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Guide to Chiral Columns for HPLC & SFC

What Are Chiral Columns?

Chiral columns are specialized chromatographic columns designed for the separation of enantiomers—molecules that are mirror images of each other but cannot be superimposed. In chiral chromatography, these columns play a crucial role in pharmaceutical and chemical research, where precise enantiomer separation is essential for safety and efficacy. YMC offers high-performance chiral columns packed with selectors that are either coated, immobilized, or bonded to the support material, each providing different interaction mechanisms with chiral analytes.

Chiral Separation Mechanisms

Chiral separation in HPLC and SFC depends on the interaction between the chiral selector and the enantiomers in the sample. Depending on the selector used, chiral column chromatography can be performed in:

  • Normal Phase (NP) mode

  • Reversed Phase (RP) mode

  • Supercritical Fluid Chromatography (SFC) mode

YMC’s chiral columns are available with various pore sizes and selector chemistries, ensuring broad applicability and excellent selectivity. This flexibility enables optimized separation of enantiomers based on hydrogen bonding, steric hindrance, and π-π interactions.

Applications of Chiral Chromatography

Chiral column chromatography is widely used in:

  • Pharmaceutical quality control and drug development

  • Stereoisomer analysis in active pharmaceutical ingredients (APIs)

  • Enantiomeric purity determination in research and production

  • SFC method development for high-throughput enantioseparation

With their robust performance and reproducibility, YMC chiral columns are ideal for labs requiring precision in enantiomeric separations across a range of chromatographic conditions.

 

Need help selecting the right chiral column for your HPLC or SFC application? Contact YMC Europe for tailored recommendations.

Contact us for consultation
  • YMC Europe GmbH
  • Phone +49 2064 427-0
  • Schöttmannshof 19
  • Fax +49 2064 427-222
  • D-46539 Dinslaken