x 4.6 mm | - 2441 CHF 1991 € | |
| BHH00SQ3-H346PTH | BioPro HIC HT, non-porous, HPLC column (4.6 mm ID), S-2.3 µm, 33 x 4.6 mm | - 1884 CHF 1537 € | |
| XRPRCP02 | Precolumn filter 2 µm for 1/16" OD + holder, PEEK (350 bar) | - 370 CHF 302 € | |
| XRPRCP25 | Replacement precolumn filter 2 µm for 1/16" OD, PEEK (350 bar) | - 271 CHF 221 € | |
| XRPRCS03 | Precolumn filter 0.5 µm for 1/16" OD + holder, stainless steel (600 bar) | - 370 CHF 302 € | |
| XRPRCS35 | Replacement precolumn filter 0.5 µm for 1/16" OD, stainless steel (600 bar) | - 271 CHF 221 € |
Guide to HIC Columns for HPLC & UHPLC
What Are HIC Columns?
Hydrophobic Interaction Chromatography (HIC) is a widely used bioseparation technique that enables the purification of biomolecules such as proteins, monoclonal antibodies (mAbs), and antibody-drug conjugates (ADCs). Unlike reversed-phase chromatography, HIC operates under mild conditions, helping to maintain the native structure and biological activity of sensitive biomolecules.
How Do HIC Columns Work?
HIC Columns separate biomolecules based on their hydrophobicity. In high-salt environments, hydrophobic interactions between the analyte and the stationary phase are enhanced, promoting binding. As the salt concentration gradually decreases, the biomolecules elute according to their hydrophobicity, enabling high-resolution separation. YMC’s BioPro HIC HT is designed for high-throughput separations, offering rapid analysis at increased flow rates without compromising resolution. BioPro HIC BF provides optimized surface modification, ensuring excellent peak shapes and reproducibility, even at higher pressures.
Applications of HIC in Bioseparations
- Purification of monoclonal antibodies (mAbs) and ADCs
- Separation of protein variants and aggregates
- High-throughput analysis with increased flow rates
- Preserving native protein conformation in mild separation conditions
For expert advice on choosing the right HIC column for your application, contact YMC Europe today.