YMC-Pack Pro C18 is based on an ultra pure silica support, which is used for the whole ProFamily. Due to a proprietary endcapping process especially designed for this type of silica, YMC-Pack Pro C18 is perfectly suitable for the separation of acidic and basic molecules. The inertness of the silica makes it an excellent choice for the analysis of drugs or metabolites, compounds that are susceptible to polar interactions with residual silanol groups and metal impurities.

YMC-Pack Pro C18 is also available in 2 µm particle size: YMC UltraHT Pro 18. This allows the medium balance of hydrogen-bonding capacity and hydrophobicity of this phase, to be used for ultra-fast LC.

The separation of polar compounds in many cases requires highly aqueous mobile phase conditions to achieve sufficient retention on the stationary phase. Conventional reversed phase selectivities do not give reproducible results under these conditions mainly due to the collapse of the C18 chains. Hydrosphere C18 has been developed, on the ultra pure silica support of the ProFamily, to be stable in 100% aqueous eluents. For ultra-fast LC applications YMC-Ultra HT Hydrosphere C18 with 2 µm particle size provides the optimal selectivity for the separation of highly polar compounds.

The relatively high carbon load of 22% for YMC-Pack Pro C18 RS and its smaller pore diameter amplifies the selectivity’s ability to discriminate between closely related compounds such as positional or steric isomers. YMC-Pack Pro C18 RS recognises even slight steric differences, even if the analytes differ only in their three-dimensional structure and not in their hydrophobicity or polarity. A more conventional carbon load (~15%) C18 chemistry is not able to use this property for separation.

YMC-Pack Pro C8 is one of YMC’s most commonly used bonded phases and an excellent alternative to C18 selectivities. Due to its moderate hydrophobicity, retention times tend to be shorter than those for ODS phases. YMC-Pack Pro C8 is suitable for a wide range of sample types including pharmaceuticals and biologicals with a relatively high hydrophobicity.

With Pro C4, YMC offers a stationary phase based on the well-known ultra pure silica of the ProFamily. Compared to a C18 phase with the same eluent, C4 phases are less hydrophobic and gives shorter retention times for non-polar compounds while the retention time of polar analytes are virtually unaffected. This makes the Pro C4 an interesting alternative especially when short analysis times are required.